LOCUS       sequence-373488-        9805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, 
            expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses 
            Blasticidin resistance under PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Noviello G, Gjaltema RAF, Schulz EG
  TITLE     CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning 
            of endogenous gene expression.
  JOURNAL   Nat Commun. 2023 Jun 3;14(1):3225. doi: 10.1038/s41467-023-38909-4.
  PUBMED    37270532
REFERENCE   2  (bases 1 to 9805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-3"; volume: "14(1):3225. doi"; pages: " 
            10.1038/s41467-023-38909-4"
COMMENT     Sequence Label: pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin
FEATURES             Location/Qualifiers
     source          1..9805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3..704
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    719..943
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1282..1301
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1357..1755
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    1756..2344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1809..1829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2227..2238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   2527..2589
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2619..2639)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2619..2637)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2658..2674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2658..2674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2671..2693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2682..2698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2706..2712,2713..2730,2731..2736))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2751..2772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2889..2906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3060..3648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3140..3159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3819..4610,4611..4679))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4442..4461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4680..4784)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4810..5265)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4947..4968)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5159..5178
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5392..5414
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5406..5423
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5407..5423
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5433..5452
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5433..5451
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   5467..5501
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5823..6034
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6047..6315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6367..6387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6397..6717
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6769..6789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6799..9696
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             9706..9726
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9736..9762
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
       61 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      121 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      181 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
      241 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      301 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      361 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
      421 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
      481 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
      541 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
      601 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
      661 gatactgcga cctccctagc aaactggggc acaagcttaa ttagccatgg attaacgact
      721 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      781 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      841 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
      901 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcctctgg cctcgcacac
      961 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     1021 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     1081 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     1141 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     1201 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg gcgggcgccc
     1261 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     1321 tctcctcttc ctcatctccg ggcctttcga cctgcaatgg ccaagccttt gtctcaagaa
     1381 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     1441 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     1501 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     1561 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     1621 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     1681 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     1741 gtgtgggagg gctaaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1801 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1861 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     1921 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     1981 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2041 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2101 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2161 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2221 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2281 cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2341 ccgccggtac ctaaaagttt tgttacttta tagaagaaat tttgagtttt tgtttttttt
     2401 taataaataa ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta
     2461 aatataataa aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat
     2521 aaaacacatg cgtcaatttt acgcatgatt atctttaacg tacgtcacaa tatgattatc
     2581 tttctagggt taatctagta ttaatccagc ttttgttccc tttagtgagg gttaattgcg
