LOCUS       sequence-373471-        4631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs
            of NA segment of Influenza B/Brisbane/60/2008 virus by RNA 
            polymerase I in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Kedia N, Banerjee S, Mondal A
  TITLE     A Comprehensive Roadmap Towards the Generation of an Influenza B 
            Reporter Assay Using a Single DNA Polymerase-Based Cloning of the 
            Reporter RNA Construct.
  JOURNAL   Front Microbiol. 2022 May 25;13:868367. doi: 
            10.3389/fmicb.2022.868367. eCollection 2022.
  PUBMED    35694292
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2022-05-25"; volume: "13:868367. doi"; pages: " 
            10.3389/fmicb.2022.868367. eCollection 2022"
COMMENT     Sequence Label: pHH21-BNA-Luc
FEATURES             Location/Qualifiers
     source          1..4631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(517..2169)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2085..2103
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(2431..2448)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2601..2687
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2779..2806
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2825..2916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2917..2985,2986..3777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3135..3154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3948..4536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4437..4456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcacgatc ctttctggcg agtccccgtg cggagtcgga gagcgctccc tgagcgcgcg
       61 tgcggcccga gaggtcgcgc ctggccggcc ttcggtccct cgtgtgtccc ggtcgtagga
      121 ggggccggcc gaaaatgctt ccggctcccg ctctggagac acgggccggc cccctgcgtg
      181 tggcacgggc ggccgggagg gcgtccccgg cccggcgctg ctcccgcgtg tgtcctgggg
      241 ttgaccagag ggccccgggc gctccgtgtg tggctgcgat ggtggcgttt ttggggacag
      301 gtgtccgtgt cgcgcgtcgc ctgggccggc ggcgtggtcg gtgacgcgac ctcccggccc
      361 cgggggaggt atatctttcg ctccgagtcg gcattttggg ccgccgggtt attagtagta
      421 acaagagcat ttttcagaaa caattaagtt tagtaaggac aattgttcaa acaaaacagg
      481 aacaaagggt ttagaacaga ctcaaccatt cctccattac acggcgatct ttccgccctt
      541 cttggccttt atgaggatct ctctgatttt tcttgcgtcg agttttccgg taagaccttt
      601 cggtacttcg tccacaaaca caactcctcc gcgcaacttt ttcgcggttg ttacttgact
      661 ggcgacgtaa tccacgatct ctttttccgt catcgtcttt ccgtgctcca aaacaacaac
      721 ggcggcggga agttcaccgg cgtcatcgtc gggaagacct gcgacacctg cgtcgaagat
      781 gttggggtgt tggagcaaga tggattccaa ttcagcggga gccacctgat agcctttgta
      841 cttaatcaga gacttcaggc ggtcaacgat gaagaagtgt tcgtcttcgt cccagtaagc
      901 tatgtctcca gaatgtagcc atccatcctt gtcaatcaag gcgttggtcg cttccggatt
      961 gtttacataa ccggacataa tcataggacc tctcacacac agttcgcctc tttgattaac
     1021 gcccagcgtt ttcccggtat ccagatccac aaccttcgct tcaaaaaatg gaacaacttt
     1081 accgaccgcg cccggtttat catccccctc gggtgtaatc agaatagctg atgtagtctc
     1141 agtgagccca tatccttgcc tgatacctgg cagatggaac ctcttggcaa ccgcttcccc
     1201 gacttcctta gagaggggag cgccaccaga agcaatttcg tgtaaattag ataaatcgta
     1261 tttgtcaatc agagtgcttt tggcgaagaa ggagaatagg gttggcacca gcagcgcact
     1321 ttgaatcttg taatcctgaa ggctcctcag aaacagctct tcttcaaatc tatacattaa
     1381 gacgactcga aatccacata tcaaatatcc gagtgtagta aacattccaa aaccgtgatg
     1441 gaatggaaca acacttaaaa tcgcagtatc cggaatgatt tgattgccaa aaataggatc
     1501 tctggcatgc gagaatctca cgcaggcagt tctatgaggc agagcgacac ctttaggcag
     1561 accagtagat ccagaggagt tcatgatcag tgcaattgtc ttgtccctat cgaaggactc
     1621 tggcacaaaa tcgtattcat taaaaccggg aggtagatga gatgtgacga acgtgtacat
     1681 cgactgaaat ccctggtaat ccgttttaga atccatgata ataatttttt ggatgattgg
     1741 gagctttttt tgcacgttca aaattttttg caaccccttt ttggaaacga acaccacggt
     1801 aggctgcgaa atgcccatac tgttgagcaa ttcacgttca ttataaatgt cgttcgcggg
     1861 cgcaactgca actccgataa ataacgcgcc caacaccggc ataaagaatt gaagagagtt
     1921 ttcactgcat acgacgattc tgtgatttgt attcagccca tatcgtttca tagcttctgc
     1981 caaccgaacg gacatttcga agtactcagc gtaagtgatg tccacctcga tatgtgcatc
     2041 tgtaaaagca attgttccag gaaccagggc gtatctcttc atagccttat gcagttgctc
     2101 tccagcggtt ccatcttcca gcggatagaa tggcgccggg cctttcttta tgtttttggc
     2161 gtcttccatt gttcattttt ggcctatttg cttcagtttt gagaagatgc tctgcttctg
     2221 ctccccccca acttcggagg tcgaccagta ctccgggcga cactttgttt ttttttttcc
     2281 cccgatgctg gaggtcgacc agatgtccga aagtgtcccc cccccccccc cccggcgcgg
     2341 agcggcgggg ccactctgga ctcttttttt tttttttttg gggatcggcc gctagcttgg
     2401 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     2461 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     2521 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     2581 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2641 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     2701 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     2761 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     2821 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2881 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2941 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3001 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3061 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3121 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     3181 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3241 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3301 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3361 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3421 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3481 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3541 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3601 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3661 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3721 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3781 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3901 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4561 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4621 tcattaatgc a
//
