LOCUS       sequence-373669-       10991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged Cas12j2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: Cas12j2
FEATURES             Location/Qualifiers
     source          1..10991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4715..4762
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4763..4786
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4796..4852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4853..5449
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5346..5366
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5465..6053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5518..5538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5936..5947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6125..6358
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6384..6401)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6390..6614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6660..7088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6747..6766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6957..6978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7097..7117)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7102..7431
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7282..7417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7344..7363
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7479..7526
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7545..7919
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8049..8170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8086..8105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8140..8159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8219..8235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8219..8235)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8232..8254)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8243..8259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8267..8273,8274..8291,8292..8297))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8312..8333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8450..8467)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8621..9209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8701..8720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9380..10171,10172..10240))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10003..10022
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10241..10345)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10420..10439)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10611..10990
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatgccaa agccagccgt
     2461 ggagtctgag ttttctaagg tactcaagaa gcactttccg ggcgagcgat ttaggtctag
     2521 ctacatgaag cggggtggta aaatcttggc agcccagggt gaagaagcgg tcgtcgcgta
     2581 tctgcaaggc aagtccgagg aggaaccccc gaattttcag ccgccggcga aatgtcatgt
     2641 tgttacgaaa tcacgagatt tcgccgagtg gccaattatg aaggcctccg aagcaatcca
     2701 aaggtatatc tatgcgctct ctacgacgga acgggcagct tgcaagcctg gcaaatcttc
     2761 agagtcccac gcggcctggt tcgcggcaac tggcgtgtca aaccacggtt atagccatgt
     2821 tcaaggcctc aatcttatct tcgaccacac gctgggaaga tacgatggtg ttctgaaaaa
     2881 ggtgcagctg agaaatgaga aagcccgcgc ccggctggaa agtatcaacg cctctcgagc
     2941 cgacgaagga cttccagaaa taaaggcaga ggaggaagag gtcgctacaa atgaaaccgg
     3001 acaccttttg cagcctccgg ggatcaaccc aagtttctac gtttaccaga ctatttctcc
     3061 gcaggcttac aggccgcgag atgagattgt actgccgccc gagtatgccg gctacgtccg
     3121 agatccgaac gcccctatcc cccttggcgt ggttcggaat cggtgcgata ttcagaaggg
     3181 atgccctgga tacatccccg aatggcaaag agaggcaggt actgcaattt cccctaagac
     3241 gggtaaagcc gtcaccgttc ccggcctcag tccaaaaaaa aataaacgaa tgcgacgata
     3301 ctggaggtcc gagaaagaga aggcccaaga tgcactgctc gttactgtga gaatcggcac
     3361 tgactgggtc gtaatcgacg ttcgaggttt gctgcggaat gcgcggtggc gcaccattgc
     3421 gcccaaggat atatccttga atgccctctt ggatctcttt acaggcgacc cggtcataga
     3481 tgttcggaga aacattgtga ctttcaccta cactctggac gcttgcggta catatgctcg
     3541 caaatggact ctcaaaggga aacagactaa ggcaaccctc gataagttga ccgcaaccca
     3601 gaccgtggcc ctggtagcaa tagaccttgg acaaaccaat cccataagtg cgggtatcag
     3661 tagggtcacg caagaaaacg gggcacttca atgtgaacct ctggatcggt tcactctccc
     3721 tgatgatctg ctcaaggata tctccgcgta ccgaatcgct tgggatcgca acgaggagga
     3781 actgagggct aggtccgtcg aagcgctccc agaagctcaa caagctgaag tgagggctct
     3841 ggacggcgtt tctaaagaaa ccgccaggac ccagctctgc gcggacttcg gccttgatcc
     3901 caaacggctg ccttgggata aaatgagcag caacaccact ttcatcagtg aagcgttgct
     3961 tagtaattct gtgtctagag atcaggtttt ttttactcct gcgcctaaaa agggagcaaa
     4021 gaaaaaagcc cccgttgaag ttatgcggaa ggataggacc tgggcgaggg cctataaacc
     4081 acggctcagt gtggaagccc aaaagctgaa aaatgaggcc ttgtgggctc tcaagcgcac
     4141 ttctccagaa tacctcaagc tgagtcggag aaaagaggag ctttgtaggc gaagtattaa
     4201 ctacgtcatt gaaaaaacaa gacggaggac acaatgtcag atcgtgatac ctgtcataga
     4261 ggacttgaat gtgcgattct ttcacggttc agggaagcgc ctgcctggct gggataattt
     4321 tttcactgcg aagaaggaga acaggtggtt tatacagggc ctccacaaag cattcagcga
     4381 cttgcgaact catcgctcct tctacgtatt cgaagtccgc ccggagcgga cttcaataac
     4441 gtgcccaaaa tgcgggcact gcgaggttgg gaaccgggat ggggaggctt ttcagtgcct
     4501 tagttgcggc aaaacgtgca atgccgacct tgacgtggct acccataatc tgactcaagt
     4561 cgcccttaca ggaaaaacaa tgccgaaacg cgaggaacct agagatgccc agggcacagc
     4621 tccagcccga aaaacaaaga aggcgtcaaa gagcaaggct ccgccagccg aacgagagga
     4681 ccaaactcca gcacaggaac cgtcccagac ttccaagcga cctgccgcca caaagaaggc
     4741 tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac
     4801 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgaccgagta
     4861 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4921 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4981 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5041 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5101 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5161 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5221 cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5281 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5341 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5401 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt
     5461 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5521 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5581 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5641 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5701 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5761 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5821 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5881 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5941 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6001 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     6061 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6121 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6181 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6241 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6301 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     6361 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6421 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6481 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6541 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     6601 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     6661 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6721 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6781 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6841 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6901 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6961 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7021 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7081 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7141 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7201 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7261 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7321 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     7381 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     7441 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     7501 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     7561 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     7621 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     7681 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     7741 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     7801 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     7861 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     7921 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7981 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8041 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8161 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8221 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8281 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8341 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8401 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8461 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8521 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8581 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8641 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8701 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8761 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8821 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8881 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8941 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9001 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9061 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9121 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9181 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9241 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9301 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9361 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9421 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9481 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9541 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9601 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9661 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9721 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9781 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9841 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9901 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9961 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10021 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10081 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10141 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10201 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10261 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10321 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    10381 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
    10441 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
    10501 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
    10561 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
    10621 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
    10681 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
    10741 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
    10801 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
    10861 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    10921 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    10981 tattaccatg g
//
