LOCUS       sequence-373499-       11309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged dCas12j2 KRAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: dCas12j2 KRAB
FEATURES             Location/Qualifiers
     source          1..11309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4715..4762
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4763..4786
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4820..5014
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             5081..5101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5114..5170
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5171..5767
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5664..5684
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5783..6371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5836..5856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6254..6265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6443..6676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6702..6719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6708..6932
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6978..7406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7065..7084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7275..7296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7415..7435)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7420..7749
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7600..7735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7662..7681
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7797..7844
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7863..8237
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8367..8488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8404..8423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8458..8477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8537..8553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8537..8553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8550..8572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8561..8577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8585..8591,8592..8609,8610..8615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8630..8651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8768..8785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8939..9527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9019..9038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9698..10489,10490..10558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10321..10340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10559..10663)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10738..10757)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10929..11308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatgccaa agccagccgt
     2461 ggagtctgag ttttctaagg tactcaagaa gcactttccg ggcgagcgat ttaggtctag
     2521 ctacatgaag cggggtggta aaatcttggc agcccagggt gaagaagcgg tcgtcgcgta
     2581 tctgcaaggc aagtccgagg aggaaccccc gaattttcag ccgccggcga aatgtcatgt
     2641 tgttacgaaa tcacgagatt tcgccgagtg gccaattatg aaggcctccg aagcaatcca
     2701 aaggtatatc tatgcgctct ctacgacgga acgggcagct tgcaagcctg gcaaatcttc
     2761 agagtcccac gcggcctggt tcgcggcaac tggcgtgtca aaccacggtt atagccatgt
     2821 tcaaggcctc aatcttatct tcgaccacac gctgggaaga tacgatggtg ttctgaaaaa
     2881 ggtgcagctg agaaatgaga aagcccgcgc ccggctggaa agtatcaacg cctctcgagc
     2941 cgacgaagga cttccagaaa taaaggcaga ggaggaagag gtcgctacaa atgaaaccgg
     3001 acaccttttg cagcctccgg ggatcaaccc aagtttctac gtttaccaga ctatttctcc
     3061 gcaggcttac aggccgcgag atgagattgt actgccgccc gagtatgccg gctacgtccg
     3121 agatccgaac gcccctatcc cccttggcgt ggttcggaat cggtgcgata ttcagaaggg
     3181 atgccctgga tacatccccg aatggcaaag agaggcaggt actgcaattt cccctaagac
     3241 gggtaaagcc gtcaccgttc ccggcctcag tccaaaaaaa aataaacgaa tgcgacgata
     3301 ctggaggtcc gagaaagaga aggcccaaga tgcactgctc gttactgtga gaatcggcac
     3361 tgactgggtc gtaatcgacg ttcgaggttt gctgcggaat gcgcggtggc gcaccattgc
     3421 gcccaaggat atatccttga atgccctctt ggatctcttt acaggcgacc cggtcataga
     3481 tgttcggaga aacattgtga ctttcaccta cactctggac gcttgcggta catatgctcg
     3541 caaatggact ctcaaaggga aacagactaa ggcaaccctc gataagttga ccgcaaccca
     3601 gaccgtggcc ctggtagcaa tagcccttgg acaaaccaat cccataagtg cgggtatcag
     3661 tagggtcacg caagaaaacg gggcacttca atgtgaacct ctggatcggt tcactctccc
     3721 tgatgatctg ctcaaggata tctccgcgta ccgaatcgct tgggatcgca acgaggagga
     3781 actgagggct aggtccgtcg aagcgctccc agaagctcaa caagctgaag tgagggctct
     3841 ggacggcgtt tctaaagaaa ccgccaggac ccagctctgc gcggacttcg gccttgatcc
     3901 caaacggctg ccttgggata aaatgagcag caacaccact ttcatcagtg aagcgttgct
     3961 tagtaattct gtgtctagag atcaggtttt ttttactcct gcgcctaaaa agggagcaaa
     4021 gaaaaaagcc cccgttgaag ttatgcggaa ggataggacc tgggcgaggg cctataaacc
     4081 acggctcagt gtggaagccc aaaagctgaa aaatgaggcc ttgtgggctc tcaagcgcac
     4141 ttctccagaa tacctcaagc tgagtcggag aaaagaggag ctttgtaggc gaagtattaa
     4201 ctacgtcatt gaaaaaacaa gacggaggac acaatgtcag atcgtgatac ctgtcataga
     4261 ggacttgaat gtgcgattct ttcacggttc agggaagcgc ctgcctggct gggataattt
     4321 tttcactgcg aagaaggaga acaggtggtt tatacagggc ctccacaaag cattcagcga
     4381 cttgcgaact catcgctcct tctacgtatt cgaagtccgc ccggagcgga cttcaataac
     4441 gtgcccaaaa tgcgggcact gcgaggttgg gaaccgggat ggggaggctt ttcagtgcct
     4501 tagttgcggc aaaacgtgca atgccgacct tgacgtggct acccataatc tgactcaagt
     4561 cgcccttaca ggaaaaacaa tgccgaaacg cgaggaacct agagatgccc agggcacagc
     4621 tccagcccga aaaacaaaga aggcgtcaaa gagcaaggct ccgccagccg aacgagagga
     4681 ccaaactcca gcacaggaac cgtcccagac ttccaagcga cctgccgcca caaagaaggc
     4741 tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat ccgatgctaa
     4801 gtcactgact gcctggtccc ggacactggt gaccttcaag gatgtgtttg tggacttcac
     4861 cagggaggag tggaagctgc tggacactgc tcagcagatc ctgtacagaa atgtgatgct
     4921 ggagaactat aagaacctgg tttccttggg ttatcagctt actaagccag atgtgatcct
     4981 ccggttggag aagggagaag agccctggct ggtggagaga gaaattcacc aagagaccca
     5041 tcctgattca gagactgcat ttgaaatcaa atcatcagtt ccgaaaaaga aacgcaaagt
     5101 tgctagctcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     5161 tcctggaccg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5221 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5281 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5341 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5401 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5461 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5521 caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg
     5581 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5641 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5701 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5761 tgcctgaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     5821 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5881 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5941 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6001 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6061 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6121 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6181 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6241 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6301 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6361 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6421 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6481 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6661 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6721 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6781 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6841 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6901 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6961 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7021 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7081 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7141 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7201 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7261 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7321 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7381 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7441 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7501 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7561 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7621 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7681 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7741 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     7801 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     7861 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     7921 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     7981 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     8041 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     8101 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     8161 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     8221 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     8281 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8341 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     8401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8461 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     8521 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8701 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9361 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9481 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9541 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9601 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9661 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9721 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9781 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9841 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9901 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9961 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10021 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10081 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10141 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10201 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10261 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10321 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10381 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10441 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10501 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10561 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10621 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10681 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
    10741 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    10801 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    10861 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    10921 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
    10981 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
    11041 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
    11101 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
    11161 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
    11221 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
    11281 ctacgtatta gtcatcgcta ttaccatgg
//
