LOCUS       sequence-37361-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, 
            Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, 
            Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Genetic engineering of human pluripotent cells using TALE nucleases.
  JOURNAL   Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
  PUBMED    21738127
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927."
COMMENT     Sequence Label: OCT4-eGFP-2A-Puro
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(808..824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(808..824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(821..843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    832..848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(856..862,863..880,881..886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gctctcgccc ttgctcacca tgctagcgtt tgaatgcatg ggagagccca gagtggtgac
       61 ggagacaggg ggaaaggctt ccccctcagg gaaagggacc gaggagtaca gtgcagtgaa
      121 gtgagggctc ccatagcctg gggtaccaaa atggggccct ggggccagag gaaaggacac
      181 tggtccccct gagaaaggag acccagcagc ctcaaaatcc tctcgttgtg catagtcgct
      241 gcttgatcgc ttgcccttct ggcgccggtt acagaaccac actcggacca cctgcaagtg
      301 aatgacagaa aggagaatga cattagacaa tgagctgaga cgggcctgac tctgcttgga
      361 cattctatcc aaagccaaca gccctagagc agttagagga ggacattaga gaatgagctg
      421 agacaggcct gactgcttgg acattctgtc caaagccaac agccctagag cagttggagg
      481 agccagagct agggaaagcg aggtggtgac aggggaaaga gatggagccc gcagagagac
      541 atggcactca catccttctc gagcccaagc tgctgggcga tgtggctgat ctgctgcagt
      601 gtgggtttcg ggcactgcag gaacaaattc tccaggttgc ctctcactcg gttctcgata
      661 ctggttcgct ttctctttcg ggcctgcacg agggtttctg ctttgcatat ctgtgcaggt
      721 gggacctgca ggaaaagggc gaattccagc acactggcgg ccgttactag tggatccgag
      781 ctcggtacca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      841 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagctg
//
