LOCUS       sequence-373712-       10680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi backbone plasmid harboring BbsI sites for easy cloning of 
            sgRNA targeting regions via Golden gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Ameruoso A, Villegas Kcam MC, Cohen KP, Chappell J
  TITLE     Activating natural product synthesis using CRISPR interference and 
            activation systems in Streptomyces.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7751-7760. doi: 
            10.1093/nar/gkac556.
  PUBMED    35801861
REFERENCE   2  (bases 1 to 10680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7751-7760"
COMMENT     Sequence Label: pJEC726
FEATURES             Location/Qualifiers
     source          1..10680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(81..1523)
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            2690..2799
                     /note="incP origin of transfer"
     misc_RNA        complement(3133..3182)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             join(3205..3231,3232..3234,3235..5721,5722..5724,
                     5725..7311)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7608..7904)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     7754..7776
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7768..7785
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7769..7785
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7795..7814
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7795..7813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7861..7881)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7861..7879)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7900..7916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7900..7916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7913..7935)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7924..7940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7948..7954,7955..7972,7973..7978))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7993..8014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        8057..8132
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      8154..8188
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(8230..8818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8310..8329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9022..9822
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
ORIGIN
        1 tcggctttcg cgccgggacc gccatgagac cgccacccgg atgtccgggg tggcggtctc
       61 atggcggtcc ctcagcggcc ctacgcgacc gctgtgtcga cgcggaggca gtctccgggg
      121 tgctggcccc agggctggag taccggggcg agcttgccct tgcagcggcg gcacacgggc
      181 gacgcggcgc cgggcggggg agtcttgacg tcgacggtga ccggcttcgg cttcaggcgc
      241 ttccgaaggc tgatcgtcgg gcggatcgcc tccttcttcg gctgacgcac tcggtcgagc
      301 gaagcggcca gctcctccgt gcgggcctcg ttggccttcc ggcgcgcctc gcggaaagca
      361 gcttcctcct cggacatgac ctcgaacctc atctggtcag cgtcaaggtc gcccggcgcg
      421 gccggcgctt ccggggcggg cgggtccagg acgtccttgc cccacaccag gccccaggac
      481 tcgacgagcc gccggacgcc cggtaggccg tacgtctcgg cgaccttgat gaggtcgagg
      541 cgacgtccgg cgacgcgggc gatgtatcgg taccagatgt aggccgggat gaccgcgatg
      601 gcgaccaggc cctcggtgtc gtcggtgatc tcctcctcgg tgcggacgtc ctgctggatg
      661 ccgagttcct tgatcagccg gttcaggttc tgcgaccggt agtgcttgcg gacctggaac
      721 acgccgaact cgcgctcgcg gtacttctcg acgaacgggc cgggccgacg aagccgctgc
      781 agctcggcgg ccgccgcgtc gcccaggtcg agcggtccca tgcggtcgtc gccgcgaccg
      841 gccttgaagt tctgtccggc cagctccagg ccgatcttgg cgacgccgcc cttggtcttg
      901 tcgccgtcct tgtagaggta gcgggcctgc ttgcccgcat cgccgtcagc ggcgtccgcg
      961 ccgttgagtg ggcgcacgtc ggtgccgtgg cccttgccct cacaggagca accgggccgg
