LOCUS       sequence-373476-       11336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged dCas12j3 KRAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: dCas12j3 KRAB
FEATURES             Location/Qualifiers
     source          1..11336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2438..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4742..4789
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4790..4813
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4847..5041
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             5108..5128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5141..5197
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5198..5794
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5691..5711
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5810..6398
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5863..5883)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6281..6292)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6470..6703
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6729..6746)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6735..6959
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7005..7433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7092..7111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7302..7323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7442..7462)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7447..7776
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7627..7762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7689..7708
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7824..7871
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7890..8264
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8394..8515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8431..8450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8485..8504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8564..8580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8564..8580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8577..8599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8588..8604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8612..8618,8619..8636,8637..8642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8657..8678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8795..8812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8966..9554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9046..9065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9725..10516,10517..10585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10348..10367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10586..10690)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10765..10784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10956..11335
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaaa aagaaataac
     2461 tgagctcacc aagattaggc gcgagtttcc gaataaaaag ttcagcagca ctgatatgaa
     2521 gaaggcaggt aagttgttga aggcagaagg tcctgatgct gttagagact tcctgaactc
     2581 ctgccaggag attatcgggg attttaagcc gcctgtaaag acaaacatag tcagcatatc
     2641 acgacccttt gaggagtggc ctgttagtat ggtggggcgc gccatccagg aatattactt
     2701 tagtttgaca aaagaggaat tggagtccgt ccatcccgga acttccagcg aggatcacaa
     2761 gtccttcttt aacataactg gcctgagcaa ttacaattat acgtcagtcc aaggcttgaa
     2821 tctcatcttc aaaaatgcga aggccatata cgacgggact ctggttaaag caaacaataa
     2881 aaataagaag ttggaaaaaa agttcaatga gattaaccac aagcgaagcc ttgaggggct
     2941 tcctataatt acgccggatt tcgaggaacc ctttgatgag aatggccatc tgaataatcc
     3001 gccaggtatt aatcgaaata tttacggcta ccaaggatgt gccgctaaag tattcgttcc
     3061 ttccaagcat aaaatggtat ccctccctaa agaatacgaa gggtacaacc gggatccgaa
     3121 cctgtccttg gcgggcttcc gaaatcggct cgagataccg gagggggagc ccggtcacgt
     3181 gccatggttt cagcgcatgg atatcccgga aggccagatc gggcacgtaa ataagattca
     3241 acgattcaat ttcgttcatg gcaagaattc aggaaaagtc aaattcagcg ataagacagg
     3301 acgggtaaaa cgctaccatc attccaagta taaagatgcc actaagcctt acaaatttct
     3361 tgaagaatcc aagaaagtca gtgctctgga ctccatcctt gccattatca caatcggtga
     3421 tgactgggta gtgtttgaca ttcgcggtct gtatagaaat gttttttatc gcgaactggc
     3481 acagaagggc ctgacagcag tgcagctgct ggatctgttt acgggggatc cggtgattga
     3541 cccgaagaag ggcgttgtga cattcagcta taaggaaggc gtggttccag tattttcaca
     3601 gaagatcgtt ccaaggttca agagtcgaga cacgctcgag aaattgacca gtcaaggacc
     3661 tgtggcgctg ctctcagtcg ccctcggcca aaatgaacca gtggcggcaa gggtttgtag
     3721 cttgaagaac ataaatgata agatcacatt ggataattct tgcagaatct ccttcctgga
     3781 tgactacaaa aaacaaatca aagactacag agattccctg gacgaacttg aaatcaagat
     3841 acgactggaa gcaatcaatt ctctggaaac taaccaacaa gtagaaattc gcgacctgga
     3901 tgtattcagt gctgatcggg caaaggcaaa cactgtagat atgttcgaca tcgacccaaa
     3961 tttgatatcc tgggattcaa tgagcgacgc gagggtgagc acgcaaataa gcgatcttta
     4021 tctgaagaat gggggtgacg aatctcgagt atatttcgaa attaacaaca aacggataaa
     4081 gcgatctgat tataacatta gtcagctggt gaggccaaag ctttccgaca gcactcggaa
     4141 gaatctgaac gattctatat ggaagttgaa aagaactagt gaagaatatt tgaaattgtc
     4201 caaacgaaag ttggaactga gcagagctgt tgtgaactac actatccgcc agagcaagct
     4261 cctctccgga attaacgaca ttgttataat acttgaggac ctggatgtaa aaaaaaaatt
     4321 caatggcagg ggcattcgag atatcggatg ggacaacttc ttcagctcca ggaaagagaa
     4381 caggtggttc attccggcat tccataaggc tttctcagag ctttcaagca accggggcct
     4441 ctgtgtcatc gaagtcaacc cggcatggac atctgccacc tgtcccgact gcgggttctg
     4501 tagtaaagag aacagagatg gcattaattt tacctgtcgc aagtgcggtg tctcttacca
     4561 cgcggacata gatgttgcca ctcttaatat agcccgggtg gccgttctcg gcaagcctat
     4621 gtccggaccc gccgaccgcg agagactggg cgatactaag aaaccccggg tagcaaggag
     4681 ccgaaagact atgaaacgga aagatattag caatagcacc gttgaggcta tggttacagc
     4741 caagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag attacaaaga
     4801 cgatgacgat aagggatccg atgctaagtc actgactgcc tggtcccgga cactggtgac
     4861 cttcaaggat gtgtttgtgg acttcaccag ggaggagtgg aagctgctgg acactgctca
     4921 gcagatcctg tacagaaatg tgatgctgga gaactataag aacctggttt ccttgggtta
     4981 tcagcttact aagccagatg tgatcctccg gttggagaag ggagaagagc cctggctggt
     5041 ggagagagaa attcaccaag agacccatcc tgattcagag actgcatttg aaatcaaatc
     5101 atcagttccg aaaaagaaac gcaaagttgc tagctccggc gcaacaaact tctctctgct
     5161 gaaacaagcc ggagatgtcg aagagaatcc tggaccgacc gagtacaagc ccacggtgcg
     5221 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5281 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     5341 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     5401 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     5461 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     5521 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     5581 agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     5641 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     5701 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     5761 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt taagtcgaca atcaacctct
     5821 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5881 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5941 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6001 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6061 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6121 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6181 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6241 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6301 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6361 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     6421 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6481 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     6541 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6601 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6661 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     6721 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6781 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     6841 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6901 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6961 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     7021 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7081 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7141 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7201 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7261 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7321 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     7381 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     7441 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7501 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7621 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7681 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     7741 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     7801 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     7861 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     7921 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     7981 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     8041 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     8101 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     8161 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     8221 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     8281 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     8341 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     8401 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8461 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8521 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8641 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8701 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8761 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9961 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10021 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10081 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10141 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10201 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10261 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10321 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10381 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10441 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10501 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10561 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10621 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10681 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    10741 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    10801 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    10861 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
    10921 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
    10981 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    11041 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    11101 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    11161 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    11221 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    11281 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgg
//
