LOCUS       sequence-373492-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Dalla Benetta E, Lopez-Denman AJ, Li HH, Masri RA, Brogan DJ, Bui M,
            Yang T, Li M, Dunn M, Klein MJ, Jackson S, Catalan K, Blasdell KR, 
            Tng P, Antoshechkin I, Alphey LS, Paradkar PN, Akbari OS
  TITLE     Engineered Antiviral Sensor Targets Infected Mosquitoes.
  JOURNAL   CRISPR J. 2023 Dec;6(6):543-556. doi: 10.1089/crispr.2023.0056.
  PUBMED    38108518
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2023"; journalName: "CRISPR J"; date: "2023-12"; 
            volume: "6"; issue: "6"; pages: "543-556"
COMMENT     Sequence Label: OA-1085L
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_RNA        5068..5103
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5134..5169
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5200..5235
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5266..5301
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        5332..5367
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     CDS             join(6525..6527,6528..6530,6531..7244)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6570..6591)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6831..6850)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7178..7199
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7279..7360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      7741..7750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7880..7898)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8148..8167
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8342..8365
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9299..9420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   10088..10122
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10304..10320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10304..10320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10317..10339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10328..10344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10352..10358,10359..10376,10377..10382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10397..10418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10535..10552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccgggctcct aggagatctt
     4081 catcacaaac gaaactatta taatcgtctt caggggaacg gtagcatcat atctgtcacc
     4141 agtcagtcgg attcctttct ttcggcataa attatgctcg ggtgacgaga cgagttgaaa
     4201 atcccgtaaa ttaaaacata taccacactc gccgtcctag acgacgacga cgacgacgat
     4261 tcccaaggaa ttgaaccctt catttgcatt tcactaccgc tctgagagat gcaaagcgcg
     4321 ccagacagtc gatcgaatct gcagaccaga gagtcacatc aagaatgttg acattccacc
     4381 agaagactct aattattctt cgctcgactg ggaaaactgt taacaactgc tcgagtgtgg
     4441 tcggagcggc tcacagtgcg ttttcccgta gacaaaaatc aaatttcatg gtatttttcg
     4501 gcgataaaca gtaagttaaa gggaacccga atttagtgct atataattta attccactag
     4561 agtttgtatc ctttgataga tacgcgtatt tcgacctcaa ctgcaaggcc gtcgtgtact
     4621 agacttgact aatccagact ggtcttttag ttatgacttc tgtccacatc tccatacatt
     4681 caacgcactg tgcggctgtg ctgtgcgact ccgtcgagtc gaccaacata gttgaaacaa
     4741 attgaatatt taattgatcg ttataggaat ggtgttagat gagtcatcct ttacagtaag
     4801 cacatacagt attataattg aagatcgtcg gcagataggt gtgtagggta gagtatcagc
     4861 aataagttgg gacgtttgac tttttgtagg tagacaaaaa ctaaactttt tttcgcttct
     4921 ctatgtgtgc ccctgggtag cgttccgttc cgattggggt gcgaacgaat gaaatcgccc
     4981 atcgagttga tacgtccatc catcgctaga accgcgttcg ctgtagaaga ctatataaga
     5041 gcagaggcaa gagtagtgaa atgctagcaa gtaaacccct accaactggt cggggtttga
     5101 aacgcagatg aacttcaggg tcagcttgcc gtacaagtaa acccctacca actggtcggg
     5161 gtttgaaact catgtggtcg gggtagcggc tgaagcactc aagtaaaccc ctaccaactg
     5221 gtcggggttt gaaacactcc agcttgtgcc ccaggatgtt gccgtcaagt aaacccctac
     5281 caactggtcg gggtttgaaa ctcaccttga tgccgttctt ctgcttgtcg gcaagtaaac
     5341 ccctaccaac tggtcggggt ttgaaacttt ttttggtggt tgttcatcga ttcctaatat
     5401 tgcagtgaag cagtttgttg tgcatggcga agataaggtt agggcgagga atgccttgga
     5461 aataagcggc actgggccgt atgaataaaa agcgttgaac tttactacat gtagcatcta
     5521 ctcaaaaaca aaatccacaa agtttactac acttttggta tgaataaaaa ccttttcgaa
     5581 catgtagtac ctactacttt aagaaaattc tattcaaagt acagagtaat gctgcatgtt
     5641 aagaaaattc cattcagagt gactcttgaa atcaataaaa tcatgcatat atgccaaatt
     5701 tgtcacttat tcacgtgaca aattatgtga aaatatgaat attactacat tcccgctatg
     5761 catcgcgtaa tcgatcatga aatttatgtt ggttatgtaa ctttacagta ttccctttat
     5821 gtgcatgtga attttataat gatttgacaa caaggatttt ccttagaatt ttcgaatttt
     5881 agctagaaat ctgtatccta tgaatgaatc ttgagataac agcaattgta acattataaa
     5941 gttcctaaca ttttcaagat tccagtattg aggccttttg ggccatactc ggtggcctcc
     6001 ccaccaccaa cttttttgca ctgcaaaaaa acacgctttt gcacgcgggc ccatacatag
     6061 tacaaactct acgtttcgta gactatttta cataaatagt ctacaccgtt gtatacgctc
     6121 caaatacact accacacatt gaaccttttt gcagtgcaaa aaagtacgtg tcggcagtca
     6181 cgtaggccgg ccttatcggg tcgcgtcctg tcacgtacga atcacattat cggaccggac
