LOCUS       sequence-373496-       11996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged DpbdCas12e KRAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: DpbdCas12e KRAB
FEATURES             Location/Qualifiers
     source          1..11996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2438..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5402..5449
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5450..5473
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5507..5701
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             5768..5788
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5801..5857
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5858..6454
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6351..6371
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6470..7058
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6523..6543)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6941..6952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7130..7363
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7389..7406)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7395..7619
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7665..8093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7752..7771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7962..7983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8102..8122)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8107..8436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8287..8422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8349..8368
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8484..8531
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8550..8924
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9054..9175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9091..9110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9145..9164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9224..9240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9224..9240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9237..9259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9248..9264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9272..9278,9279..9296,9297..9302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9317..9338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9455..9472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9626..10214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9706..9725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10385..11176,11177..11245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11008..11027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11246..11350)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11425..11444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11616..11995
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaaa agagaatcaa
     2461 caagatcagg aagaagctga gcgccgacaa cgccaccaag cctgtgtcta ggagtggccc
     2521 catgaaaacc ctgctcgtca gagtgatgac cgacgacctg aagaagagac tggaaaagag
     2581 gaggaagaaa cccgaagtga tgccccaagt gatcagcaac aacgccgcca acaacctgag
     2641 aatgctgctg gacgactaca ccaagatgaa ggaagccatc ctgcaggtct actggcaaga
     2701 gttcaaggac gaccacgtgg gcctgatgtg caagtttgcc cagcctgcca gcaagaagat
     2761 cgaccagaac aagctgaagc ccgagatgga cgagaagggc aatctgacca cagccggctt
     2821 tgcctgctct cagtgtggcc agcctctgtt cgtgtacaag ctggaacagg tgtccgagaa
     2881 aggcaaggcc tacaccaact acttcggcag atgtaacgtg gccgagcacg agaagctgat
     2941 tctgctggcc cagctgaaac ctgagaagga ctctgatgag gccgtgacct acagcctggg
     3001 caagtttgga cagagagccc tggacttcta cagcatccac gtgaccaaag aaagcacaca
     3061 ccccgtgaag cccctggctc agatcgccgg caatagatac gcctctggac ctgtgggcaa
     3121 agccctgtcc gatgcctgca tgggaacaat cgccagcttc ctgagcaagt accaggacat
     3181 catcatcgag caccagaagg tggtcaaggg caaccagaag agactggaaa gcctgaggga
     3241 gctggccggc aaagagaacc tggaataccc cagcgtgacc ctgcctcctc agcctcacac
     3301 aaaagaaggc gtggacgcct acaacgaagt gatcgccaga gtgagaatgt gggtcaacct
     3361 gaacctgtgg cagaagctga aactgtccag ggacgacgcc aagcctctgc tgagactgaa
     3421 gggcttccct agcttccccg tggtggaaag aagggagaat gaggtggact ggtggaacac
     3481 catcaacgaa gtcaagaaac tgatcgatgc caagagagac atgggcagag tgttttggag
     3541 cggagtgacc gccgagaaga gaaacaccat cctggaaggc tacaactacc tgcctaacga
     3601 gaacgaccac aagaagagag agggcagcct ggaaaaccct aagaagcccg ccaagagaca
     3661 gttcggcgac ctgctgctgt acctggagaa gaagtatgcc ggcgactggg gcaaagtgtt
     3721 cgatgaggcc tgggagagaa tcgacaagaa gattgccggc ctgaccagcc acatcgagag
     3781 agaggaagcc agaaacgccg aggacgccca gtctaaagcc gtgctgacag attggctgag
     3841 agccaaggcc agcttcgtgc tggaaaggct gaaagaaatg gatgagaaag agttctacgc
     3901 ctgcgagatc cagctgcaga agtggtacgg ggacctgaga ggcaatccct ttgccgtgga
     3961 agccgagaac agagtggtgg acatcagcgg ctttagcatc ggctctgacg gccactccat
     4021 ccagtacagg aacctgctgg cctggaagta cctggaaaat ggcaagagag agttctacct
     4081 gctgatgaac tacggcaaga agggcagaat cagattcacc gacggcaccg acatcaagaa
     4141 aagcggcaag tggcagggcc tgctgtatgg cggcggaaag gccaaagtga tcgacctgac
     4201 cttcgatccc gacgacgagc agctgatcat tctgcctctg gccttcggca ccagacaggg
     4261 cagagagttc atctggaacg atctgctgtc cctggaaacc ggcctgatca agctggccaa
     4321 cggcagggtg atcgagaaaa ccatctacaa caagaaaatc ggcagagatg agcccgcact
     4381 gtttgtggcc ctgacctttg agaggagaga agtggtcgat cccagcaaca tcaagcccgt
     4441 gaacctgatc ggagtggcca gaggcgagaa tatccctgcc gtgattgccc tgacagaccc
     4501 tgagggatgt cccctgcctg agtttaagga tagctctggc ggccctaccg acatcctgag
     4561 aatcggcgag ggctacaaag agaagcagag agccatccag gccgccaaag aggtggaaca
     4621 gagaagagcc ggcggataca gcagaaagtt cgccagcaag tccagaaacc tggccgatga
     4681 catggtcagg aacagcgcca gggacctgtt ctaccacgcc gtgacacatg atgccgtgct
     4741 ggtgttcgcc aacctgtcaa gaggcttcgg cagacaggga aaaagaacct tcatgaccga
     4801 gagacagtac acgaagatgg aagattggct caccgccaaa ctggcctacg agggcctgac
     4861 aagcaagacc tacctgtcca agacactggc ccagtacacc tccaagacct gctccaattg
     4921 cggcttcacc atcaccaccg ccgactacga tggaatgctc gtgagactga agaaaacctc
     4981 cgacggctgg gccaccacac tgaacaacaa agagctgaaa gccgagggcc agatcaccta
     5041 ctacaacaga tacaagaggc agaccgtcga gaaagagctg tccgccgaac tcgacagact
     5101 gagcgaggaa tccggcaaca acgacatcag caagtggacc aagggacgca gagatgaggc
     5161 cctgttcctg ctgaagaaga ggttcagcca cagacctgtg caagagcagt ttgtgtgcct
     5221 ggattgcggc cacgaagtgc acgcagccga acaggccgct ctgaatatcg ccagaagctg
     5281 gctgttcctg aacagcaaca gcaccgagtt caagagctac aagagcggca agcagccttt
     5341 cgttggagcc tggcaggcct tctacaagag aaggctcaaa gaagtgtgga agcccaacgc
