LOCUS       sequence-37347-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas 
            E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM
  TITLE     mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP 
            Pathway.
  JOURNAL   Cell. 2011 Aug 5;146(3):408-20.
  PUBMED    21816276
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-08-5"; volume: "146(3)"; pages: "408-20"
COMMENT     Sequence Label: pLKO-puro FLAG SREBP2
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     270..289
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             348..371
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct gttngtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatanaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc
      121 ggattccccg tgccaagagt gacgtaagta ccgcctatag agtctatagg cccaccccct
      181 tggcttcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
      241 ttaggtgaca ctatagaata acatccactt tgcctttctc tccacaggtg tccactccca
      301 ggtccaactg cacctcggtt ctatcgattg aattcaccat ggaaatggac tacaaggatg
      361 acgatgacaa gggtggtggt ggtggtgcgt cgacgatgga tgagagcagc gagctgggcg
      421 ttctggagac catggagacc ctcacggagc tgggcgatga gctgactctc ggggacatcg
      481 acgagatgct acagtttgtc agcaatcaag tgggagagtt ccctgatttg ttttcagagc
      541 aactttgtag ctccttccct ggaggtggca gcaatggtgg cagtggcaac aacagcagtg
      601 gcagaggcaa caatggtgga gccacagacc ctgcagtaca gcggtcattc agccaggtcc
      661 cattatctac cttctctccc tcagctgcct cccctcaggc tccggccctg caggtcaaag
      721 tctctcctac cccaccaagg gcaacccctg ttctccagcc gcgaccccag ccccagcccc
      781 aacctccagc tcagctgcag cagcagacgg tgatgatcac cccgacgttc agcaccgctc
      841 cgcagacgag gatcatccag cagcctttga tataccagaa cgcagcgacc agctttcaag
      901 tcctgcagcc tcaagtgcaa
//
