LOCUS       sequence-373470-       12427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Kingston ER, Blodgett LW, Bartel DP
  TITLE     Endogenous transcripts direct microRNA degradation in Drosophila, 
            and this targeted degradation is required for proper embryonic 
            development.
  JOURNAL   Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029.
  PUBMED    36150386
REFERENCE   2  (bases 1 to 12427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029."
COMMENT     Sequence Label: doraWT_rescue_construct
FEATURES             Location/Qualifiers
     source          1..12427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7845..7898
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7923..8522
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7923..7942)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8419..8439
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8530..8664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8554..8573)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8608..8627
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9265..9853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9345..9364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10024..10815,10816..10884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10647..10666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10885..10989)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11057..11075
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_RNA        complement(11227..11302)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atgctcaacg caccgcatgt tgccgtttcc ttttatgcgt cattttggct cgaaataggc
       61 aattatttaa acaaagatta gtcaacgaaa acgctaaaat aaataagtct acaatatggt
      121 tacttattgc catgtgtgtg cagccaacga tagcaacaaa agcaacaaca caggtggctt
      181 tccctctttc actttttgtt tgcaagccgc gtgcgagcaa gacggcacga ccggcaaacg
      241 caattacgct gacaaagagc agacgaagtt ttggcgaaaa acatcaaggc gcctgatacg
      301 aatgcatttg caataacaat tgcgatattt aatattgttt atgaagctgt ttgacttcaa
      361 aacacacaaa aaaaaaaata aaacaaatta tttgaaagag aattaggaat cggacgctta
      421 tcgttagggt aacaacaaga aatgcttact gagtcacagc ctctggaaaa ctgccgcaag
      481 ccagagagag agagaaaaag agggagagca gcttagaccg catgtgcttg tgtgtgaggc
      541 gtctctctct tcgtctctgt tgcgcaaacg catagactgc actgaaaaaa tcgattacct
      601 attttttatg aatgaatatt tgcactatta ctattcaaaa ctattaagat agcaatcaca
      661 ttcaatagcc aaatactata ccacctgagc gatgcaacga aatgatcaat ttgagcaaaa
      721 atgctgcata tttaggacgg catcattata gaaatgcttc ttgctgtgta cttttctctc
      781 gtctggcagc tgtttcgccg ttattgttaa aaccggctta agttaggtgt gttttctacg
      841 actagtgaat gccctactag aagatgtgtg ttgcacaaaa tgtccctgga ataaccaatt
      901 tgaagtgcag atagcagtaa acgtaagcta atatgaatat tatttaactg taatgtttta
      961 atatcgctgg acattactaa taaacccact ataaacacat gtacatatgt atgttttggc
     1021 atacaatgag tagttgggga aaaaatgtgt aaaagcaccg tgaccatcac agcataaaga
     1081 taaccagctg aagtatcgaa tatgagtaac ccccaaattg aatcacatgc cgcaactgat
     1141 aggacccatg gaagtacact cttcatggcg atatacaaga cacacacaag cacgaacacc
     1201 cagttgcgga ggaaattctc cgtaaatgaa aacccaatcg gcgaacaatt catacccata
     1261 tatggtaaaa gttttgaacg cgacttgaga gcggagagca ttgcggctga taaggtttta
     1321 gcgctaagcg ggctttataa aacgggctgc gggaccagtt ttcatatcac taccgtttga
     1381 gttcttgtgc tgtgtggata ctcctcccga cacaaagccg ctccatcagc cagcagtcgt
     1441 ctaatccaga gaccccggat cggggtacct actagtccag tgtggtggaa ttcatggacc
     1501 gcttcagctt cgacgactcc atccgcttcg aggaagactc cctctgcagc tggagctctg
     1561 aaccggaatc gctgtgcaac aactggcgcg gctggaagaa gcccgctgca ggcagcggcg
     1621 caggcaatgg cggcttgagc ggcatgggcg gtatggctgt ggccggcggc tcaggagcac
     1681 cgccgttcgg aggagcagga gcctctggtt cctgcacacc gggcggcaat ggaacggcca
     1741 tggccaacac atccacctgc ggcagacatt atgaagtatc cacattggcc gaactggcag
     1801 cccgctgtgt ggcctcctac ataccgttcg agctggttga gcacgtgtat ccgccggtgc
