LOCUS       sequence-37358-e        1617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Kumar D, Shadrach JL, Wagers AJ, Lassar AB
  TITLE     Id3 is a direct transcriptional target of Pax7 in quiescent 
            satellite cells.
  JOURNAL   Mol Biol Cell. 2009 Jul;20(14):3170-7. Epub 2009 May 20.
  PUBMED    19458195
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2009-07"; volume: "20(14)"; pages: "3170-7. Epub 2009
            May 20"
COMMENT     Sequence Label: pOZ-Pax7-FH-C-puro
FEATURES             Location/Qualifiers
     source          1..1617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1549..1572
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1588..1614
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1588..1614
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agatctgcca ccatggcggc gctgcccggc acggtaccga gaatgatgag acccggcccg
       61 gggcagaact acccgcgcac cggcttcccc ctggaggtgt ccacccctct tggccaaggc
      121 cgggtcaatc agcttggtgg ggtcttcatc aatggtcgac ccctgccgaa ccacatccgt
      181 cacaagatag tggaaatggc ccaccatggc atccggccct gcgtcatctc ccgtcagctc
      241 cgtgtttctc atggttgtgt ctccaagatt ctgtgccgat atcaggagac tgggtccatc
      301 cggcccgggg ctatcggagg cagcaagccc agacaggtgg cgactccgga tgtggagaaa
      361 aagattgagg agtataagag agagaacccc gggatgttca gctgggaaat ccgggaccgg
      421 ctgctgaagg acggtcactg cgaccgaagc acggtgccct cagtgagttc gattagccga
      481 gtgctcagaa tcaagttcgg gaagaaagag gacgacgagg aaggagacaa gaaagaagaa
      541 gatggcgaga agaaagccaa acacagcatc gatggcatcc tgggcgacaa agggaaccgt
      601 ctggatgagg gctcagatgt ggaatcagaa cccgacctcc ccctgaagcg caagcagcgc
      661 cgcagtcgga ccacgttcac agccgagcag ctggaggagc tagagaaggc ctttgagagg
      721 acccactacc cggacatcta cacccgggag gagctggcac agaggaccaa gctcacggag
      781 gcacgcgtcc aggtctggtt cagtaaccgg cgtgcccgct ggcgcaagca ggcaggagct
      841 aaccagctgg ccgccttcaa ccaccttctg ccgggaggtt tcccacccac cggcatgccc
      901 acgctgccac cctaccagct gccggactct acctacccca ccaccaccat ctcccaagat
      961 gggggcagca cagtacacag gccccagccc cttccgccat caaccatgca tcagggtggg
     1021 ctggctgcgg ccgctgcagc agcagacacc agctctgcct acggagcccg ccacagcttc
     1081 tccagctact ctgacagctt catgaaccct ggggctccct ccaaccacat gaaccctgtc
     1141 agcaatggcc tgtctcctca ggtcatgagc atccttagca acccgagtgc cgtgcctcca
     1201 cagccccagg ccgacttctc catctccccg ctgcatggag gcctggactc ggcttcctcc
     1261 atctcagcca gctgcagcca acgggccgac tccatcaagc caggagacag cttgcccacg
     1321 tcccagtctt actgcccacc caccttcagc accactggct acagtgtgga ccctgtggct
     1381 ggctaccagt acagccagta tggccaaact gctgttgatt acctggccaa aaacgtgagc
     1441 ctgtccacac agcgccgtat gaagcttggg gaacactccg ctgtgctggg acttcttcct
     1501 gtggaaacgg gacaagccta cctcgaggaa tccgcggccg ctggaggaga ctacaaggac
     1561 gacgatgaca agtcggccgc tggaggatac ccctacgacg tgcccgacta cgcctag
//
