LOCUS       sequence-373672-       11678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged DpbCas12e.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 11678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: DpbCas12e
FEATURES             Location/Qualifiers
     source          1..11678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2438..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5402..5449
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5450..5473
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5483..5539
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5540..6136
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6033..6053
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6152..6740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6205..6225)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6623..6634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6812..7045
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7071..7088)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7077..7301
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7347..7775
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7434..7453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7644..7665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7784..7804)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7789..8118
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7969..8104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8031..8050
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8166..8213
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8232..8606
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8736..8857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8773..8792)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8827..8846
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8906..8922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8906..8922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8919..8941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8930..8946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8954..8960,8961..8978,8979..8984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8999..9020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9137..9154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9308..9896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9388..9407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10067..10858,10859..10927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10690..10709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10928..11032)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11107..11126)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11298..11677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaaa agagaatcaa
     2461 caagatcagg aagaagctga gcgccgacaa cgccaccaag cctgtgtcta ggagtggccc
     2521 catgaaaacc ctgctcgtca gagtgatgac cgacgacctg aagaagagac tggaaaagag
     2581 gaggaagaaa cccgaagtga tgccccaagt gatcagcaac aacgccgcca acaacctgag
     2641 aatgctgctg gacgactaca ccaagatgaa ggaagccatc ctgcaggtct actggcaaga
     2701 gttcaaggac gaccacgtgg gcctgatgtg caagtttgcc cagcctgcca gcaagaagat
     2761 cgaccagaac aagctgaagc ccgagatgga cgagaagggc aatctgacca cagccggctt
     2821 tgcctgctct cagtgtggcc agcctctgtt cgtgtacaag ctggaacagg tgtccgagaa
     2881 aggcaaggcc tacaccaact acttcggcag atgtaacgtg gccgagcacg agaagctgat
     2941 tctgctggcc cagctgaaac ctgagaagga ctctgatgag gccgtgacct acagcctggg
     3001 caagtttgga cagagagccc tggacttcta cagcatccac gtgaccaaag aaagcacaca
     3061 ccccgtgaag cccctggctc agatcgccgg caatagatac gcctctggac ctgtgggcaa
     3121 agccctgtcc gatgcctgca tgggaacaat cgccagcttc ctgagcaagt accaggacat
     3181 catcatcgag caccagaagg tggtcaaggg caaccagaag agactggaaa gcctgaggga
     3241 gctggccggc aaagagaacc tggaataccc cagcgtgacc ctgcctcctc agcctcacac
     3301 aaaagaaggc gtggacgcct acaacgaagt gatcgccaga gtgagaatgt gggtcaacct
     3361 gaacctgtgg cagaagctga aactgtccag ggacgacgcc aagcctctgc tgagactgaa
     3421 gggcttccct agcttccccg tggtggaaag aagggagaat gaggtggact ggtggaacac
     3481 catcaacgaa gtcaagaaac tgatcgatgc caagagagac atgggcagag tgttttggag
     3541 cggagtgacc gccgagaaga gaaacaccat cctggaaggc tacaactacc tgcctaacga
     3601 gaacgaccac aagaagagag agggcagcct ggaaaaccct aagaagcccg ccaagagaca
     3661 gttcggcgac ctgctgctgt acctggagaa gaagtatgcc ggcgactggg gcaaagtgtt
     3721 cgatgaggcc tgggagagaa tcgacaagaa gattgccggc ctgaccagcc acatcgagag
     3781 agaggaagcc agaaacgccg aggacgccca gtctaaagcc gtgctgacag attggctgag
     3841 agccaaggcc agcttcgtgc tggaaaggct gaaagaaatg gatgagaaag agttctacgc
     3901 ctgcgagatc cagctgcaga agtggtacgg ggacctgaga ggcaatccct ttgccgtgga
     3961 agccgagaac agagtggtgg acatcagcgg ctttagcatc ggctctgacg gccactccat
     4021 ccagtacagg aacctgctgg cctggaagta cctggaaaat ggcaagagag agttctacct
     4081 gctgatgaac tacggcaaga agggcagaat cagattcacc gacggcaccg acatcaagaa
     4141 aagcggcaag tggcagggcc tgctgtatgg cggcggaaag gccaaagtga tcgacctgac
     4201 cttcgatccc gacgacgagc agctgatcat tctgcctctg gccttcggca ccagacaggg
     4261 cagagagttc atctggaacg atctgctgtc cctggaaacc ggcctgatca agctggccaa
     4321 cggcagggtg atcgagaaaa ccatctacaa caagaaaatc ggcagagatg agcccgcact
     4381 gtttgtggcc ctgacctttg agaggagaga agtggtcgat cccagcaaca tcaagcccgt
     4441 gaacctgatc ggagtggaca gaggcgagaa tatccctgcc gtgattgccc tgacagaccc
     4501 tgagggatgt cccctgcctg agtttaagga tagctctggc ggccctaccg acatcctgag
     4561 aatcggcgag ggctacaaag agaagcagag agccatccag gccgccaaag aggtggaaca
     4621 gagaagagcc ggcggataca gcagaaagtt cgccagcaag tccagaaacc tggccgatga
     4681 catggtcagg aacagcgcca gggacctgtt ctaccacgcc gtgacacatg atgccgtgct
     4741 ggtgttcgag aacctgtcaa gaggcttcgg cagacaggga aaaagaacct tcatgaccga
     4801 gagacagtac acgaagatgg aagattggct caccgccaaa ctggcctacg agggcctgac
     4861 aagcaagacc tacctgtcca agacactggc ccagtacacc tccaagacct gctccaattg
     4921 cggcttcacc atcaccaccg ccgactacga tggaatgctc gtgagactga agaaaacctc
     4981 cgacggctgg gccaccacac tgaacaacaa agagctgaaa gccgagggcc agatcaccta
     5041 ctacaacaga tacaagaggc agaccgtcga gaaagagctg tccgccgaac tcgacagact
     5101 gagcgaggaa tccggcaaca acgacatcag caagtggacc aagggacgca gagatgaggc
     5161 cctgttcctg ctgaagaaga ggttcagcca cagacctgtg caagagcagt ttgtgtgcct
     5221 ggattgcggc cacgaagtgc acgcagatga acaggccgct ctgaatatcg ccagaagctg
     5281 gctgttcctg aacagcaaca gcaccgagtt caagagctac aagagcggca agcagccttt
     5341 cgttggagcc tggcaggcct tctacaagag aaggctcaaa gaagtgtgga agcccaacgc
     5401 caagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag attacaaaga
     5461 cgatgacgat aagggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
     5521 cgaagagaat cctggaccga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5581 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5641 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5701 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5761 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5821 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5881 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca
     5941 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6001 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6061 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6121 caagcccggt gcctgaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     6181 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6241 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6301 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6361 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6421 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6481 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6541 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6601 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6661 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6721 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     6781 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6841 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6901 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6961 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7021 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     7081 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7141 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7201 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7261 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7321 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7381 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7441 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7501 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7561 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7621 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7681 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7741 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7801 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7861 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7921 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7981 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8041 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8101 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     8161 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     8221 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     8281 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     8341 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     8401 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     8461 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     8521 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     8581 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     8641 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8701 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8761 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8821 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8881 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8941 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9001 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9061 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9481 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9721 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10681 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11041 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    11101 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    11161 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    11221 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    11281 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
    11341 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
    11401 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
    11461 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    11521 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
    11581 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
    11641 gcagtacatc tacgtattag tcatcgctat taccatgg
//