     1021 tcgcacgtct cgctgacggt gtagccgccc gcggattcga ccccggcggc ccaggctccg
     1081 gcgagtgcgt cgcggaacgc ggcctgggcg tccgggccga gcacctcgcg ggtgacccag
     1141 agcgtgtgcc agtgcaggtg ccagccggag ccccagccga aggtgtcctc gaaggcccgc
     1201 tcgtagccga tgatcccgaa gtcgtcgcgc atcgtgcgcc agcggcggcc ggacgagccg
     1261 tacgcgccct tccagccgtc gtgcaagacc gcgaccaggc cgtgccgcat tcccttgcgg
     1321 acggtgccga acgccatgcg ctcgaagtgg cgcaacgtgt tcgtgccaag gtgcagcccg
     1381 tacccggcgt ccgcgagacc gtcggcggcg agctgcacgt tcgagccccg tacggccagg
     1441 atgcggctca tgcaccacgg gcaggtgtgg acgttgttgc agcggcacgt gttgccccac
     1501 gtcgcctcgc ccggcttcca catcagctcg gccgtcccgg cagtgagccg ggtcccgcag
     1561 cccttgaacg cctcgttcag cgacaccgtc tggtgccggt cccgccgggc gaaccgctcg
     1621 tcgcgcgggt cctcccgccc ggctgtcgcg gcaccctcgt ttggggtaga acccgttcca
     1681 gttacagcgc tctgacctgc agtggacgga gattttccct tactactaaa gcccgcgtcc
     1741 ggattacccg ctgtagtcgt gcttgctacg ctgcgtgact ggtccgcaat gagacgcttt
     1801 gcgcgctttc ggcaggcgtc cgagcagtag attttggggc gcttcccggg gatgtggacg
     1861 atcggggtgc cgcagtggca cttcggtccg gcggggcgcg gtggtgtcga cgcgctgttc
     1921 tctcgtacgc tcgtcacaga gcaaacgtcc tcactcggca tgctgcgccg gttcgggggc
     1981 ggcgagcccg ggaggccaat cccgggctcg tgccatttct gggtcctgtt gatcctggca
     2041 ttggtgtggc cgttcattgc ccctgctcgc tcctgacgcg ccgatagacg tccgatacgc
     2101 ccggtgctgg tgggatttga taggtcggaa gaagccccgc ccgggcctgg gcggggcttc
     2161 ctgtgcgtca ggacctcctc gtcgtgagcc tcttcggcct atggacggag tgacctcgtg
     2221 atccgttaca gccgcgcgcg ctcgcgtaga gcggtctcat cagttccacg aacggtcctc
     2281 ttcgcagatc agggcgttgg ggcggagtct caccaaggac tacgtctgct ggcgatttcc
     2341 gttacacccc gggcggtggc cggcgcacac gcgcgcccgc gttgggcagt gcagaaagtg
     2401 cagaaaccta ggcgctgatg gtccaggtcc acggttcgtc gtcggcggcg gcgcgggcgg
     2461 cggcgtcggc cagggcgcgg gcgagaccgg ctacggcggg cttgatgcgc cggttgcggg
     2521 cgaccttgag cagctagtat gcaggtcgac ggatcttttc cgctgcataa ccctgcttcg
     2581 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
     2641 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
     2701 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg
     2761 cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg
     2821 agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcaagg
     2881 tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg gccggcatga
     2941 gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc gtcgtggact
     3001 atgagcacgt ccgcgagctg gacagcgtgc aggactgggg gagttatgtt cacattcgaa
     3061 ccgtctctgc tttgacatcg tgtggcgctt gggtgtaaag tcgtggccat taaacaaaat
     3121 tatttgtaga ggctgtttcg tcctcacgga ctcatcagac cggaaagcac atccggtgac
     3181 agctaactac gaaggggagt cagtatggac aagaagtaca gcatcggcct ggccatcggc
     3241 accaacagcg tgggctgggc ggtcatcacc gacgagtaca aggtcccctc caagaagttc
     3301 aaggtcctgg gcaacaccga ccggcactcg atcaagaaga acctgatcgg cgccctgctc
     3361 ttcgacagcg gcgaaaccgc cgaggcgacc cgcctgaagc ggaccgcccg tcgccgctac
     3421 acccggcgca agaaccgcat ctgctacctg caggagatct tctccaacga gatggccaag
     3481 gtcgacgact cgttcttcca ccggctcgag gagagcttcc tggtggagga ggacaagaag
     3541 cacgagcgcc acccgatctt cggcaacatc gtcgacgagg tggcctacca cgagaagtac
     3601 cccaccatct accacctccg caagaagctg gtcgactcga ccgacaaggc ggacctgcgg
     3661 ctcatctacc tggccctcgc gcacatgatc aagttccgcg gccacttcct catcgagggc
     3721 gacctgaacc cggacaactc cgacgtcgac aagctcttca tccagctggt gcagacctac
     3781 aaccagctgt tcgaggagaa ccccatcaac gccagcggcg tcgacgccaa ggcgatcctc
     3841 tccgcgcgcc tgagcaagtc ccggcgcctg gagaacctca tcgcccagct gccgggcgag
     3901 aagaagaacg gcctcttcgg caacctgatc gcgctgtcgc tcggcctgac ccccaacttc
     3961 aagagcaact tcgacctggc cgaggacgcg aagctccagc tgtccaagga cacctacgac
