LOCUS       sequence-373667-       10613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PSKH2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10613)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PSKH2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..10613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..76
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             145..744
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(145..164)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     641..661
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             882..1115
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1187..1308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1224..1243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1278..1297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1348..1483
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1503..1522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1504..1522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1532..1549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1532..1548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1541..1563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1690..2145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1777..1796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1987..2008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2171..2275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2276..2344,2345..3136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3307..3895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3796..3815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4049..4066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4183..4204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4219..4224,4225..4242,4243..4249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4257..4273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4262..4284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4281..4297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4281..4297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4316..4336
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4318..4336
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4364..4590
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4591..4771
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4818..4943
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5436..5669
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5854..5898
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6165..6281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6313..6333
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6333..7544
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7381..7400
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    7545..7569
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7572..7581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8737..8761)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8763..8804
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8811..9581
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(9587..9607)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    9636..10224
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9689..9709)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10107..10118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10238..10613,1..124)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10273..10296)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
       61 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
      121 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
      181 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
      241 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
      301 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
      361 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
      421 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
      481 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
      541 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
      601 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
      661 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
      721 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
      781 agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc
      841 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc
      901 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
      961 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1021 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1081 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     1141 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     1201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     1321 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     1381 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     1441 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     1501 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     1561 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1621 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1681 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1741 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1801 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     1861 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     1921 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     1981 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2041 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2101 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     2161 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2221 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2281 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2341 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2401 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2461 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2521 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2581 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2641 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2701 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2761 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2821 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2881 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2941 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3001 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3061 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3121 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3181 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3241 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3361 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3421 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3481 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3541 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3601 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3661 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3721 cgaagggaga aaggtggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3781 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3841 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3901 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3961 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4021 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4081 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     4141 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     4201 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     4261 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     4321 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     4381 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     4441 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     4501 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     4561 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     4621 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4681 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4741 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     4801 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     4861 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     4921 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     4981 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     5041 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     5101 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     5161 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     5221 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     5281 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     5341 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     5401 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     5461 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     5521 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     5581 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     5641 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     5701 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     5761 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     5821 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     5881 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     5941 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     6001 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     6061 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     6121 agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg
     6181 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     6241 actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag tgtggtggaa
     6301 ttctgcagat atcaactttg tatagaaaag ttggcctccg cgccgggttt tggcgcctcc
     6361 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
     6421 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
     6481 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
     6541 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
     6601 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
     6661 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
     6721 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
     6781 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
     6841 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
     6901 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
     6961 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
     7021 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
     7081 ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc
     7141 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     7201 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
     7261 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     7321 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     7381 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     7441 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     7501 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacacaagt ttgtacaaaa
     7561 aagcaggctt agccaccatg gggtgcggcg ccagcaggaa ggtggtcccg gggccaccag
     7621 cgctggcttg ggccaagcac gaaggtcaaa accaagccgg cgtcggaggc gcggggcctg
     7681 ggcccgaggc ggcggcccag gcggcgcaga ggatacaggt ggctcgcttc cgagccaagt
     7741 tcgacccccg ggtccttgcc agatatgaca tcaaagctct tattgggaca ggcagtttca
     7801 gcagggttgt cagggtagag cagaagacca ccaagaaacc ttttgcaata aaagtgatgg
     7861 aaaccagaga gagggaaggt agagaagcgt gcgtgtctga gctgagcgtc ctgcggcggg
     7921 ttagccatcg ttacattgtc cagctcatgg agatctttga gactgaggat caagtttaca
     7981 tggtaatgga gctggctacc ggaggggagc tctttgatcg actcattgct cagggatcct
     8041 ttacagagcg ggatgccgtc aggatcctcc agatggttgc tgatgggatt aggtatttgc
     8101 atgcgctgca gataactcat aggaatctaa agcctgaaaa cctcttatac tatcatccag
     8161 gtgaagagtc gaaaatttta attacagatt ttggtttggc atactccggg aaaaaaagtg
     8221 gtgactggac aatgaagaca ctctgtggga ccccagagta catagctcct gaggttttgc
     8281 taaggaagcc ttataccagt gcagtggaca tgtgggctct tggtgtgatc acatatgctt
     8341 tacttagcgg attcctgcct tttgatgatg aaagccagac aaggctttac aggaagattc
     8401 tgaaaggcaa atataattat acaggagagc cttggccaag catttcccac ttggcgaagg
     8461 actttataga caaactactg attttggagg ctggtcatcg catgtcagct ggccaggccc
     8521 tggaccatcc ctgggtgatc accatggctg cagggtcttc catgaagaat ctccagaggg
     8581 ccatatcccg aaacctcatg cagagggcct ctccccactc tcagagtcct ggatctgcac
     8641 agtcttctaa gtcacattat tctcacaaat ccaggcatat gtggagcaag agaaacttaa
     8701 ggatagtaga atcgccactg tctgcgcttt tgtcaaaccc agctttcttg tacaaagtgg
     8761 gcggcaagcc catccccaac cccctgctgg gcctggacag caccgctagc atcccgctgc
     8821 tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg
     8881 tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt
     8941 gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt
     9001 ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg
     9061 gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca
     9121 aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc
     9181 tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta
     9241 acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg
     9301 aagcagggat taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg
     9361 cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc
     9421 tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga
     9481 ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac
     9541 cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gtaagccaac tttattatac
     9601 atagttgata tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt
     9661 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     9721 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     9781 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     9841 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     9901 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     9961 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
    10021 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
    10081 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
    10141 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
    10201 tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc gcttttccca
    10261 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
    10321 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
    10381 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
    10441 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
    10501 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
    10561 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgg
//
