LOCUS       sequence-373482-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for CRISPR activation (CRISPRa) isogenic PAM testing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: miniCMV eGFP hPGK BSD
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..233
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(242..670)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(352..373)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     564..583
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    716..940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     929..946
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             972..1205
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1277..1865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1383..1394
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1792..1812
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1876..2274)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      2275..2284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2285..2795)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2348..2366)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(join(2802..3515,3516..3518,3519..3521))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2805..3515,3516..3518,3519..3521))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2847..2868)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3196..3215
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3455..3476
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3518..3527
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3545..3583)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_RNA        complement(3631..3706)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3838..3955
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4253..4297)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4482..4715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5208..5333
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5380..5560
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5578..5776)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        5778..6157
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6329..6348
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6423..6527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6528..6596,6597..7388)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6746..6765)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7559..8147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8048..8067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8301..8318
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8435..8456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8471..8476,8477..8494,8495..8501)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8509..8525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8514..8536
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8533..8549
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8533..8549
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8598..8719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8609..8628)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8663..8682
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8849..9223)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(9242..9289)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(9337..9438,1..228))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9351..9438,1..48)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9405..9424)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
       61 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      121 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
      181 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat
      241 taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      301 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      361 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      421 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      481 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
      541 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
      601 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
      661 cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata
      721 gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
      781 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
      841 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
      901 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt
      961 taaacgggcc ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     1021 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     1081 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     1141 ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc
     1201 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     1261 tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     1321 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     1381 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     1441 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     1501 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     1561 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     1621 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     1681 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     1741 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     1801 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     1861 tgattgtcga atcgattagc cctcccacac ataaccagag ggcagcaatt cacgaatccc
     1921 aactgccgtc ggctgtccat cactgtcctt cactatggct ttgatcccag gatgcagatc
     1981 gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg cccctgttct catttccgat
     2041 cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca gcaccacgag
     2101 ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga tgcggccgtc
     2161 gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc tgttgattgt
     2221 agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg ccatccatgg
     2281 tggcctgggg agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct
     2341 ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc
     2401 cacaccccgc ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc
     2461 agccgctatt ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc
     2521 cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga
     2581 accgcaagga accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa
     2641 gggtagcggc gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag
     2701 ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc
     2761 cagggctgcc ttggaaaagg cgcaacccca accccggatc ctcacttgta cagctcgtcc
     2821 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     2881 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
     2941 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     3001 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     3061 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     3121 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     3181 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     3241 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     3301 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
     3361 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     3421 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     3481 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcagc gctctagaac
     3541 cggtagctct gcttatatag gcctcccacc gtacacgcct acctcgcctc gagactgtgg
     3601 ggtggagggg acgctagcga attcaaaaaa gcaccgactc ggtgccactt tttcaagttg
     3661 ataacggact agccttattt taacttgcta tttctagctc taaaacagag acgtacaaaa
     3721 aagagcaaga agctaaaaaa gatttaaaaa ttatttttag cgcagttaat ggaacactag
     3781 tgatatcggt accttaatta accaaactgg atctctgctg tccctgtaat aaacccgaaa
     3841 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     3901 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     3961 gatgaatact gccatttgtc tgcagaattg gcgcacgcag tgccgatccg ttcactaatc
     4021 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
     4081 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     4141 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     4201 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     4261 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     4321 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     4381 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     4441 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     4501 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     4561 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     4621 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     4681 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     4741 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     4801 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     4861 tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     4921 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     4981 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     5041 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     5101 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     5161 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     5221 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     5281 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     5341 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     5401 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     5461 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     5521 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg
     5581 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     5641 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     5701 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     5761 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     5821 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     5881 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     5941 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     6001 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     6061 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     6121 attgattact attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca
     6181 tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag
     6241 ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa
     6301 gcagggagca gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6361 cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
     6421 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6481 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6541 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6601 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6661 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6721 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6781 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6841 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6901 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6961 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7021 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7081 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7141 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7201 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7261 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7321 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7381 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7441 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7501 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7561 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7621 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7681 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7741 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7801 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7861 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7921 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7981 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8041 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8101 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8161 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8221 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8281 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8341 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8401 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8461 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8521 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg
     8581 acggtataca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     8641 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     8701 aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga
     8761 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     8821 cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc
     8881 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
     8941 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
     9001 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
     9061 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
     9121 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
     9181 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag ttcctcacct
     9241 tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgatc
     9301 agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta ggcctccaaa
     9361 aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg cctctgcata
     9421 aataaaaaaa attagtca
//