     4021 gacgacctgg acaacctgct cgcccagatc ggcgaccagt acgcggacct cttcctggcc
     4081 gcgaagaacc tctcggacgc catcctgctc agcgacatcc tgcgggtcaa caccgagatc
     4141 accaaggccc cgctgtcggc gagcatgatc aagcggtacg acgagcacca ccaggacctg
     4201 accctgctca aggccctcgt gcgccagcag ctgcccgaga agtacaagga gatcttcttc
     4261 gaccagtcca agaacggcta cgccggctac atcgacggcg gcgcgtcgca ggaggagttc
     4321 tacaagttca tcaagccgat cctggagaag atggacggca ccgaggagct gctcgtcaag
     4381 ctgaaccgcg aggacctgct ccgcaagcag cggaccttcg acaacggctc catcccgcac
     4441 cagatccacc tgggcgagct ccacgccatc ctccggcgcc aggaggactt ctaccccttc
     4501 ctgaaggaca accgcgagaa gatcgagaag atcctgacct tccggatccc gtactacgtc
     4561 ggccccctgg cccgcggcaa ctcccggttc gcgtggatga cccggaagtc ggaggaaacc
     4621 atcaccccgt ggaacttcga ggaggtcgtg gacaagggcg cctccgcgca gtcgttcatc
     4681 gagcgcatga ccaacttcga caagaacctc ccgaacgaga aggtcctgcc caagcacagc
     4741 ctgctctacg agtacttcac cgtgtacaac gagctgacca aggtcaagta cgtgaccgag
     4801 ggcatgcgga agccggcctt cctgtccggc gagcagaaga aggcgatcgt cgacctgctc
     4861 ttcaagacca accgcaaggt caccgtgaag cagctgaagg aggactactt caagaagatc
     4921 gagtgcttcg actccgtcga gatctcgggc gtggaggacc gcttcaacgc ctccctgggc
     4981 acctaccacg acctgctcaa gatcatcaag gacaaggact tcctcgacaa cgaggagaac
     5041 gaggacatcc tggaggacat cgtcctcacc ctgaccctct tcgaggaccg cgagatgatc
     5101 gaggagcggc tcaagaccta cgcccacctg ttcgacgaca aggtgatgaa gcagctgaag
     5161 cggcgccggt acaccggctg gggccgcctc tcccggaagc tgatcaacgg catccgggac
     5221 aagcagagcg gcaagaccat cctggacttc ctcaagtccg acggcttcgc caaccgcaac
     5281 ttcatgcagc tcatccacga cgactcgctg accttcaagg aggacatcca gaaggcccag
     5341 gtgtccggcc agggcgacag cctccacgag cacatcgcca acctggcggg ctccccggcg
     5401 atcaagaagg gcatcctcca gaccgtcaag gtcgtggacg agctggtcaa ggtgatgggc
     5461 cgccacaagc ccgagaacat cgtgatcgag atggcccggg agaaccagac cacccagaag
     5521 ggccagaaga actcccgcga gcggatgaag cgcatcgagg agggcatcaa ggagctcggc
     5581 tcgcagatcc tgaaggagca cccggtcgag aacacccagc tccagaacga gaagctgtac
     5641 ctctactacc tgcagaacgg ccgcgacatg tacgtggacc aggagctcga catcaaccgg
     5701 ctgagcgact acgacgtcga cgccatcgtg ccgcagtcct tcctgaagga cgactcgatc
     5761 gacaacaagg tcctgacccg ctccgacaag aaccggggca agtccgacaa cgtgccctcg
     5821 gaggaggtcg tgaagaagat gaagaactac tggcgccagc tgctcaacgc caagctcatc
     5881 acccagcgca agttcgacaa cctgaccaag gccgagcggg gcggcctgtc ggagctcgac
     5941 aaggcgggct tcatcaagcg ccagctcgtc gaaacccggc agatcaccaa gcacgtggcc
     6001 cagatcctgg acagccggat gaacaccaag tacgacgaga acgacaagct gatccgcgag
     6061 gtcaaggtga tcaccctcaa gagcaagctg gtgtccgact tccgcaagga cttccagttc
     6121 tacaaggtcc gggagatcaa caactaccac cacgcccacg acgcgtacct gaacgccgtc
     6181 gtgggcaccg cgctgatcaa gaagtacccg aagctggagt ccgagttcgt ctacggcgac
     6241 tacaaggtct acgacgtgcg caagatgatc gccaagtcgg agcaggagat cggcaaggcc
     6301 accgcgaagt acttcttcta cagcaacatc atgaacttct tcaagaccga gatcaccctg
     6361 gccaacggcg agatccgcaa gcggcccctg atcgaaacca acggcgaaac cggcgagatc
     6421 gtctgggaca agggccgcga cttcgccacc gtccggaagg tgctgtccat gccgcaggtc
     6481 aacatcgtca agaaaaccga ggtgcagacc ggcggcttca gcaaggagtc catcctcccc
     6541 aagcgcaact cggacaagct gatcgcccgg aagaaggact gggacccgaa gaagtacggc
     6601 ggcttcgaca gccccaccgt cgcctactcc gtgctggtcg tggcgaaggt cgagaagggc
     6661 aagagcaaga agctgaagtc cgtgaaggag ctgctcggca tcaccatcat ggagcgctcc
     6721 tcgttcgaga agaacccgat cgacttcctg gaggccaagg gctacaagga ggtcaagaag
     6781 gacctcatca tcaagctgcc caagtactcg ctgttcgagc tcgagaacgg ccgcaagcgg
     6841 atgctcgcca gcgcgggcga gctgcagaag ggcaacgagc tggccctccc gtccaagtac
     6901 gtcaacttcc tgtacctcgc gtcccactac gagaagctga agggctcgcc cgaggacaac
