LOCUS       sequence-373518-        4710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC44A5_STOP
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2267..2366)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2383..2402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2384..2402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2407..2423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2407..2423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2525..2544)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2536..3345
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2603..2622)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3492..4080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3981..4000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4234..4251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4410..4437)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4529..4615)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4664..4686
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4678..4695
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4679..4695
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa tgataccgag
      121 aagcctgccg acacaccatc cgaggaggag gattttggcg accccagaac ctacgatcct
      181 gacttcaagg gcccagtggc caacaggtct tgcacagatg tgctgtgctg tatgatcttt
      241 ctgctgtgca tcatcggcta catcgtgctg ggactggtgg catgggtgca cggcgatcca
      301 aggagagccg cctatcccac agacagccag ggacacttct gcggacagaa gggaacccct
      361 aacgagaata agacaatcct gttctacttc aacctgctgc gctgcacctc tcctagcgtg
      421 ctgctgaatc tgcagtgtcc aaccacacag atctgcgtga gcaagtgtcc agagaagttt
      481 ctgacctacg tggagatgca gctgctgtat acaaaggata agtcctactg ggaggactat
      541 cggcagttct gtaagaccac agccaagcca gtgaagtctc tgacccagct gctgctggac
      601 gatgactgcc ccacagccat cttccccagc aagccttttc tgcagcggtg cttccctgat
      661 ttttccacca agaacggcac cctgacaatc ggctctaaga tgatgttcca ggacggcaat
      721 ggaggaacac ggtccgtggt ggagctggga atcgcagcaa acggcatcaa taagctgctg
      781 gatgccaagt ctctgggcct gaaggtgttc gaggactacg ccagaacctg gtattggatc
      841 ctgatcggcc tgacaatcgc catggtgctg tcttggatct tcctgatcct gctgaggttt
      901 atcgccggct gcctgttctg ggtgtttatg atcggcgtga tcggcatcat cggctacggc
      961 atctggcact gttaccagca gtataccaac ctgcaggaga gacccagctc cgtgctgacc
     1021 atctacgata tcggcatcca gacaaatatc tccatgtatt ttgagctgca gcagacctgg
     1081 ttcaccttca tgatcatcct gtgcatcatc gaagtgatcg tgatcctgat gctgatcttc
     1141 ctgaggaatc gcatccgggt ggccatcatc ctgctgaagg agggcagcaa ggccatcggc
     1201 tacgtgcctt ccaccctggt gtatcccgcc ctgaccttca tcctgctgag catctgcatc
     1261 tgttactggg tggtgaccgc cgtgttcctg gccacatccg gagtgccagt gtataaagtg
     1321 atcgcaccag gaggacactg catccacgag aaccagacct gtgaccccga gatctttaat
     1381 accacagaga tcgcaaaggc atgccctggc gccctgtgca acttcgcctt ttacggcggc
     1441 aagagcctgt accaccagta tatccccacc ttccacgtgt ataacctgtt cgtgtttctg
     1501 tggctgatca attttgtgat cgccctggga cagtgcgcac tggcaggagc cttcgccacc
     1561 tactattggg ccatgaagaa gcctgatgac atcccaaggt accccctgtt caccgccttt
     1621 ggcagagcca tcaggtatca cacaggctcc ctggcctttg gctctctgat catcgccctg
     1681 atccagatgt tcaagatcgt gctggagtac ctggaccacc gcctgaagcg gacccagaac
     1741 acactgagca agttcctgca gtgctgtctg cgctgctgtt tttggtgcct ggagaatgcc
     1801 atcaagttcc tgaaccggaa tgcctacatc atgatcgcca tctatggcag aaacttctgt
     1861 aggtccgcca aggatgcctt taacctgctg atgagaaatg tgctgaaggt ggccgtgacc
     1921 gacgaggtga catacttcgt gctgtttctg ggcaagctgc tggtggcagg atctatcggc
     1981 gtgctggcct ttctgttctt tacccagagg ctgcccgtga tcgcacaggg acctgccagc
     2041 ctgaactact attgggtgcc actgctgaca gtgatcttcg gctcttacct gatcgcccac
     2101 ggcttcttta gcgtgtatgc catgtgcgtg gagaccatct tcatctgttt tctggaggat
     2161 ctggagagga acgacggatc caccgcaagg ccatacttcg tgacacctaa tctgcacggc
     2221 atcctgatca agaagcagct ggtgccccag aagcagaagg agtaagaccc agctttcttg
     2281 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     2341 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     2401 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2461 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2521 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2581 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2641 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2701 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2761 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2821 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2881 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2941 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     3001 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     3061 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     3121 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     3181 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     3241 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3301 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3361 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3421 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3481 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3541 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3601 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3661 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3721 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3781 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3841 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3901 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3961 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4021 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4081 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4141 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4201 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4261 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4321 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     4381 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4441 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4501 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4561 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4621 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4681 aaaacgacgg ccagtcttaa gctcgggccc
//
