LOCUS       sequence-373491-       11972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged PlmdCas12e KRAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: PlmdCas12e KRAB
FEATURES             Location/Qualifiers
     source          1..11972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5378..5425
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5426..5449
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5483..5677
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             5744..5764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5777..5833
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5834..6430
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6327..6347
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6446..7034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6499..6519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6917..6928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7106..7339
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7365..7382)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7371..7595
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7641..8069
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7728..7747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7938..7959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8078..8098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8083..8412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8263..8398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8325..8344
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8460..8507
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8526..8900
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9030..9151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9067..9086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9121..9140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9200..9216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9200..9216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9213..9235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9224..9240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9248..9254,9255..9272,9273..9278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9293..9314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9431..9448)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9602..10190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9682..9701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10361..11152,11153..11221))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10984..11003
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11222..11326)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11401..11420)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11592..11971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatgcaag agatcaagag
     2461 aatcaacaag atcagaagga gactggtcaa ggacagcaac acaaagaagg ccggcaagac
     2521 aggccccatg aaaaccctgc tcgtcagagt gatgacccct gacctgagag agcggctgga
     2581 aaacctgaga aagaagcccg agaacatccc tcagcctatc agcaacacca gcagggccaa
     2641 cctgaacaag ctgctgaccg actacaccga gatgaagaaa gccatcctgc acgtgtactg
     2701 ggaagagttc cagaaagacc ccgtgggcct gatgagcaga gttgctcagc ccgctcctaa
     2761 gaacatcgac cagagaaagc tgatccccgt gaaggacggc aacgagagac tgacctctag
     2821 cggctttgcc tgcagccagt gttgccagcc tctgtacgtg tacaagctgg aacaagtgaa
     2881 cgacaagggc aagccccaca ccaactactt cggcagatgc aacgtgtccg agcacgagag
     2941 gctgatcctg ctgtctcctc acaagcccga ggccaacgat gagctggtca catacagcct
     3001 gggcaagttc ggacagagag ccctggactt ctacagcatc cacgtgacca gggagagcaa
     3061 tcaccctgtg aagcccctgg aacagatcgg cggcaatagc tgtgcctctg gacctgtggg
     3121 aaaagccctg agcgacgcct gtatgggagc cgtggcatcc ttcctgacca agtaccagga
     3181 catcatcctg gaacaccaga aagtgatcaa gaagaacgag aaaagactgg ccaacctcaa
     3241 ggatatcgcc agcgctaacg gcctggcctt tcctaagatc accctgcctc cacagcctca
     3301 caccaaagag ggcatcgagg cctacaacaa cgtggtggcc cagatcgtga tttgggtcaa
     3361 cctgaatctg tggcagaagc tgaagatcgg cagggacgaa gccaagccac tgcagagact
     3421 gaagggcttc cctagcttcc ctctggtgga aagacaggcc aatgaagtgg attggtggga
     3481 catggtctgc aacgtgaaga agctgatcaa cgagaagaaa gaggatggca aggttttctg
     3541 gcagaacctg gccggctaca agagacaaga agccctgctg ccttacctga gcagcgaaga
     3601 ggaccggaag aagggcaaga agttcgccag ataccagttc ggcgacctgc tgctgcacct
     3661 ggaaaagaag cacggcgagg actggggcaa agtgtacgat gaggcctggg agagaatcga
     3721 caagaaggtg gaaggcctga gcaagcacat taagctggaa gaggaaagaa ggagcgagga
     3781 cgcccaatct aaagccgctc tgaccgattg gctgagagcc aaggccagct ttgtgatcga
     3841 gggcctgaaa gaggccgaca aggacgagtt ctgcagatgc gagctgaagc tgcagaagtg
     3901 gtacggcgat ctgagaggca agcccttcgc cattgaggcc gagaacagca tcctggacat
     3961 cagcggcttc agcaagcagt acaactgcgc cttcatttgg cagaaagacg gcgtcaagaa
     4021 actgaacctg tacctgatca tcaattactt caaaggcggc aagctgcggt tcaagaagat
     4081 caaacccgag gccttcgagg ctaacagatt ctacaccgtg atcaacaaaa agtccggcga
     4141 gatcgtgccc atggaagtga acttcaactt cgacgacccc aacctgatta tcctgcctct
     4201 ggccttcggc aagagacagg gcagagagtt catctggaac gatctgctga gcctggaaac
     4261 cggctctctg aagctggcca atggcagagt gatcgagaaa accctgtaca acaggagaac
     4321 cagacaggac gagcctgctc tgtttgtggc cctgaccttc gagagaagag aggtgctgga
     4381 cagcagcaac atcaagccca tgaacctgat cggcatcgcc cggggcgaga atatccctgc
     4441 tgtgatcgcc ctgacagacc ctgaaggatg cccactgagc agattcaagg actccctggg
     4501 caaccctaca cacatcctga gaatcggcga gagctacaaa gagaagcaga ggacaatcca
     4561 ggccgccaaa gaggtggaac agagaagagc cggcggatac tctaggaagt acgccagcaa
     4621 ggccaagaat ctggccgacg acatggtccg aaacaccgcc agagatctgc tgtactacgc
     4681 cgtgacacag gacgccatgc tgatcttcgc caatctgagc agaggcttcg gccggcaggg
     4741 caagagaacc tttatggccg agaggcagta caccagaatg gaagattggc tcacagctaa
     4801 actggcctac gagggactgc ccagcaagac ctacctgtcc aaaacactgg cccagtatac
     4861 ctccaagacc tgcagcaatt gcggcttcac catcaccagc gccgactacg acagagtgct
     4921 ggaaaagctc aagaaaaccg ccaccggctg gatgaccacc atcaacggca aagagctgaa
     4981 ggttgagggc cagatcacct actacaacag gtacaagagg cagaacgtcg tgaaggatct
