LOCUS       sequence-37349-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas 
            E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM
  TITLE     mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP 
            Pathway.
  JOURNAL   Cell. 2011 Aug 5;146(3):408-20.
  PUBMED    21816276
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-08-5"; volume: "146(3)"; pages: "408-20"
COMMENT     Sequence Label: pLKO-puro FLAG wildtype, catalytic active lipin 1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        243..261
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     243..260
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     274..293
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             352..375
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagcagagc tgttagtgaa ccgtcagaat cgcctggaga cgccatccac gctgnttttg
       61 acctccatat taagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      121 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc
      181 cccttggctt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
      241 cgatttaggt gacactatag aataacatcc actttgcctt tctctccaca ggtgtccact
      301 cccaggtcca actgcacctc ggttctatcg attgaattca ccatggaaat ggactacaag
      361 gatgacgatg acaagggtgg tggtggtggt gcgtcgacga tgaattacgt ggggcagctg
      421 gccggccagg tgtttgtgac ggtgaaggag ctctacaagg gactgaaccc cgccacgctg
      481 tcgggatgca tcgacatcat tgtcatccgg cagcccaatg gcagtctgca gtgctcccct
      541 ttccacgtcc gcttcggcaa gatgggtgtc ctccgctccc gagagaaagt ggtggacata
      601 gaaatcaatg gggagtccgt ggatttgcac atgaagttgg gagacaacgg agaagcattt
      661 tttgttcaag agacggacaa cgatcaggaa atcatcccca tgtacctggc cacgtccccc
      721 atcctgtcag aaggagctgc gagaatggaa agccagctga agaggaactc tgtggacaga
      781 atcaggtgcc tggatcccac tacagctgcc cagggcctgc ctcccagcga caccccatcc
      841 actggttctc tgggggaaga ananaaggaa aaggaggagg aaaggcccag ttggacaatc
//