     1861 cggagcagct gcagctgcgc attgcgttct ggagctttcc ggacaacgag gaggacatcc
     1921 ggctctactc ctgtctggcc aatagctcgg cggacgagtt caatcgcggc gactcgttgt
     1981 ttcggttgcg agctgtcaag gatccgctgc agataggctt tcacctctcg gccagcgttg
     2041 tcaatcagac gccgcgtgcc tatttcaatg tggccgtcac ctttgaccgc cgacgcatct
     2101 cctcttgcaa ctgcacctgc acctcgtccg cctactggtg ctcccacgta gtcgccgttt
     2161 gtctgcatcg catccattgc ccccaggagg tctgtctgcg tgccccggta tcggaatcat
     2221 tgactcgcct gcaacgcgac caattgcaaa agtttgccca gtatctgatc agcgagctgc
     2281 ctcagcagat cttgccgacg gctcagcggc tcctggatga gttgctcagt gcgcaaccca
     2341 cggcgatcaa cactgtgtgc ggtgcacctg atcccacggc tggggcctcg atcaacgacc
     2401 agaccagctg gtatctggac gagaagacgt tgcataacaa cattaaacga atcctcatca
     2461 agttctgttt gcccgccccg attgtgttta gcgacgtaaa ctatctgacg aactcggctc
     2521 ccccagcggc ggccgaatgg agctcgctac tccgaccgct gcggggacgt gagccggagg
     2581 gcatgtggaa tctactttcg atcgtacgcg agatgtacag gcgctgtgat cggaacgcag
     2641 tgcgtctgct ggagatcatc acggaggagt gcttgtactg cgatcagata ctcatctggt
     2701 ggttccagac gaagttggca ctgatgatgg ggtcccacgg ccactccggc ggtaagcatt
     2761 ctaacactca ctccaactcc acggcactgc agcatgcttg cagttcgctg tgcgacgaaa
     2821 tcgtggcact gtggcgattg gcagcgctca atcccggcct ggcgccggat gagcgagata
     2881 tgctgcacgc tcagttcacg gcctggcatc taaagattct tgaccgggtg gtcaagagcc
     2941 gaatgatgcc gtcgtacacc aacaagcacc agcagaactc gcgatccgag acggagctgt
     3001 ttatcggctt caagccggcc atcgaggcgt gctacttgga ctgggagggc taccccatac
     3061 cgggagtgac gcacacccac gacacgaatc ccatctacta ctcgccgttc acttgcttca
     3121 agcacacgga cctcaagggc gagtctaaca atcccggcca gctgaatgcc acccaagcgc
     3181 tgatgtcgaa caacaagcac tacaattact ttagttcatc cagcgatcat ggcatgcacg
     3241 caggagcctt caagcagagg ttggaccggc cttttcgtga gtcgcgattc tatacgaatc
     3301 cggccgatct ggacggagga gcagcgttgg cgggcggagg atccggtagg ttttccacgg
     3361 gactgggctc cgttgccgtt ggaggagctg gtgcaggagg cttgggtcag atgagcggag
     3421 gagttaacaa ctctgttgga ggatcagcgg ccgccgaagt gaatcccgtg cagcagcagc
     3481 ttcccagtgg ctcggcggga gtcggtgcct ctgtgacaaa tgccaaggtt gtggtagcaa
     3541 cagatggcaa tcgttcgagt gccagcagcg agggattctg cgagaacgac gatttcggcg
     3601 gcgacaccag cagcagtcac aactactgtc cacctggaca ggcggttgtg cctggccaga
     3661 aatcagccct cacggagtcg gattcgcaga gcagcttcga tgcagtgtcg catcagagca
     3721 aggatgagcc accagtcggg ggagtgggtg tggcagtggg tgttgaacaa gccctttcca
     3781 catctgacac ttcgtcggcc tcctcctcat cgtcatctgc ttctacagct tcagggagct
     3841 ctaatagttc cacttcctct atgcttatgg ctggacagga ggctactgtc ggtgttggtg
     3901 gcgctgtgca gcaaacaact cgtcgcctca gcaaggatga atcctttagt agcagtagcg
     3961 acgagttcaa ccagggaggc gtgggtggat cagcaggtcg agtggctatg gcctccagtg
     4021 gaagcggatc gggtgctggc gacacagtag cggtgggatc gggatcaaca gctggcggag
     4081 atctgacgcc ggtgcccagc acgtcggcag ctgcccgtgc tgccttggcc gccagctcga
     4141 cagggccggt gatttctgct tcacctcatc tcgcagctag cgccgctgga ggcttaggag
     4201 tgctgggtgt gggtgcagac cagccttgca cctcatccgc gcatgcagcc cgagcactgg
     4261 cagctgcagt ggctgcttcc agcgataagc cgcatgtttt ctccaatgta cggcccacag
     4321 aggacgcctg ggatatactg ctggccagag ccgagggttt gcatgcccac ggtcacggag
     4381 ccgaggcttg catcctggcc gtccgtctgg ccgaacagat gctggcgaat ccgcctaacc
     4441 tacttctgga actgccaccc gcacccaaaa gaaagggcaa gaagcaaaac gtgaatccca
     4501 tctcgcatca gctgacagtt gtggcctctg caacgctgtc caagtgcgct tttctgtgca
     4561 cagtgctgtc ggagaactct gaacactatc acattggctt tcggatatgc ctattcgcct
     4621 tggagatgcc gcgaccgcca gcaagcacca agcccctgga agtcaagctg gccaaccagg
     4681 aggcggacat actggctcta ctaaagcgcc tgccgctggg atccgcggaa ctgcaagtaa
     4741 tccgagagcg agctgagcaa ctgcgcagcg gaacctttaa gacgcgagga gaggctctat
     4801 tgcctattaa cctggccacg tttattttcg atgccctagt tacactcagc cccttaggtg
     4861 gatccacaat tgtccctggc gtggcatcca ctagtggtgt atcaagtgcg gccggcaagt
     4921 ctatggtcaa tacaggcaca cgtttgctgc tctacaagca caacagcgat gagagtctgg
     4981 gattcgatgc tgcagtggct gctctcggac taaaagccaa tgtgtcggag gcagagcatc
