LOCUS       sequence-37356-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Oct4 targeting donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, 
            Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, 
            Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Genetic engineering of human pluripotent cells using TALE nucleases.
  JOURNAL   Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
  PUBMED    21738127
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927."
COMMENT     Sequence Label: OCT4-eGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(816..832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(816..832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(829..851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    840..856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(864..870,871..888,889..894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cccggtangc tctcgccctt gctcaccatg ctagcgtttg aatgcatggg agagcccaga
       61 gtggtgacgg agacaggggg aaaggcttcc ccctcaggga aagggaccga ggagtacagt
      121 gcagtgaagt gagggctccc atagcctggg gtaccaaaat ggggccctgg ggccagagga
      181 aaggacactg gtccccctga gaaaggagac ccagcagcct caaaatcctc tcgttgtgca
      241 tagtcgctgc ttgatcgctt gcccttctgg cgccggttac agaaccacac tcggaccacc
      301 tgcaagtgaa tgacagaaag gagaatgaca ttagacaatg agctgagacg ggcctgactc
      361 tgcttggaca ttctatccaa agccaacagc cctagagcag ttagaggagg acattagaga
      421 atgagctgag acaggcctga ctgcttggac attctgtcca aagccaacag ccctagagca
      481 gttggaggag ccagagctag ggaaagcgag gtggtgacag gggaaagaga tggagcccgc
      541 agagagacat ggcactcaca tccttctcga gcccaagctg ctgggcgatg tggctgatct
      601 gctgcagtgt gggtttcggg cactgcagga acaaattctc caggttgcct ctcactcggt
      661 tctcgatact ggttcgcttt ctctttcggg cctgcacgag ggtttctgct ttgcatatct
      721 gtgcaggtgg gacctgcagg aaaagggcga attccagcac actggcggcc gttactagtg
      781 gatccgagct cggtaccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      901 ggtgcctatg a
//
