LOCUS       sequence-373480-        5405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Flag-tagged iBox-PAK4cat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Li TL, Wang Z, You H, Ong Q, Varanasi VJ, Dong M, Lu B, Pasca SP, 
            Cui B
  TITLE     Engineering a Genetically Encoded Magnetic Protein Crystal.
  JOURNAL   Nano Lett. 2019 Oct 9;19(10):6955-6963. doi: 
            10.1021/acs.nanolett.9b02266. Epub 2019 Sep 25.
  PUBMED    31552740
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.9b02266"; journalName: "Nano Lett"; date: 
            "2019-10-9- 9"; volume: "19"; issue: "10"; pages: "6955-6963"
COMMENT     Sequence Label: pXJ40-Flag-iBox-PAK4cat
FEATURES             Location/Qualifiers
     source          1..5405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        313..516
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     467..487
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     513..537
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          678..1250
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1258..1277
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1305..1324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1305..1323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1338..1361
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2498..2619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2535..2554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2589..2608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2653..2670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2824..3412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2904..2923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3583..4374,4375..4443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4206..4225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4444..4548)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4616..4634
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4672..4694)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4794..4813
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4880..5335)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5017..5038)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5229..5248
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagagtccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      361 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      421 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      481 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      541 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      601 cgcggccggg aacggtgcat tggaacggac ccctaggctt ttgcaaaaag ctccggatcg
      661 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
      721 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
      781 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
      841 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
      901 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
      961 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
     1021 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
     1081 gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt
     1141 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
     1201 aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1261 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat
     1321 agggcgaatt caccatggac tacaaggacg acgatgataa gtccggatcc gaagcagagg
     1381 actggacagc agccctactg aacaggggtc gcagtcgcca gcccctggta ctaggggaca
     1441 attgctttgc tgacttggtg cacaactgga tggagctgcc tgaggaattc cccgccgccc
     1501 ctgctgttcc tgggccccct ggcccccgct caccacagcg ggagccacag cgagtatccc
     1561 atgagcagtt ccgggctgcc ctgcagctgg tggtggaccc aggcgacccc cgctcctacc
     1621 tggacaactt catcaagatt ggcgagggct ccacgggcat cgtgtgcatc gccaccgtgc
     1681 gcagctcggg caagctggtg gccgtcaaga agatggacct gcgcaagcag cagaggcgcg
     1741 agctgctctt caacgaggtg gtaatcatga gggactacca gcacgagaat gtggtggaga
     1801 tgtacaacag ctacctggtg ggggacgagc tctgggtggt catggagttc ctggaaggag
     1861 gcgccctcac cgacatcgtc acccacacca ggatgaacga ggagcagatc gcagccgtgt
     1921 gccttgcagt gctgcaggcc ctgtcggtgc tccacgccca gggcgtcatc caccgggaca
     1981 tcaagagcga ctcgatcctg ctgacccatg atggcagggt gaagctgtca gactttgggt
     2041 tctgcgccca ggtgagcaag gaagtgcccc gaaggaagtc gctggtcggc acgccctact
     2101 ggatggcccc agagctcatc tcccgccttc cctacgggcc agaggtagac atctggtcgc
     2161 tggggataat ggtgattgag atggtggacg gagagccccc ctacttcaac gagccacccc
     2221 tcaaagccat gaagatgatt cgggacaacc tgccaccccg actgaagaac ctgcacaagg
     2281 tgtcgccatc cctgaagggc ttcctggacc gcctgctggt gcgagaccct gcccagcggg
     2341 ccacggcagc cgagctgctg aagcacccat tcctggccaa ggcagggccg cctgccagca
     2401 tcgtgcccct catgcgccag aaccgcacca gatgactcga ggcggccgcc ccgggctgca
     2461 gggtaccaga tctgactgac tgatcttatt aaagcagaac ttgtttattg cagcttataa
     2521 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2581 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgactctaga
     2641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3241 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4561 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4621 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4681 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4741 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4801 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4861 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4921 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4981 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     5041 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     5101 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     5161 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     5221 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     5281 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt
     5341 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5401 gccag
//