     2641 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2701 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2761 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2821 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2881 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2941 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3001 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3061 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3121 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3181 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3241 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3301 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3361 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3421 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3481 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3541 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3601 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3661 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3721 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3781 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3841 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3901 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3961 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4021 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4081 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4141 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4201 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4261 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4321 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4381 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4441 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4501 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4561 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4621 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4681 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4741 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4801 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4861 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4921 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4981 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5041 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5101 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5161 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5221 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     5281 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     5341 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     5401 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     5461 ggctaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga aatattgctc
     5521 tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag tcgccgcttg
     5581 gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga actataacga
     5641 ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat ttaactcata
     5701 cgataattat attgttattt catgttctac ttacgtgata acttattata tatatatttt
     5761 cttgttatag atatcaacta gaatacgcgt aaggatctgc gatcgctccg gtgcccgtca
     5821 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     5881 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5941 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     6001 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     6061 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     6121 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     6181 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     6241 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     6301 gcgccgttac agatccaagc tgtgaccggc gcctactcta gagctagcga attcgccgcc
     6361 accatgccta agaaaaagag gaaggtgagc ggccgcggag ttcaggtgga aaccatctcc
     6421 ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca ctacaccggg
     6481 atgcttgaag atggaaagaa agttgattcc tcccgggaca gaaacaagcc ctttaagttt
     6541 atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg
     6601 ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca
     6661 ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa actggaaggc
     6721 ggatctggag gtagtggtgg atctggaggt tctggtggct ccatgagccc caagaagaag
     6781 agaaaggtgg aggccagcat cgaaaaaaaa aagtccttcg ccaagggcat gggcgtgaag
     6841 tccacactcg tgtccggctc caaagtgtac atgacaacct tcgccgaagg cagcgacgcc
     6901 aggctggaaa agatcgtgga gggcgacagc atcaggagcg tgaatgaggg cgaggccttc
     6961 agcgctgaaa tggccgataa aaacgccggc tataagatcg gcaacgccaa attcagccat
     7021 cctaagggct acgccgtggt ggctaacaac cctctgtata caggacccgt ccagcaggat
     7081 atgctcggcc tgaaggaaac tctggaaaag aggtacttcg gcgagagcgc tgatggcaat
     7141 gacaatattt gtatccaggt gatccataac atcctggaca ttgaaaaaat cctcgccgaa
     7201 tacattacca acgccgccta cgccgtcaac aatatctccg gcctggataa ggacattatt
     7261 ggattcggca agttctccac agtgtatacc tacgacgaat tcaaagaccc cgagcaccat
     7321 agggccgctt tcaacaataa cgataagctc atcaacgcca tcaaggccca gtatgacgag
     7381 ttcgacaact tcctcgataa ccccagactc ggctatttcg gccaggcctt tttcagcaag
     7441 gagggcagaa attacatcat caattacggc aacgaatgct atgacattct ggccctcctg
     7501 agcggactga ggcactgggt ggtccataac aacgaagaag agtccaggat ctccaggacc
     7561 tggctctaca acctcgataa gaacctcgac aacgaataca tctccaccct caactacctc
     7621 tacgacagga tcaccaatga gctgaccaac tccttctcca agaactccgc cgccaacgtg
     7681 aactatattg ccgaaactct gggaatcaac cctgccgaat tcgccgaaca atatttcaga
     7741 ttcagcatta tgaaagagca gaaaaacctc ggattcaata tcaccaagct cagggaagtg
     7801 atgctggaca ggaaggatat gtccgagatc aggaaaaatc ataaggtgtt cgactccatc
     7861 aggaccaagg tctacaccat gatggacttt gtgatttata ggtattacat cgaagaggat
     7921 gccaaggtgg ctgccgccaa taagtccctc cccgataatg agaagtccct gagcgagaag
     7981 gatatctttg tgattaacct gaggggctcc ttcaacgacg accagaagga tgccctctac
     8041 tacgatgaag ctaatagaat ttggagaaag ctcgaaaata tcatgcacaa catcaaggaa
     8101 tttaggggaa acaagacaag agagtataag aagaaggacg cccctagact gcccagaatc
     8161 ctgcccgctg gccgtgatgt ttccgccttc agcaaactca tgtatgccct gaccatgttc
     8221 ctggatggca aggagatcaa cgacctcctg accaccctga ttaataaatt cgataacatc
     8281 cagagcttcc tgaaggtgat gcctctcatc ggagtcaacg ctaagttcgt ggaggaatac
     8341 gcctttttca aagactccgc caagatcgcc gatgagctga ggctgatcaa gtccttcgct
     8401 agaatgggag aacctattgc cgatgccagg agggccatgt atatcgacgc catccgtatt
     8461 ttaggaacca acctgtccta tgatgagctc aaggccctcg ccgacacctt ttccctggac
     8521 gagaacggaa acaagctcaa gaaaggcaag cacggcatga gaaatttcat tattaataac
     8581 gtgatcagca ataaaaggtt ccactacctg atcagatacg gtgatcctgc ccacctccat
     8641 gagatcgcca aaaacgaggc cgtggtgaag ttcgtgctcg gcaggatcgc tgacatccag
     8701 aaaaaacagg gccagaacgg caagaaccag atcgacaggt actacgaaac ttgtatcgga
     8761 aaggataagg gcaagagcgt gagcgaaaag gtggacgctc tcacaaagat catcaccgga
     8821 atgaactacg accaattcga caagaaaagg agcgtcattg aggacaccgg cagggaaaac
     8881 gccgagaggg agaagtttaa aaagatcatc agcctgtacc tcaccgtgat ctaccacatc
     8941 ctcaagaata ttgtcaatat caacgccagg tacgtcatcg gattccattg cgtcgagcgt
     9001 gatgctcaac tgtacaagga gaaaggctac gacatcaatc tcaagaaact ggaagagaag
     9061 ggattcagct ccgtcaccaa gctctgcgct ggcattgatg aaactgcccc cgataagaga
     9121 aaggacgtgg aaaaggagat ggctgaaaga gccaaggaga gcattgacag cctcgagagc
     9181 gccaacccca agctgtatgc caattacatc aaatacagcg acgagaagaa agccgaggag
     9241 ttcaccaggc agattaacag ggagaaggcc aaaaccgccc tgaacgccta cctgaggaac
     9301 accaagtgga atgtgatcat cagggaggac ctcctgagaa ttgacaacaa gacatgtacc
     9361 ctgttcagaa acaaggccgt ccacctggaa gtggccaggt atgtccacgc ctatatcaac
     9421 gacattgccg aggtcaattc ctacttccaa ctgtaccatt acatcatgca gagaattatc
     9481 atgaatgaga ggtacgagaa aagcagcgga aaggtgtccg agtacttcga cgctgtgaat
     9541 gacgagaaga agtacaacga taggctcctg aaactgctgt gtgtgccttt cggctactgt
     9601 atccccaggt ttaagaacct gagcatcgag gccctgttcg ataggaacga ggccgccaag
     9661 ttcgacaagg agaaaaagaa ggtgtccggc aattccggat ccggacctaa gaaaaagagg
     9721 aaggtggcgg ccgcttaccc atacgatgtt ccagattacg ctgctagcgg cagtggaggc
     9781 ggatctggag gtagtggtgg atctg
//