     6961 gagcagaagc agctcttcgt ggagcagcac aagcactacc tggacgagat catcgagcag
     7021 atctcggagt tcagcaagcg ggtcatcctg gccgacgcga acctcgacaa ggtgctgtcc
     7081 gcctacaaca agcaccgcga caagccgatc cgggagcagg cggagaacat catccacctg
     7141 ttcaccctca ccaacctggg cgcccccgcc gcgttcaagt acttcgacac caccatcgac
     7201 cgcaagcggt acaccagcac caaggaggtc ctcgacgcga ccctgatcca ccagtccatc
     7261 accggcctgt acgaaacccg catcgacctc tcccagctcg gcggcgactg agaattcaga
     7321 tctacgcgtt ccccgcaaaa gcggcctttg actccctgca agcctcagcg accgaatata
     7381 tcggttatgc gtgggcgatg gttgttgtca ttgtcggcgc aactatcggt atcaagctgt
     7441 ttaagaaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga
     7501 gccttttttt gacagcgtgc aggactgggg gagttatgtt cacattcgaa ccgtctctgc
     7561 tttgacacgg acaagcgcta tggtgtaaag tcgtggccaa agtcttctca gccgctacag
     7621 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     7681 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     7741 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     7801 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcctcca gcttttgttc
     7861 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7921 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7981 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8041 ccaccggtga agacatgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     8101 atcaacttga aaaagtggca ccgagtcggt gctttttact ccatctggat ttgttcagaa
     8161 cgctcggttg ccgccgggcg ttttttatct agaggccagg aaccgtaaaa aggccgcgtt
     8221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8461 cgttcgctcc aagctgggct gtgtgcacga cccccccgtt cagcccgacc gctgcgcctt
     8521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8641 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg ttcatgtgca
     8941 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
     9001 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
     9061 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
     9121 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
     9181 atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
     9241 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
     9301 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
     9361 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac
     9421 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
     9481 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
     9541 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
     9601 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
     9661 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
     9721 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
     9781 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
     9841 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat
     9901 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc
     9961 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg
    10021 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
    10081 tatcgatgtc gaggttcctc aggggagcca ccccagagaa gccctcggag ctgagcggag
    10141 ctatttccaa agccatgcca gctagagaca gtgcacaccg ccaagcatct gcaaaaccct
    10201 cgcctggagg gaaagtgcaa tgtacgtacg ctggtttccc tccagaaagg gattctgcgg
    10261 cttctcaact ttggaagaag aggcggaacg aactgctgtt tcagcctact catgtgagga
    10321 ggctggtcct ttaccctgat gcccgagggc atcagggtaa aggaccagcc tcgccaacta
    10381 ggagcgctac caaggcgaac acaacccagg acttggtaga acccttcgcc ggagaagttc
    10441 agactcatcg gcatgagcac ccctcgccgc gcactcggca ccggtccgtc gaccaccacc
    10501 aggacgtcgt tgtcgacgtc ggccccgcgg ctcctgcccg ccgaacgcgt cgtcgtcgac
    10561 ggcctggtgc tcatcgacga gcacccggag ccaggtgaaa agcgccggcg gacgctcgga
    10621 ctgggcgcgg gattccagca gtaacccagg tccgccggcc acctcacggc aggcagaccc
//
