LOCUS       sequence-373463-       12656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged dCas12j2 VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12656)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 12656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: dCas12j2 VPR
FEATURES             Location/Qualifiers
     source          1..12656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4715..4762
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4763..4786
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4829..4853
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4883..5032
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5057..5077
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5507..5863
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5882..6451
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             6461..6517
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6518..7114
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7011..7031
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7130..7718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7183..7203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7601..7612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7790..8023
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8049..8066)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8055..8279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8325..8753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8412..8431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8622..8643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8762..8782)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8767..9096
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8947..9082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9009..9028
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9144..9191
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9210..9584
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9714..9835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9751..9770)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9805..9824
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9884..9900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9884..9900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9897..9919)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9908..9924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9932..9938,9939..9956,9957..9962))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9977..9998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10115..10132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10286..10874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10366..10385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11045..11836,11837..11905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11668..11687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11906..12010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12085..12104)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12276..12655
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatgccaa agccagccgt
     2461 ggagtctgag ttttctaagg tactcaagaa gcactttccg ggcgagcgat ttaggtctag
     2521 ctacatgaag cggggtggta aaatcttggc agcccagggt gaagaagcgg tcgtcgcgta
     2581 tctgcaaggc aagtccgagg aggaaccccc gaattttcag ccgccggcga aatgtcatgt
     2641 tgttacgaaa tcacgagatt tcgccgagtg gccaattatg aaggcctccg aagcaatcca
     2701 aaggtatatc tatgcgctct ctacgacgga acgggcagct tgcaagcctg gcaaatcttc
     2761 agagtcccac gcggcctggt tcgcggcaac tggcgtgtca aaccacggtt atagccatgt
     2821 tcaaggcctc aatcttatct tcgaccacac gctgggaaga tacgatggtg ttctgaaaaa
     2881 ggtgcagctg agaaatgaga aagcccgcgc ccggctggaa agtatcaacg cctctcgagc
     2941 cgacgaagga cttccagaaa taaaggcaga ggaggaagag gtcgctacaa atgaaaccgg
     3001 acaccttttg cagcctccgg ggatcaaccc aagtttctac gtttaccaga ctatttctcc
     3061 gcaggcttac aggccgcgag atgagattgt actgccgccc gagtatgccg gctacgtccg
     3121 agatccgaac gcccctatcc cccttggcgt ggttcggaat cggtgcgata ttcagaaggg
     3181 atgccctgga tacatccccg aatggcaaag agaggcaggt actgcaattt cccctaagac
     3241 gggtaaagcc gtcaccgttc ccggcctcag tccaaaaaaa aataaacgaa tgcgacgata
     3301 ctggaggtcc gagaaagaga aggcccaaga tgcactgctc gttactgtga gaatcggcac
     3361 tgactgggtc gtaatcgacg ttcgaggttt gctgcggaat gcgcggtggc gcaccattgc
     3421 gcccaaggat atatccttga atgccctctt ggatctcttt acaggcgacc cggtcataga
     3481 tgttcggaga aacattgtga ctttcaccta cactctggac gcttgcggta catatgctcg
     3541 caaatggact ctcaaaggga aacagactaa ggcaaccctc gataagttga ccgcaaccca
     3601 gaccgtggcc ctggtagcaa tagcccttgg acaaaccaat cccataagtg cgggtatcag
     3661 tagggtcacg caagaaaacg gggcacttca atgtgaacct ctggatcggt tcactctccc
     3721 tgatgatctg ctcaaggata tctccgcgta ccgaatcgct tgggatcgca acgaggagga
     3781 actgagggct aggtccgtcg aagcgctccc agaagctcaa caagctgaag tgagggctct
     3841 ggacggcgtt tctaaagaaa ccgccaggac ccagctctgc gcggacttcg gccttgatcc
     3901 caaacggctg ccttgggata aaatgagcag caacaccact ttcatcagtg aagcgttgct
     3961 tagtaattct gtgtctagag atcaggtttt ttttactcct gcgcctaaaa agggagcaaa
     4021 gaaaaaagcc cccgttgaag ttatgcggaa ggataggacc tgggcgaggg cctataaacc
     4081 acggctcagt gtggaagccc aaaagctgaa aaatgaggcc ttgtgggctc tcaagcgcac
     4141 ttctccagaa tacctcaagc tgagtcggag aaaagaggag ctttgtaggc gaagtattaa
     4201 ctacgtcatt gaaaaaacaa gacggaggac acaatgtcag atcgtgatac ctgtcataga
     4261 ggacttgaat gtgcgattct ttcacggttc agggaagcgc ctgcctggct gggataattt
     4321 tttcactgcg aagaaggaga acaggtggtt tatacagggc ctccacaaag cattcagcga
     4381 cttgcgaact catcgctcct tctacgtatt cgaagtccgc ccggagcgga cttcaataac
     4441 gtgcccaaaa tgcgggcact gcgaggttgg gaaccgggat ggggaggctt ttcagtgcct
     4501 tagttgcggc aaaacgtgca atgccgacct tgacgtggct acccataatc tgactcaagt
     4561 cgcccttaca ggaaaaacaa tgccgaaacg cgaggaacct agagatgccc agggcacagc
     4621 tccagcccga aaaacaaaga aggcgtcaaa gagcaaggct ccgccagccg aacgagagga
     4681 ccaaactcca gcacaggaac cgtcccagac ttccaagcga cctgccgcca caaagaaggc
     4741 tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat cctcgccagg
     4801 gatccgtcga cttgacgcgt tgatatcaac aagtttgtac aaaaaagcag gctacaaaga
     4861 ggccagcggt tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag
     4921 tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga
     4981 cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaactc
     5041 tagaagttcc ggatctccga aaaagaaacg caaagttggt agccagtacc tgcccgacac
     5101 cgacgaccgg caccggatcg aggaaaagcg gaagcggacc tacgagacat tcaagagcat
     5161 catgaagaag tcccccttca gcggccccac cgaccctaga cctccaccta gaagaatcgc
     5221 cgtgcccagc agatccagcg ccagcgtgcc aaaacctgcc ccccagcctt accccttcac
     5281 cagcagcctg agcaccatca actacgacga gttccctacc atggtgttcc ccagcggcca
     5341 gatctctcag gcctctgctc tggctccagc ccctcctcag gtgctgcctc aggctcctgc
     5401 tcctgcacca gctccagcca tggtgtctgc actggctcag gcaccagcac ccgtgcctgt
     5461 gctggctcct ggacctccac aggctgtggc tccaccagcc cctaaaccta cacaggccgg
     5521 cgagggcaca ctgtctgaag ctctgctgca gctgcagttc gacgacgagg atctgggagc