     6241 gagtgttgtc ttatcgtgac aggacgccag cttcctgtgt tgctaaccgc agccggacgc
     6301 aactccttat cggaacagga cgcgcctcca tatcagccgc gcgttatctc atgcgcgtga
     6361 ccggacacga ggcgcccgtc ccgcttatcg cgcctataaa tacagcccgc aacgatctgg
     6421 taaacacagt tgaacagcat ctgttacagc gacacaacat gagccggtcc aacaacgcca
     6481 acgcgcccac gccatccaac cgccgccgca acctgtctct ggtgatggtg agcaagggcg
     6541 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     6601 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     6661 agttcatctg caccaccagc aagctgcccg tgccctggcc caccctcgtg accaccctga
     6721 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     6781 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     6841 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     6901 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     6961 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     7021 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     7081 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     7141 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     7201 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaacattg atgagtttgg
     7261 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     7321 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     7381 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     7441 caaatgtggt atggctgatt atgatcagtc gaccggcgcg ccaattcgag ctcgcccggg
     7501 gatctaattc aattagagac taattcaatt agagctaatt caattaggat ccaagcttat
     7561 cgatttcgaa ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     7621 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     7681 gcagctgaac aagctaaaca atcggggtac cgctagagtc gacggtacga tccaccggtc
     7741 gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     7801 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     7861 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     7921 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     7981 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     8041 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     8101 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     8161 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     8221 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     8281 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     8341 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     8401 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
     8461 ggcaccgcct cctccgagga caacaacatg gccgttatca aggaatttat gcgcttcaaa
     8521 gttaggatgg agggatccat gaacggacat gagttcgaga tcgagggaga gggcgaggga
     8581 cgcccgtatg aaggcacaca aacagccaaa ctcaaggtca ccaagggcgg accactgccc
     8641 ttcgcctggg atatcctgag tccccagttt atgtacggca gcaaggccta cgttaagcac
     8701 cccgctgaca taccggacta caaaaagctg tcctttccgg aaggcttcaa gtgggagcgc
     8761 gtgatgaatt tcgaagacgg aggactggtc actgtgaccc aagatagcag tttgcaggac
     8821 ggtacactga tctataaggt taaaatgcgc ggcactaact ttccgccaga tggcccagtg
     8881 atgcagaaga agaccatggg ttgggaggca tccaccgaac gtctgtaccc tcgagacgga
     8941 gtgctcaagg gcgagatcca tcaggccctc aaactgaaag atggtggtca ctacctggtc
     9001 gaatttaaga ccatttacat ggccaagaag ccggttcagc tgcccggata ttattatgtg
     9061 gatacgaaac tggatataac ttcgcataac gaagactaca ccattgtcga gcagtatgag
     9121 cgcagcgaag gccgacatca cctgttcctc tacggcatgg acgagctgta caagtaggcg
     9181 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     9241 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     9301 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9361 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9421 aagcttctag gccggccgat ctcggatctg acaatgttca gtgcagagac tcggctacgc
     9481 ctcgtggact ttgaagttga ccaacaatgt ttattcttac ctctaatagt cctctgtggc
     9541 aaggtcaaga ttctgttaga agccaatgaa gaacctggtt gttcaataac attttgttcg
     9601 tctaatattt cactaccgct tgacgttggc tgcacttcat gtacctcatc tataaacgct
     9661 tcttctgtat cgctctggac gtcatcttca cttacgtgat ctgatatttc actgtcagaa
     9721 tcctcaccaa caagctcgtc atcgctttgc agaagagcag agaggatatg ctcatcgtct
     9781 aaagaactac ccattttatt atatattagt cacgatatct ataacaagaa aatatatata
     9841 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
     9901 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
     9961 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
    10021 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
    10081 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaaaaaa gatttgcgct
    10141 ttactcgacc taaactttaa acacgtcata gaatcttcgt ttgacaaaaa ccacattgtg
    10201 gccaagctgt gtgacgcgac gcgcgctaaa gaatggcaaa ccaagtcgcg cgagcgtcga
    10261 ctctagagga tccccgggta ccgagctcga attcgtaatc atggtcatag ctgtttcctg
    10321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    10381 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    10441 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    10501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg t
//