     5401 caagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag attacaaaga
     5461 cgatgacgat aagggatccg atgctaagtc actgactgcc tggtcccgga cactggtgac
     5521 cttcaaggat gtgtttgtgg acttcaccag ggaggagtgg aagctgctgg acactgctca
     5581 gcagatcctg tacagaaatg tgatgctgga gaactataag aacctggttt ccttgggtta
     5641 tcagcttact aagccagatg tgatcctccg gttggagaag ggagaagagc cctggctggt
     5701 ggagagagaa attcaccaag agacccatcc tgattcagag actgcatttg aaatcaaatc
     5761 atcagttccg aaaaagaaac gcaaagttgc tagctccggc gcaacaaact tctctctgct
     5821 gaaacaagcc ggagatgtcg aagagaatcc tggaccgacc gagtacaagc ccacggtgcg
     5881 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5941 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6001 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6061 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6121 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6181 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6241 agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6301 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6361 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6421 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt taagtcgaca atcaacctct
     6481 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6541 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6601 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6661 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6721 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6781 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6841 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6901 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6961 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7021 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     7081 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7141 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     7201 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7261 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7321 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     7381 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7441 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7501 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7561 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     7621 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     7681 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7741 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7801 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7861 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7921 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7981 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8041 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     8101 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8161 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8221 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8281 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8341 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     8401 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     8461 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     8521 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     8581 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     8641 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     8701 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     8761 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     8821 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     8881 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     8941 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     9001 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     9061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9181 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9241 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9301 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9361 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9421 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9481 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9541 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9601 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9661 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9721 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9781 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9841 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9901 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9961 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10021 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10081 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10141 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10201 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10261 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10321 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10381 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10441 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10501 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10561 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10621 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10681 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10741 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10801 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10861 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10921 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10981 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11041 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11101 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11161 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11221 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11281 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11341 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    11401 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    11461 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    11521 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
    11581 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
    11641 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    11701 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    11761 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    11821 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    11881 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    11941 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgg
//
