LOCUS       sequence-373597-       11654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged PlmCas12e.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: PlmCas12e
FEATURES             Location/Qualifiers
     source          1..11654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5378..5425
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5426..5449
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5459..5515
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5516..6112
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6009..6029
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6128..6716
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6181..6201)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6599..6610)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6788..7021
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7047..7064)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7053..7277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7323..7751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7410..7429)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7620..7641
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7760..7780)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7765..8094
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7945..8080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8007..8026
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8142..8189
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8208..8582
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8712..8833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8749..8768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8803..8822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8882..8898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8882..8898)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8895..8917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8906..8922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8930..8936,8937..8954,8955..8960))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8975..8996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9113..9130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9284..9872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9364..9383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10043..10834,10835..10903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10666..10685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10904..11008)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11083..11102)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11274..11653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatgcaag agatcaagag
     2461 aatcaacaag atcagaagga gactggtcaa ggacagcaac acaaagaagg ccggcaagac
     2521 aggccccatg aaaaccctgc tcgtcagagt gatgacccct gacctgagag agcggctgga
     2581 aaacctgaga aagaagcccg agaacatccc tcagcctatc agcaacacca gcagggccaa
     2641 cctgaacaag ctgctgaccg actacaccga gatgaagaaa gccatcctgc acgtgtactg
     2701 ggaagagttc cagaaagacc ccgtgggcct gatgagcaga gttgctcagc ccgctcctaa
     2761 gaacatcgac cagagaaagc tgatccccgt gaaggacggc aacgagagac tgacctctag
     2821 cggctttgcc tgcagccagt gttgccagcc tctgtacgtg tacaagctgg aacaagtgaa
     2881 cgacaagggc aagccccaca ccaactactt cggcagatgc aacgtgtccg agcacgagag
     2941 gctgatcctg ctgtctcctc acaagcccga ggccaacgat gagctggtca catacagcct
     3001 gggcaagttc ggacagagag ccctggactt ctacagcatc cacgtgacca gggagagcaa
     3061 tcaccctgtg aagcccctgg aacagatcgg cggcaatagc tgtgcctctg gacctgtggg
     3121 aaaagccctg agcgacgcct gtatgggagc cgtggcatcc ttcctgacca agtaccagga
     3181 catcatcctg gaacaccaga aagtgatcaa gaagaacgag aaaagactgg ccaacctcaa
     3241 ggatatcgcc agcgctaacg gcctggcctt tcctaagatc accctgcctc cacagcctca
     3301 caccaaagag ggcatcgagg cctacaacaa cgtggtggcc cagatcgtga tttgggtcaa
     3361 cctgaatctg tggcagaagc tgaagatcgg cagggacgaa gccaagccac tgcagagact
     3421 gaagggcttc cctagcttcc ctctggtgga aagacaggcc aatgaagtgg attggtggga
     3481 catggtctgc aacgtgaaga agctgatcaa cgagaagaaa gaggatggca aggttttctg
     3541 gcagaacctg gccggctaca agagacaaga agccctgctg ccttacctga gcagcgaaga
     3601 ggaccggaag aagggcaaga agttcgccag ataccagttc ggcgacctgc tgctgcacct
     3661 ggaaaagaag cacggcgagg actggggcaa agtgtacgat gaggcctggg agagaatcga
     3721 caagaaggtg gaaggcctga gcaagcacat taagctggaa gaggaaagaa ggagcgagga
     3781 cgcccaatct aaagccgctc tgaccgattg gctgagagcc aaggccagct ttgtgatcga
     3841 gggcctgaaa gaggccgaca aggacgagtt ctgcagatgc gagctgaagc tgcagaagtg
     3901 gtacggcgat ctgagaggca agcccttcgc cattgaggcc gagaacagca tcctggacat
     3961 cagcggcttc agcaagcagt acaactgcgc cttcatttgg cagaaagacg gcgtcaagaa
     4021 actgaacctg tacctgatca tcaattactt caaaggcggc aagctgcggt tcaagaagat
     4081 caaacccgag gccttcgagg ctaacagatt ctacaccgtg atcaacaaaa agtccggcga
     4141 gatcgtgccc atggaagtga acttcaactt cgacgacccc aacctgatta tcctgcctct
     4201 ggccttcggc aagagacagg gcagagagtt catctggaac gatctgctga gcctggaaac
     4261 cggctctctg aagctggcca atggcagagt gatcgagaaa accctgtaca acaggagaac
     4321 cagacaggac gagcctgctc tgtttgtggc cctgaccttc gagagaagag aggtgctgga
     4381 cagcagcaac atcaagccca tgaacctgat cggcatcgac cggggcgaga atatccctgc
     4441 tgtgatcgcc ctgacagacc ctgaaggatg cccactgagc agattcaagg actccctggg
     4501 caaccctaca cacatcctga gaatcggcga gagctacaaa gagaagcaga ggacaatcca
     4561 ggccgccaaa gaggtggaac agagaagagc cggcggatac tctaggaagt acgccagcaa
     4621 ggccaagaat ctggccgacg acatggtccg aaacaccgcc agagatctgc tgtactacgc
     4681 cgtgacacag gacgccatgc tgatcttcga gaatctgagc agaggcttcg gccggcaggg
     4741 caagagaacc tttatggccg agaggcagta caccagaatg gaagattggc tcacagctaa
     4801 actggcctac gagggactgc ccagcaagac ctacctgtcc aaaacactgg cccagtatac
     4861 ctccaagacc tgcagcaatt gcggcttcac catcaccagc gccgactacg acagagtgct
     4921 ggaaaagctc aagaaaaccg ccaccggctg gatgaccacc atcaacggca aagagctgaa
     4981 ggttgagggc cagatcacct actacaacag gtacaagagg cagaacgtcg tgaaggatct
     5041 gagcgtggaa ctggacagac tgagcgaaga gagcgtgaac aacgacatca gcagctggac
     5101 aaagggcaga tcaggcgagg ctctgagcct gctgaagaag aggtttagcc acagacctgt
     5161 gcaagagaag ttcgtgtgcc tgaactgcgg cttcgagaca cacgccgatg aacaggctgc
     5221 cctgaacatt gccagaagct ggctgttcct gagaagccaa gagtacaaga agtaccagac
     5281 caacaagacc accggcaaca ccgacaagag ggcctttgtg gaaacctggc agagcttcta
     5341 cagaaaaaag ctgaaagaag tctggaagcc cgccgtgaag cgacctgccg ccacaaagaa
     5401 ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg gatccggcgc
     5461 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgaccga
     5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5821 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5881 gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta
     6121 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6181 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6241 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6301 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6361 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6421 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6481 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6541 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6601 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6661 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     6721 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6781 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6841 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6901 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6961 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7021 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     7081 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7141 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7201 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     7261 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     7321 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7381 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7501 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7561 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7621 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7681 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7741 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7801 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7861 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7921 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7981 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     8041 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     8101 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     8161 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     8221 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     8281 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     8341 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     8401 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     8461 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     8521 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     8581 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8641 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8701 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8821 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     8881 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8941 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9001 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9061 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10021 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10081 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10141 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10201 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10261 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10321 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10381 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10441 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10501 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10561 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10621 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10681 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10741 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10801 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10861 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    11041 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    11101 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    11161 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    11221 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
    11281 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    11341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    11401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    11461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    11521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    11581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
    11641 cgctattacc atgg
//