     5581 cctgctggga aacagcaccg atcctgccgt gttcaccgac ctggccagcg tggacaacag
     5641 cgagttccag cagctgctga accagggcat ccctgtggcc cctcacacca ccgagcccat
     5701 gctgatggaa taccccgagg ccatcacccg gctcgtgaca ggcgctcaga ggcctcctga
     5761 tccagctcct gcccctctgg gagcaccagg cctgcctaat ggactgctgt ctggcgacga
     5821 ggacttcagc tctatcgccg atatggattt ctcagccttg ctgggctctg gcagcggcag
     5881 ccgggattcc agggaaggga tgtttttgcc gaagcctgag gccggctccg ctattagtga
     5941 cgtgtttgag ggccgcgagg tgtgccagcc aaaacgaatc cggccatttc atcctccagg
     6001 aagtccatgg gccaaccgcc cactccccgc cagcctcgca ccaacaccaa ccggtccagt
     6061 acatgagcca gtcgggtcac tgaccccggc accagtccct cagccactgg atccagcgcc
     6121 cgcagtgact cccgaggcca gtcacctgtt ggaggatccc gatgaagaga cgagccaggc
     6181 tgtcaaagcc cttcgggaga tggccgatac tgtgattccc cagaaggaag aggctgcaat
     6241 ctgtggccaa atggaccttt cccatccgcc cccaaggggc catctggatg agctgacaac
     6301 cacacttgag tccatgaccg aggatctgaa cctggactca cccctgaccc cggaattgaa
     6361 cgagattctg gataccttcc tgaacgacga gtgcctcttg catgccatgc atatcagcac
     6421 aggactgtcc atcttcgaca catctctgtt tggatccggc gcaacaaact tctctctgct
     6481 gaaacaagcc ggagatgtcg aagagaatcc tggaccgacc gagtacaagc ccacggtgcg
     6541 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6601 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6661 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6721 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6781 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6841 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6901 agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6961 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7021 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7081 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt taagtcgaca atcaacctct
     7141 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7201 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7261 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7321 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7381 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7441 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7501 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7561 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7621 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7681 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     7741 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7801 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     8041 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8101 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8161 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8221 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     8281 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     8341 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8401 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8461 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8521 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8581 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8641 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8701 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     8761 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8821 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8941 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9001 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     9061 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     9121 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     9181 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     9241 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     9301 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     9361 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     9421 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     9481 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     9541 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     9601 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     9661 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     9721 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9781 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9841 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9901 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9961 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    10021 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    10081 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10141 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10201 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10261 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10381 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10441 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10501 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10561 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10621 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10681 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10741 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10801 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10861 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10921 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10981 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    11041 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    11101 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    11161 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    11221 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11281 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11341 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11401 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11461 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11521 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11581 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11641 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11701 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11761 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11821 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11881 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11941 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    12001 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    12061 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    12121 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    12181 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
    12241 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
    12301 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    12361 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    12421 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    12481 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    12541 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    12601 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgg
//