     5041 gagcgtggaa ctggacagac tgagcgaaga gagcgtgaac aacgacatca gcagctggac
     5101 aaagggcaga tcaggcgagg ctctgagcct gctgaagaag aggtttagcc acagacctgt
     5161 gcaagagaag ttcgtgtgcc tgaactgcgg cttcgagaca cacgccgccg aacaggctgc
     5221 cctgaacatt gccagaagct ggctgttcct gagaagccaa gagtacaaga agtaccagac
     5281 caacaagacc accggcaaca ccgacaagag ggcctttgtg gaaacctggc agagcttcta
     5341 cagaaaaaag ctgaaagaag tctggaagcc cgccgtgaag cgacctgccg ccacaaagaa
     5401 ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg gatccgatgc
     5461 taagtcactg actgcctggt cccggacact ggtgaccttc aaggatgtgt ttgtggactt
     5521 caccagggag gagtggaagc tgctggacac tgctcagcag atcctgtaca gaaatgtgat
     5581 gctggagaac tataagaacc tggtttcctt gggttatcag cttactaagc cagatgtgat
     5641 cctccggttg gagaagggag aagagccctg gctggtggag agagaaattc accaagagac
     5701 ccatcctgat tcagagactg catttgaaat caaatcatca gttccgaaaa agaaacgcaa
     5761 agttgctagc tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga
     5821 gaatcctgga ccgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5881 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5941 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6001 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6061 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6121 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6181 gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa
     6241 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6301 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6361 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6421 cggtgcctga acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6481 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6541 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6601 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6661 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6721 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6781 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6841 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6901 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6961 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7021 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     7081 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7141 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7201 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7261 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7321 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     7381 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7441 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7501 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7561 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     7621 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7681 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7741 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7801 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7861 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7921 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7981 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8041 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     8101 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8161 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8221 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8341 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8401 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     8461 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     8521 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     8581 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     8641 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     8701 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     8761 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     8821 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     8881 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     8941 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9001 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     9061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9121 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     9181 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9241 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9301 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9361 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9421 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9481 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9541 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9601 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9661 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9721 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9781 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9901 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9961 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10021 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10081 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10141 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10201 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10261 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10321 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10501 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11341 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
    11401 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    11461 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
    11521 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
    11581 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    11641 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    11701 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    11761 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    11821 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    11881 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    11941 catctacgta ttagtcatcg ctattaccat gg
//