     5041 cgttgttgtg cgaaggaacc cgccggcagc gcggggatct tgctctgaca ttgctttcac
     5101 actacaagga tgagccccgc aagatagcca agatcatgga gaagcttctg gacagggaca
     5161 tacatacgct tctaaatgcg ccactcctgc ccgcttacta ctcgagtaac ccgcctgtga
     5221 ggacgcgaag taatcagccc acgcgtaggg aggatcacga ttatggcggt ggctctggat
     5281 gcgcctcttc caactgcaat ccggtagcca atttgtgtga acttttgccg gcggactacg
     5341 gcagtgtggg tggaaacagt cgaccacaca gctccacgag tgccgaactg gaactaagca
     5401 tgtgtgccct gagcatggcc agcagtggta gccagtcggg aggcgttcaa ggcatggtgc
     5461 ccacaacgaa tgcagcggga accacgggaa cacccagttc cagcagcacg acggtcagtg
     5521 ggagccagaa tcccaatggt aatcccagtg gcagcggtgg aggaggaaat ggcggaggtg
     5581 gtaacggagg aggaggaggc ggtggcggtg gtggtggagg ctccaccagt agccgaagca
     5641 aggaaagccg gtacaaggga aagagagcat atccctcgat acccaaccag ccatcggaag
     5701 ccagtgctca ctttatgttc gagctggcca agaatgtgct gaccaaggcc ggcggcaata
     5761 gttccacatc gctcttcacc caggcgagca cgagccaaaa tcaccatgga ccgcaccgtg
     5821 ccctccacat gtgcgccttc caactagggc tgtatgccct cggtctgcac aactgtgtga
     5881 gtcccaattg gctatcgagg acgtactcgt cgcacgtctc ctggatcctg gggcaagcta
     5941 tggaaattgg tgcgccggcg ataagctttc tgatagacac ttgggaggcg catctaacgc
     6001 ctcccgaagc agctggcatg gccgatcgtg cgtcgcgcgg atgggacagc aacatggtct
     6061 atccggcggc ggagctggct ctgtccgttc tgccccacgc agctgccctc aatcccaacg
     6121 agatccagcg tgcgattctg cagtgcaagg agcaaagcga tctgatgctg gagcgggcct
     6181 gtctcaccgt ggagacagct gccaagggcg gcggcgtcta tccggaggtg ctattccagg
     6241 tggcccgcta ctggtacgag ctgtacatgc ggaacacgcc gaataacagc gagttcgagc
     6301 cgcacgatga caccatggac cattcggccg tgagtctgag cgccctcatt gagtcccagc
     6361 agcagcatga gctccagcaa caacaacagg cagtgcagca gcaacaggcg gttcagcagc
     6421 agcagcaggt ggtgcagcaa cagcagcaag tggtgcagca gcaacaacag cttggcatgc
     6481 ccaattccgt ggtgccaggc ggcgttggcc ccggccccgg actacccgta gcagtgcagc
     6541 cacccgtagt tggttctgtc cagaatccac aggcccaatt ccaacccgtg ggcgtcgcct
     6601 cactggctcc cctcggactg gcgcagtatc caccgtacag cttctgccag ggcttgtatg
     6661 cgcaccacca caacatgagc tatccgcccg ggcagatgca gatgttcatt tcggcagggc
     6721 cgccgccgcc acctcaagca tacggcggct accagcagcc gccgccacag cagccgccga
     6781 atccgcagca acagcagcag gttgtgcaac agcaggtgca gcagcagcag gtggtgcaac
     6841 agcaacagca gcaggttcag atggccgggc aggcgccacc aggtcaccct gggcagcaag
     6901 gacatccagg ccaaccgccc tctggcttcc agccgcaacc accgccagga gcattccagg
     6961 cgctgccgcc gcaggcctat caggcaatgc aagcgggtcc cccgggcccg ccgatgggtc
     7021 cgcctcaggg ctactatggt ccaccaccac caccgcctcc gaacggtccg cctggcgtgg
     7081 gcgtgggcgt cggagtcggc gttggagtgg gcgtgatgcc aatgcgccag catcagcacc
     7141 aacatcccgt ttacccattc atgcagcagg cgccgccgca gccaccgcag cagcaacaac
     7201 cgccgccctc gcagccaccc gtgcgacaac gacaaccaca tcagtttaca cccacccagc
     7261 tgcgatatct gctggctgcc tacaatgtgg gcatgctggc catggaaaca ctggcgcgac
     7321 gcgtccacga cgatcggccg caggcgaagt atgcccgcaa tccgccgtac ggcgaggatg
     7381 tgaaatggtt gctacggatc agcaagaaac tgggcaccca gtatctgcac cagttctgca
     7441 tctgtgcggt caactcgatc gtgagtccgt tcgtgctgca tgacgtggcc attgagtccg
     7501 cccactatct gggcaggaac aaccaccaga tggtgatgca gcacctgcgt tctgcgctca
     7561 cgcctctcgt ccagaagtgt cagcagatgt acatccaatg catccatcag aagctgtatc
     7621 atctgaccca gggcgactat gaggagtttg ccagcattgt agtggcggca cgcgccgctt
     7681 tccagatcac gccggagggg aacgcgcaat tcaaggactg gctgcagtct atcaagcgat
     7741 cgaaatcatg caaaaaggag ctgtggaccc agatcaatgc ggccctgcag agcaactcca
     7801 aaaaggccgg ccaggcaaaa aagaaaaagg gatcaggcaa gcttgagggc agaggaagtc
     7861 ttctaacatg cggtgacgtg gaggagaatc ccggccctgc tagcggtagc ggcagcggta
