LOCUS       sequence-373478-       11108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses beta-galactosidase via Phyper-spank which is optimized for
            Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear 
            localization signal (SV40) that is fused to beta-galactosidase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH
  TITLE     Engineered endosymbionts that alter mammalian cell surface marker, 
            cytokine and chemokine expression.
  JOURNAL   Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6.
  PUBMED    36042261
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-08-30"; volume: "5(1):888. doi"; pages: " 
            10.1038/s42003-022-03851-6"
COMMENT     Sequence Label: pDR111_Bgal NLS
FEATURES             Location/Qualifiers
     source          1..11108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(795..1877)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1839..1858
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(2128..5199)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
                     RPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT
                     YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQ
                     DSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
                     SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
                     RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENG
                     LLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
                     TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
                     GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPK
                     WSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI
                     KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQT
                     IEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQL
                     WLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGN
                     KRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKA
                     AGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE
                     VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYV
                     FPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGF
                     HMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     5151..5173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5165..5182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5166..5182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5200..5220)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5404..5420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(5524..5558)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(5638..5665)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5684..5770)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(9279..9867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9359..9378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10038..10829,10830..10898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10661..10680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10899..11003)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11071..11089
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgatggg atgtcacgca gaattaaata
      661 gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg gttattatta
      721 tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcaggatcc taactcacat
      781 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      841 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
      901 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
      961 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     1021 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     1081 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     1141 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     1201 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     1261 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     1321 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     1381 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     1441 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     1501 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgttttacag
     1561 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     1621 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     1681 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     1741 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     1801 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     1861 aacgttactg gtttcatcaa aatcgtctcc ctccgtttga atatttgatt gatcgtaacc
     1921 agatgaagca ctctttccac tatccctaca gtgttatggc ttgaacaatc acgaaacaat
