LOCUS       sequence-37348-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas 
            E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM
  TITLE     mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP 
            Pathway.
  JOURNAL   Cell. 2011 Aug 5;146(3):408-20.
  PUBMED    21816276
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-08-5"; volume: "146(3)"; pages: "408-20"
COMMENT     Sequence Label: pLKO-puro FLAG SREBP1
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        243..261
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     243..260
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     274..293
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             352..375
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggggtttgc gactgttngt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
       61 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      121 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc
      181 cccttggctt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
      241 cgatttaggt gacactatag aataacatcc actttgcctt tctctccaca ggtgtccact
      301 cccaggtcca actgcacctc ggttctatcg attgaattca ccatggaaat ggactacaag
      361 gatgacgatg acaagggtgg tggtggtggt gcgtcgacga tggacgagct ggccttcggt
      421 gaggcggctc tggaacagac actggccgag atgtgcgaac tggacacagc ggttttgaac
      481 gacatcgaag acatgctcca gctcatcaac aaccaagaca gtgacttccc gggcctgttt
      541 gacgccccct atgctggggg tgagacaggg gacacaggcc ccagcagccc aggtgccaac
      601 tctcctgaga gcttctcttc tgcttctctg gcctcctctc tggaagcctt cctgggagga
      661 cccaaggtga cacctgcacc cttgtcccct ccaccatcgg cacccgctgc tttaaagatg
      721 tacccgtccg tgtccccctt ttcccctggg cctgggatca aagaggagcc agtgccactc
      781 accatcctac agcctgcagc gccacagccg tcaccgggga ccctcctgcc tccgagcttc
      841 cccgcaccac ccgtacagct cagcccttgc gcccgtgctg ggttactcga gcctgccttc
      901 aggcttctca gggacccttc caggaaaaca ctcagcagcc accatctagc ctgccgctgg
//
