LOCUS       sequence-373670-       10361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10361)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        108..212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(213..281,282..1073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1244..1832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1733..1752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1986..2003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2120..2141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2156..2161,2162..2179,2180..2186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2194..2210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2199..2221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2218..2234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2218..2234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2253..2273
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2255..2273
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2301..2527
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2528..2708
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2755..2880
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3373..3606
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3791..3835
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4102..4218
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4252..4272
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4272..5483
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5320..5339
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5487..5507)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5508..5517
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5520..6290
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6297..6338
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6339..6363
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7267..7291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7321..7909
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7374..7394)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7792..7803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7923..8422
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7958..7981)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8355..8374
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8443..9042
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8443..8462)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8939..8959
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9180..9413
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9485..9606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9522..9541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9576..9595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9646..9781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9801..9820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9802..9820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9830..9847)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9830..9846)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9839..9861)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(9988..10361,1..82)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10075..10094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10285..10306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
       61 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      421 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      721 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
      961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2041 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2101 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2161 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2221 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     2281 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     2341 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     2401 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     2461 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     2521 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2701 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2761 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2821 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2881 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     2941 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3001 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3061 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3121 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3181 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3241 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3301 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3361 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3421 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3481 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3541 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3601 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3661 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3721 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3781 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3841 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3901 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     3961 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4021 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4081 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
     4141 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     4201 tacaaaattc aaaattttat cgtccagtgt ggtggaattc tgcagatatc acaactttgt
     4261 atagaaaagt tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     4321 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     4381 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     4441 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     4501 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     4561 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     4621 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     4681 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     4741 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     4801 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     4861 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     4921 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     4981 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     5041 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     5101 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     5161 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     5221 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     5281 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     5341 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     5401 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     5461 cgtttttggc ttttttgtta gacggccaac ttttgtatac aaagtgggcc accatgggaa
     5521 tcccgctgct gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc
     5581 tgcctgtggt cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg
     5641 gggatgcttg cattgcagaa tatcagcagg cagggagagg aagcagaggg aggaaatggt
     5701 tctctccttt tggagctaac ctgtacctga gtatgttttg gcgcctgaag cggggaccag
     5761 cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg
     5821 gagcagacaa ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg
     5881 caggcatcct ggtggagctg gccggaataa caggcgatgc tgcacagatc gtcattggcg
     5941 ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca
     6001 cactgcagga agcagggatt aacctggaca ggaatactct ggccgctatg ctgatccgag
     6061 agctgcgggc agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgtcacggt
     6121 gggagaagct ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga
     6181 ttttcgggat tagccggggg attgataaac agggagccct gctgctggaa caggacggag
     6241 ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag gctagcggca
     6301 agcccatccc caaccccctg ctgggcctgg acagcaccac aagtttgtac aaaaaagcag
     6361 gctccatcat ggagaacttc caaaaggtgg aaaagatcgg agagggcacg tacggagttg
     6421 tgtacaaagc cagaaacaag ttgacgggag aggtggtggc gcttaagaaa atccgcctgg
     6481 acactgagac tgagggtgtg cccagtactg ccatccgaga gatctctctg cttaaggagc
     6541 ttaaccatcc taatattgtc aagctgctgg atgtcattca cacagaaaat aaactctacc
     6601 tggtttttga atttctgcac caagatctca agaaattcat ggatgcctct gctctcactg
     6661 gcattcctct tcccctcatc aagagctatc tgttccagct gctccagggc ctagctttct
     6721 gccattctca tcgggtcctc caccgagacc ttaaacctca gaatctgctt attaacacag
     6781 agggggccat caagctagca gactttggac tagccagagc ttttggagtc cctgttcgta
     6841 cttacaccca tgaggtggtg accctgtggt accgagctcc tgaaatcctc ctgggctgca
     6901 aatattattc cacagctgtg gacatctgga gcctgggctg catctttgct gagatggtga
     6961 ctcgccgggc cctattccct ggagattctg agattgacca gctcttccgg atctttcgga
     7021 ctctggggac cccagatgag gtggtgtggc caggagttac ttctatgcct gattacaagc
     7081 caagtttccc caagtgggcc cggcaagatt ttagtaaagt tgtacctccc ctggatgaag
     7141 atggacggag cttgttatcg caaatgctgc actacgaccc taacaagcgg atttcggcca
     7201 aggcagccct ggctcaccct ttcttccagg atgtgaccaa gccagtaccc catcttcgac
     7261 tctaggaccc agctttcttg tacaaagtgg tgatatccag cacagtggcg gccgctcgac
     7321 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7381 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7441 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7501 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7561 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7621 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7681 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7741 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7801 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7861 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
     7921 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     7981 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     8041 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     8101 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     8161 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     8221 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     8281 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     8341 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     8401 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     8461 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8521 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8581 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8641 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8701 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8761 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8821 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8881 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8941 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     9001 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     9061 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
     9121 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9181 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     9241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     9421 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9481 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9541 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9601 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     9661 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9721 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9781 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     9841 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     9901 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     9961 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    10021 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    10081 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10141 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10201 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10261 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10321 ctatctcggt ctattctttt gatttataag ggattttgcc g
//