     1981 aattggtacg tacgatcttt cagccgactc aaacatcaaa tcttacaaat gtagtctttg
     2041 aaagtattac atatgtaaga tttaaatgca accgtttttt cggaaggaaa tgatgacctc
     2101 gtttccaccg aattagcttg catgcggcta tttttgacac cagaccaact ggtaatggta
     2161 gcgaccggcg ctcagctgta attccgccga tactgacggg ctccaggagt cgtcgccacc
     2221 aatccccata tggaaaccgt cgatattcag ccatgtgcct tcttccgcgt gcagcagatg
     2281 gcgatggctg gtttccatca gttgctgttg actgtagcgg ctgatgttga actggaagtc
     2341 gccgcgccac tggtgtgggc cataattcaa ttcgcgcgtc ccgcagcgca gaccgttttc
     2401 gctcgggaag acgtacgggg tatacatgtc tgacaatggc agatcccagc ggtcaaaaca
     2461 ggcggcagta aggcggtcgg gatagttttc ttgcggccct aatccgagcc agtttacccg
     2521 ctctgctacc tgcgccagct ggcagttcag gccaatccgc gccggatgcg gtgtatcgct
     2581 cgccacttca acatcaacgg taatcgccat ttgaccacta ccatcaatcc ggtaggtttt
     2641 ccggctgata aataaggttt tcccctgatg ctgccacgcg tgagcggtcg taatcagcac
     2701 cgcatcagca agtgtatctg ccgtgcactg caacaacgct gcttcggcct ggtaatggcc
     2761 cgccgccttc cagcgttcga cccaggcgtt agggtcaatg cgggtcgctt cacttacgcc
     2821 aatgtcgtta tccagcggtg cacgggtgaa ctgatcgcgc agcggcgtca gcagttgttt
     2881 tttatcgcca atccacatct gtgaaagaaa gcctgactgg cggttaaatt gccaacgctt
     2941 attacccagc tcgatgcaaa aatccatttc gctggtggtc agatgcggga tggcgtggga
     3001 cgcggcgggg agcgtcacac tgaggttttc cgccagacgc cactgctgcc aggcgctgat
     3061 gtgcccggct tctgaccatg cggtcgcgtt cggttgcact acgcgtactg tgagccagag
     3121 ttgcccggcg ctctccggct gcggtagttc aggcagttca atcaactgtt taccttgtgg
     3181 agcgacatcc agaggcactt caccgcttgc cagcggctta ccatccagcg ccaccatcca
     3241 gtgcaggagc tcgttatcgc tatgacggaa caggtattcg ctggtcactt cgatggtttg
     3301 cccggataaa cggaactgga aaaactgctg ctggtgtttt gcttccgtca gcgctggatg
     3361 cggcgtgcgg tcggcaaaga ccagaccgtt catacagaac tggcgatcgt tcggcgtatc
     3421 gccaaaatca ccgccgtaag ccgaccacgg gttgccgttt tcatcatatt taatcagcga
     3481 ctgatccacc cagtcccaga cgaagccgcc ctgtaaacgg ggatactgac gaaacgcctg
     3541 ccagtattta gcgaaaccgc caagactgtt acccatcgcg tgggcgtatt cgcaaaggat
     3601 cagcgggcgc gtctctccag gtagcgaaag ccattttttg atggaccatt tcggcacagc
     3661 cgggaagggc tggtcttcat ccacgcgcgc gtacatcggg caaataatat cggtggccgt
     3721 ggtgtcggct ccgccgcctt catactgcac cgggcgggaa ggatcgacag atttgatcca
     3781 gcgatacagc gcgtcgtgat tagcgccgtg gcctgattca ttccccagcg accagatgat
     3841 cacactcggg tgattacgat cgcgctgcac cattcgcgtt acgcgttcgc tcatcgccgg
     3901 tagccagcgc ggatcatcgg tcagacgatt cattggcacc atgccgtggg tttcaatatt
     3961 ggcttcatcc accacataca ggccgtagcg gtcgcacagc gtgtaccaca gcggatggtt
     4021 cggataatgc gaacagcgca cggcgttaaa gttgttctgc ttcatcagca ggatatcctg
     4081 caccatcgtc tgctcatcca tgacctgacc atgcagagga tgatgctcgt gacggttaac
     4141 gcctcgaatc agcaacggct tgccgttcag cagcagcaga ccattttcaa tccgcacctc
     4201 gcggaaaccg acatcgcagg cttctgcttc aatcagcgtg ccgtcggcgg tgtgcagttc
     4261 aaccaccgca cgatagagat tcgggatttc ggcgctccac agtttcgggt tttcgacgtt
     4321 cagacgtagt gtgacgcgat cggcataacc accacgctca tcgataattt caccgccgaa
     4381 aggcgcggtg ccgctggcga cctgcgtttc accctgccat aaagaaactg ttacccgtag
     4441 gtagtcacgc aactcgccgc acatctgaac ttcagcctcc agtacagcgc ggctgaaatc
     4501 atcattaaag cgagtggcaa catggaaatc gctgatttgt gtagtcggtt tatgcagcaa
     4561 cgagacgtca cggaaaatgc cgctcatccg ccacatatcc tgatcttcca gataactgcc
     4621 gtcactccag cgcagcacca tcaccgcgag gcggttttct ccggcgcgta aaaatgcgct
     4681 caggtcaaat tcagacggca aacgactgtc ctggccgtaa ccgacccagc gcccgttgca
     4741 ccacagatga aacgccgagt taacgccatc aaaaataatt cgcgtctggc cttcctgtag
     4801 ccagctttca tcaacattaa atgtgagcga gtaacaaccc gtcggattct ccgtgggaac
     4861 aaacggcgga ttgaccgtaa tgggataggt cacgttggtg tagatgggcg catcgtaacc
     4921 gtgcatctgc cagtttgagg ggacgacgac agtatcggcc tcaggaagat cgcactccag
     4981 ccagctttcc ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag
     5041 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     5101 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     5161 acgttgtaaa acgacggcca gtgaatccgt aatcatggtc acttttcttt tttttttagg
     5221 cgctcccact gattgcgcaa ttgtcagtcc cagtgacagt cccgcaattt ttcccgctcc
     5281 ttgaataaat tttcttctgt caaatgtgtt gttttgaaat gattcttctt tcagtttttg
     5341 cacccattcg tcaaatcttg agtcatacgc cataacttac ctccttctgt cgactaagct
     5401 taattgttat ccgctcacaa ttacacacat tatgccacac cttgtagata aagtcaacaa
     5461 cttttgcaaa atgaattgtg agtgctcaca tttaccctcg agcaacgttc ttgccattgc
     5521 tgcataaaaa acgcccggcg gcaaccgagc gttctgaatt aattaatcat cgggaagatc
     5581 ttcatcaccg aaacgcggca ggcagctcta gagttaacaa gagtttgtag aaacgcaaaa
     5641 aggccatccg tcaggatggc cttctgctta gctagagcgg cggatttgtc ctactcagga
