LOCUS       sequence-373494-       13544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged SadCas9 VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: SadCas9 VPR
FEATURES             Location/Qualifiers
     source          1..13544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5603..5650
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5651..5674
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    5717..5741
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5771..5920
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5945..5965
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6395..6751
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6770..7339
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             7349..7405
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7406..8002
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7899..7919
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8018..8606
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8071..8091)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8489..8500)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8678..8911
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8937..8954)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8943..9167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9213..9641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9300..9319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9510..9531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9650..9670)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9655..9984
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9835..9970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9897..9916
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10032..10079
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10098..10472
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10602..10723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10639..10658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10693..10712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10772..10788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10772..10788)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10785..10807)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10796..10812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10820..10826,10827..10844,10845..10850))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10865..10886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11003..11020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11174..11762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11254..11273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11933..12724,12725..12793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12556..12575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12794..12898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12973..12992)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        13164..13543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatgaagc ggaactacat
     2461 cctgggcctg gccatcggca tcaccagcgt gggctacggc atcatcgact acgagacacg
     2521 ggacgtgatc gatgccggcg tgcggctgtt caaagaggcc aacgtggaaa acaacgaggg
     2581 taggcggagc aagagaggcg ccagaaggct gaagcggcgg aggcggcata gaatccagag
     2641 agtgaagaag ctgctgttcg actacaacct gctgaccgac cacagcgagc tgagcggcat
     2701 caacccctac gaggccagag tgaagggcct gagccagaag ctgagcgagg aagagttctc
     2761 tgccgccctg ctgcacctgg ccaagagaag aggcgtgcac aacgtgaacg aggtggaaga
     2821 ggacaccggc aacgagctgt ccaccaaaga gcagatcagc cggaacagca aggccctgga
     2881 agagaaatac gtggccgaac tgcagctgga acggctgaag aaagacggcg aagtgcgggg
     2941 cagcatcaac agattcaaga ccagcgacta cgtgaaagaa gccaaacagc tgctgaaggt
     3001 gcagaaggcc taccaccagc tggaccagag cttcatcgac acctacatcg acctgctgga
     3061 aacccggcgg acctactatg agggacctgg cgagggcagc cccttcggct ggaaggacat
     3121 caaagaatgg tacgagatgc tgatgggcca ctgcacctac ttccccgagg aactgcggag
     3181 cgtgaagtac gcctacaacg ccgacctgta caacgccctg aacgacctga acaatctcgt
     3241 gatcaccagg gacgagaacg agaagctgga atattacgag aagttccaga tcatcgagaa
     3301 cgtgttcaag cagaagaaga agcccaccct gaagcagatc gccaaagaaa tcctcgtgaa
     3361 cgaagaggat attaagggct acagagtgac cagcaccggc aagcccgagt tcaccaacct
     3421 gaaggtgtac cacgacatca aggacattac cgcccggaaa gagattattg agaacgccga
     3481 gctgctggat cagattgcca agatcctgac catctaccag agcagcgagg acatccagga
     3541 agaactgacc aatctgaact ccgagctgac ccaggaagag atcgagcaga tctctaatct
     3601 gaagggctat accggcaccc acaacctgag cctgaaggcc atcaacctga tcctggacga
     3661 gctgtggcac accaacgaca accagatcgc tatcttcaac cggctgaagc tggtgcccaa
     3721 gaaggtggac ctgtcccagc agaaagagat ccccaccacc ctggtggacg acttcatcct
     3781 gagccccgtc gtgaagagaa gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa
     3841 gaagtacggc ctgcccaacg acatcattat cgagctggcc cgcgagaaga actccaagga
     3901 cgcccagaaa atgatcaacg agatgcagaa gcggaaccgg cagaccaacg agcggatcga
     3961 ggaaatcatc cggaccaccg gcaaagagaa cgccaagtac ctgatcgaga agatcaagct
     4021 gcacgacatg caggaaggca agtgcctgta cagcctggaa gccatccctc tggaagatct
     4081 gctgaacaac cccttcaact atgaggtgga ccacatcatc cccagaagcg tgtccttcga
     4141 caacagcttc aacaacaagg tgctcgtgaa gcaggaagaa gccagcaaga agggcaaccg
     4201 gaccccattc cagtacctga gcagcagcga cagcaagatc agctacgaaa ccttcaagaa
     4261 gcacatcctg aatctggcca agggcaaggg cagaatcagc aagaccaaga aagagtatct
     4321 gctggaagaa cgggacatca acaggttctc cgtgcagaaa gacttcatca accggaacct
     4381 ggtggatacc agatacgcca ccagaggcct gatgaacctg ctgcggagct acttcagagt
     4441 gaacaacctg gacgtgaaag tgaagtccat caatggcggc ttcaccagct ttctgcggcg
     4501 gaagtggaag tttaagaaag agcggaacaa ggggtacaag caccacgccg aggacgccct
     4561 gatcattgcc aacgccgatt tcatcttcaa agagtggaag aaactggaca aggccaaaaa
     4621 agtgatggaa aaccagatgt tcgaggaaaa gcaggccgag agcatgcccg agatcgaaac
     4681 cgagcaggag tacaaagaga tcttcatcac cccccaccag atcaagcaca ttaaggactt
     4741 caaggactac aagtacagcc accgggtgga caagaagcct aatagagagc tgattaacga
     4801 caccctgtac tccacccgga aggacgacaa gggcaacacc ctgatcgtga acaatctgaa
     4861 cggcctgtac gacaaggaca atgacaagct gaaaaagctg atcaacaaga gccccgaaaa
     4921 gctgctgatg taccaccacg acccccagac ctaccagaaa ctgaagctga ttatggaaca
     4981 gtacggcgac gagaagaatc ccctgtacaa gtactacgag gaaaccggga actacctgac
     5041 caagtactcc aaaaaggaca acggccccgt gatcaagaag attaagtatt acggcaacaa
     5101 actgaacgcc catctggaca tcaccgacga ctaccccaac agcagaaaca aggtcgtgaa
     5161 gctgtccctg aagccctaca gattcgacgt gtacctggac aatggcgtgt acaagttcgt
     5221 gaccgtgaag aatctggatg tgatcaaaaa agaaaactac tacgaagtga atagcaagtg
     5281 ctatgaggaa gctaagaagc tgaagaagat cagcaaccag gccgagttta tcgcctcctt
     5341 ctacaacaac gatctgatca agatcaacgg cgagctgtat agagtgatcg gcgtgaacaa
     5401 cgacctgctg aaccggatcg aagtgaacat gatcgacatc acctaccgcg agtacctgga
     5461 aaacatgaac gacaagaggc cccccaggat cattaagaca atcgcctcca agacccagag
     5521 cattaagaag tacagcacag acattctggg caacctgtat gaagtgaaat ctaagaagca
     5581 ccctcagatc atcaaaaagg gcaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     5641 aaagaaaaag gattacaaag acgatgacga taagggatcc tcgccaggga tccgtcgact
     5701 tgacgcgttg atatcaacaa gtttgtacaa aaaagcaggc tacaaagagg ccagcggttc
     5761 cggacgggct gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga
     5821 tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct
     5881 cggcagtgac gcccttgatg atttcgacct ggacatgctg attaactcta gaagttccgg