     7921 gcatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     7981 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     8041 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8101 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     8161 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8221 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8281 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8341 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8401 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8461 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8521 gactcgaggg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     8581 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     8641 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     8701 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     8761 gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     8821 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     8881 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     8941 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     9001 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     9061 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     9121 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     9181 caggcaggta gatgacgacc atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg
     9241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9961 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10021 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10081 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10141 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10201 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10261 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10321 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10381 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10441 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10501 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10561 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10621 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
    10681 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10741 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10801 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10861 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10921 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10981 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    11041 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattaattct
    11101 atattctaaa aacacaaatg atacttctaa aaaaaaatca tgaatggcat caactctgaa
    11161 tcaaatcttt gcagatgcac ctacttctca tttccactgt cacatcattt ttccagatct
    11221 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
    11281 ttgctatttc tagctctaaa actacataac gaataaaaaa ggatttaagt gcaaatggta
    11341 aaaaagtgtc gagtttttct tgagttgatt gtgctgtaat gagactctgc attcggcatt
    11401 tgactcggct tttcctactc gtgccgtatt tcaggctgca agtcgaacag atctcgctgc
    11461 ctgttatgtg gcccacaaac caagacacgt tttatggcca ttaaagctgg ctgatcgtcg
    11521 ccaaacacca aatacataat gaatatgtac acattcgaga aagaagcgat caaagaagcg
    11581 tcttcgggcg gagtaggaga atgcggagga gaaggagaac gagctgatct agtatctctc
    11641 cacaatccaa tgccaactga ccaactggcc atattcggag caatttgaag ccaatttcca
    11701 tcgcctggcg atcgctccat tcttggctat atgtttttca ccgttacccg gggccatttt
    11761 caaagactcg tcggcaagat aagattgtgt cactcgctgt ctctcttcat ttgtcgaaga
    11821 atgctgagga atttcgcgat gacgtcggcg agtattttga agaatgagaa taatttgtat
    11881 ttatacgaaa atcagttagt ggaattttct acaaaaacat gttatctata gataattttg
    11941 ttgcaaaata tgttgactat gacaaagatt gtatgtatat ctttaatgta ttctcatttt
    12001 cttatgtatt tataatggca atgatgatac tgatgatatt ttaagatgat gccagaccaa
    12061 aaggcttgaa tttctgcgtc ttttgccgaa cgcagtgcat gtgcaattgt tgttttttgg
    12121 aatattcaat tttcggactg tccgctttga tttcagtttc ttggcttatt caaaaagcaa
    12181 agtaaagcca aaaaagcgag atggcaatac caaatgcggc aaaacggtag tggaaggaaa
    12241 ggggtgcggg gcagcggaag gaagggtggg gcggggcgtg gcggggtctg tggctgggcg
    12301 cgacgtcacc gacgttggag ccactccttt gaccatgtgt gcgtgtgtgt attattcgtg
    12361 tctcgccact cgccggttgt ttttttcttt ttatgctgcg ctctctctag cgccatctcg
    12421 cttacgc
//