     5701 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     5761 gttttatttg atgcctcaag ctagaggtcg aattcctgca gctggcgaat ggcgattttc
     5821 gttcgtgaat acatgttata ataactataa ctaataacgt aacgtgactg gcaagagata
     5881 tttttaaaac aatgaatagg tttacactta ctttagtttt atggaaatga aagatcatat
     5941 catatataat ctagaataaa attaactaaa ataattatta tctagataaa aaatttagaa
     6001 gccaatgaaa tctataaata aactaaatta agtttattta attaacaact atggatataa
     6061 aataggtact aatcaaaata gtgaggagga tatatttgaa tacatacgaa caaattaata
     6121 aagtgaaaaa aatacttcgg aaacatttaa aaaataacct tattggtact tacatgtttg
     6181 gatcaggagt tgagagtgga ctaaaaccaa atagtgatct tgacttttta gtcgtcgtat
     6241 ctgaaccatt gacagatcaa agtaaagaaa tacttataca aaaaattaga cctatttcaa
     6301 aaaaaatagg agataaaagc aacttacgat atattgaatt aacaattatt attcagcaag
     6361 aaatggtacc gtggaatcat cctcccaaac aagaatttat ttatggagaa tggttacaag
     6421 agctttatga acaaggatac attcctcaga aggaattaaa ttcagattta accataatgc
     6481 tttaccaagc aaaacgaaaa aataaaagaa tatacggaaa ttatgactta gaggaattac
     6541 tacctgatat tccattttct gatgtgagaa gagccattat ggattcgtca gaggaattaa
     6601 tagataatta tcaggatgat gaaaccaact ctatattaac tttatgccgt atgattttaa
     6661 ctatggacac gggtaaaatc ataccaaaag atattgcggg aaatgcagtg gctgaatctt
     6721 ctccattaga acatagggag agaattttgt tagcagttcg tagttatctt ggagagaata
     6781 ttgaatggac taatgaaaat gtaaatttaa ctataaacta tttaaataac agattaaaaa
     6841 aattataaaa aaattgaaaa aatggtggaa acactttttt caattttttt gttttattat
     6901 ttaatatttg ggaaatattc attctaattg gtaatcagat tttagaaaac aataaaccct
     6961 tgcatagggg gaatccgttt aggctgggcg gtgatagctt ctcgttcagg cagtacgcct
     7021 cttttctttt ccagacctga gggaggcgga aatggtgtga ggttcccggg gaaaagccaa
     7081 ataggcgatc gcgggagtgc tttatttgaa gatcaggcta tcactgcggt caatagattt
     7141 cacaatgtga tggctggaca gcctgaggaa ctctcgaacc cgaatggaaa caaccagata
     7201 tttatgaatc agcgcggctc acatggcgtt gtgctggcaa atgcaggttc atcctctgtc
     7261 tctatcaata cggcaacaaa attgcctgat ggcaggtatg acaataaagc tggagcgggt
     7321 tcatttcaag tgaacgatgg taaactgaca ggcacgatca atgccaggtc tgtagctgtg
     7381 ctttatcctg atgatattgc aaaagcgcct catgttttcc ttgagaatta caaaacaggt
     7441 gtaacacatt ctttcaatga tcaactgacg attaccttgc gtgcagatgc gaatacaaca
     7501 aaagccgttt atcaaatcaa taatggacca gagacggcgt ttaaggatgg agatcaattc
     7561 acaatcggaa aaggagatcc atttggcaaa acatacacca tcatgttaaa aggaacgaac
     7621 agtgatggtg taacgaggac cgagaaatac agttttgtta aaagagatcc agcgtcggcc
     7681 aaaaccatcg gctatcaaaa tccgaatcat tggagccagg taaatgctta tatctataaa
     7741 catgatggga gccgagtaat tgaattgacc ggatcttggc ctggaaaacc aatgactaaa
     7801 aatgcagacg gaatttacac gctgacgctg cctgcggaca cggatacaac caacgcaaaa
     7861 gtgattttta ataatggcag cgcccaagtg cccggtcaga atcagcctgg ctttgattac
     7921 gtgctaaatg gtttatataa tgactcgggc ttaagcggtt ctcttcccca ttgagggcaa
     7981 ggctagacgg gacttaccga aagaaaccat caatgatggt ttcttttttg ttcataaatc
     8041 agacaaaact tttctcttgc aaaagtttgt gaagtgttgc acaatataaa tgtgaaatac
     8101 ttcacaaaca aaaagacatc aaagagaaac ataccctgca aggatgctga tattgtctgc
     8161 atttgcgccg gagcaaacca aaaacctggt gagacacgcc ttgaattagt agaaaagaac
     8221 ttgaagattt tcaaaggcat cgttagtgaa gtcatggcga gcggatttga cggcattttc
     8281 ttagtcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     8341 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     8401 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc
     8461 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     8521 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     8581 tactgaagtg gatttcttta agagctcctt taacttcctc accagtagtt gtatcggtac
     8641 cataagtaga agcagcaacc caagtagctt taccagcatc cggttcaacc agcatagtaa
     8701 gaatcttact ggacatcggc agttcttcga acagtgcgcc aactaccagc tctttctgca
     8761 gttcattcag ggcaccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     8821 tcctcatcct gtctcttgat ccatggatta cgcgttaacc cgggcccgcg gatgcatatg
     8881 atcagatctt aaggcctagg tctagagtct ttgttttgac gccattagcg tacgtaacaa
     8941 tcctcgttaa aggacaagga cctgagcgga agtgtatcgt acagtagacg gagtatacta
     9001 gtatagtcta tagtccgtgg aattattata tttatctccg acgatattct catcagtgaa
     9061 atccagctgg agttctttag caaatttttt tattagctga acttagtatt agtggccata
     9121 ctcctccaat ccaaagctat ttagaaagat tactatatcc tcaaacaggc ggtaaccggc
     9181 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg
     9241 aagtatccag ctcgaggtcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10321 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
    10381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10621 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
    10681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    11041 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
    11101 caagaatt
//