     5941 atctccgaaa aagaaacgca aagttggtag ccagtacctg cccgacaccg acgaccggca
     6001 ccggatcgag gaaaagcgga agcggaccta cgagacattc aagagcatca tgaagaagtc
     6061 ccccttcagc ggccccaccg accctagacc tccacctaga agaatcgccg tgcccagcag
     6121 atccagcgcc agcgtgccaa aacctgcccc ccagccttac cccttcacca gcagcctgag
     6181 caccatcaac tacgacgagt tccctaccat ggtgttcccc agcggccaga tctctcaggc
     6241 ctctgctctg gctccagccc ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc
     6301 tccagccatg gtgtctgcac tggctcaggc accagcaccc gtgcctgtgc tggctcctgg
     6361 acctccacag gctgtggctc caccagcccc taaacctaca caggccggcg agggcacact
     6421 gtctgaagct ctgctgcagc tgcagttcga cgacgaggat ctgggagccc tgctgggaaa
     6481 cagcaccgat cctgccgtgt tcaccgacct ggccagcgtg gacaacagcg agttccagca
     6541 gctgctgaac cagggcatcc ctgtggcccc tcacaccacc gagcccatgc tgatggaata
     6601 ccccgaggcc atcacccggc tcgtgacagg cgctcagagg cctcctgatc cagctcctgc
     6661 ccctctggga gcaccaggcc tgcctaatgg actgctgtct ggcgacgagg acttcagctc
     6721 tatcgccgat atggatttct cagccttgct gggctctggc agcggcagcc gggattccag
     6781 ggaagggatg tttttgccga agcctgaggc cggctccgct attagtgacg tgtttgaggg
     6841 ccgcgaggtg tgccagccaa aacgaatccg gccatttcat cctccaggaa gtccatgggc
     6901 caaccgccca ctccccgcca gcctcgcacc aacaccaacc ggtccagtac atgagccagt
     6961 cgggtcactg accccggcac cagtccctca gccactggat ccagcgcccg cagtgactcc
     7021 cgaggccagt cacctgttgg aggatcccga tgaagagacg agccaggctg tcaaagccct
     7081 tcgggagatg gccgatactg tgattcccca gaaggaagag gctgcaatct gtggccaaat
     7141 ggacctttcc catccgcccc caaggggcca tctggatgag ctgacaacca cacttgagtc
     7201 catgaccgag gatctgaacc tggactcacc cctgaccccg gaattgaacg agattctgga
     7261 taccttcctg aacgacgagt gcctcttgca tgccatgcat atcagcacag gactgtccat
     7321 cttcgacaca tctctgtttg gatccggcgc aacaaacttc tctctgctga aacaagccgg
     7381 agatgtcgaa gagaatcctg gaccgaccga gtacaagccc acggtgcgcc tcgccacccg
     7441 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7501 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7561 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7621 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7681 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7741 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag tctcgcccga
     7801 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7861 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     7921 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     7981 gacccgcaag cccggtgcct gaacgcgtta agtcgacaat caacctctgg attacaaaat
     8041 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8101 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8161 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8221 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8281 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8341 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8401 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8461 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8521 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8581 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     8641 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8701 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8761 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8821 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8881 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     8941 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     9001 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     9061 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     9121 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     9181 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     9241 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9301 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9361 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9421 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9481 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9541 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9601 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     9661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9901 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     9961 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
    10021 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
    10081 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
    10141 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
    10201 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
    10261 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
    10321 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
    10381 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
    10441 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
    10501 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    10561 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    10621 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10681 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    10741 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10801 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10861 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10921 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10981 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    11041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    11101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11341 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11581 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    11641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11821 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11881 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11941 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    12001 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    12061 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    12121 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    12181 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    12241 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12301 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12361 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12421 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12481 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12541 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12601 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12661 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12721 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12781 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12841 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12901 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    12961 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    13021 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    13081 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
    13141 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
    13201 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    13261 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    13321 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    13381 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    13441 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    13501 tacttggcag tacatctacg tattagtcat cgctattacc atgg
//
