LOCUS       sequence-37367-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, 
            Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, 
            Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Genetic engineering of human pluripotent cells using TALE nucleases.
  JOURNAL   Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
  PUBMED    21738127
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927."
COMMENT     Sequence Label: PITX3-eGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(635..651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(635..651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(648..670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    659..675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(683..689,690..707,708..713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    728..749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(866..883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccggttang ctcctcgccc ttgctcacca tgctagctac gggcctttcc acggcgtact
       61 ggcacggact aaggttggct gccgggggcg gcccgtgcac agcggggtag ctgaaggagg
      121 cgtgctgttt ggctttgagc cgcaggctgg ccaggctcga gttacacggg tcccgataga
      181 cgtaggggga agaggcggca gccgcggcgg cggcggcggc cgaggcataa gggcaggaca
      241 cggccccgga ggacacggcg gccggagcca gcccgggggg gcccccgccc aggccctgca
      301 gggccccagg ccctggcacg gtgcccgggg cagccgcggc ggagggcacc atggaggcgg
      361 cgatggagct gggtggcgag aagacgggct gcgaagccag aggccccacg ttgaccgagt
      421 tgaaggcgaa tggaaaggtc ttggcggcga gcggcggggc aagagccttg ggcggccagt
      481 tgccgtacga gtagccgggg tacacctcct cgtagggcgg caccagcccc ccgagcggcg
      541 ccgcgaagct gcctttgcat agctcggcct gctggctgcg ctcgcgcttc cgccatttgg
      601 atccgagctc ggtaccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcntcgt cntcgtgcgc
      901 g
//
