LOCUS       sequence-373713-        8261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the mouse BDNF tagged with the FP pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8261)
  TITLE     Neurotrophin related plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-BDNF-pHluorin
FEATURES             Location/Qualifiers
     source          1..8261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     620..639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(858..962)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(988..1443)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1125..1146)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1337..1356
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1570..1592
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1584..1601
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1585..1601
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1611..1630
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1611..1629
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      1650..1785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1825..1946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1836..1855)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1890..1909
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2018..2251
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2338..2926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2444..2455
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2853..2873
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     3380..3399
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(4562..4586)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4583..4786)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4612..4632)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4787..5090
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    5113..5229
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5496..5540)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5725..5958
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6451..6576
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6623..6803
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6804..7030)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(7058..7078)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7058..7076)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7097..7113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7097..7113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7110..7132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7121..7137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7145..7151,7152..7169,7170..7175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7190..7211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7328..7345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7499..8087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7579..7598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8258..8261,1..788,789..857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
       61 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      541 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      601 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      661 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      721 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      781 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      841 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      901 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      961 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     1021 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     1081 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     1141 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     1201 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     1261 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     1321 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     1381 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     1441 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1501 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     1561 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     1621 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     1681 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     1741 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     1801 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     1861 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     1921 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     1981 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     2041 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     2101 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     2161 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     2221 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     2281 agatctacag ctgccttgta agtcattggt cttaaaggta ccgagctcga attccaggcg
     2341 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     2401 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     2461 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccatggaa
     2521 aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     2581 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     2641 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     2701 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     2761 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     2821 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     2881 gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg atatctgtac
     2941 agggccctga tcatccggag tcgaccctgc aggtctagag ctagctcatt tgtacagctc
     3001 gtccatgccg tgggtgatgc cagcggcggt cacgaattcc agcagcacca tgtggtccct
     3061 cttctcgttg gggtccttgc tcagggtgct ggtggtgaac aggtagtggt tgtcaggcag
     3121 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcca gctgcacgcc
     3181 gccgtcctcg atgttgtgcc tgatcttgaa gttggccttg atgccgttct tctgcttgtc
     3241 ggccatgatg tacacctggt gatcgttgta gttgtattcg agcttgtggc ccaggatgtt
     3301 gccatcctct ttgaagtcga tgcccttcag ctcgattctg ttgaccagtg tgtcgccctc
     3361 gaacttcact tcggctctgg tcttgtagtt gccgtcgtcc ttgaagaaga tggttctttc
     3421 ctgcacgtag ccctcgggca tggcgctctt gaagaaatcg tgcctcttca tgtggtcggg
     3481 gtatctgctg aagcactgca cgccgtaggt cagggttgtg accagggtgg gccaaggcac
     3541 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtatg tggcgtcccc
     3601 ctcgccctcg ccggacacgc tgaacttgtg tccgttcacg tcgccgtcca gttccaccag
     3661 gatgggcacc acgccggtga acagttcctc gcccttagac attccgccgg tgccgccaga
     3721 gccgccggat ctgcctctct tgatggtcag ggtgcacacg cagctggtgt cgattctgat
     3781 gaaccgccag ccgattctct tcttgctgtc catggtcagg gcccgcacgt agctctgggt
     3841 ggttctgcac tggctgttcc agtgtctctt gtcgatgcct ctgcagccct ctttggtgta
     3901 gcccatgggg ttgcactttg tctcgtagaa gtactgcttc agctggccct tggacacggg
     3961 caccttttcc agcacggtca cggtgccgcc gctcatgtcc acggcggttt tcttgtcggc
     4021 ggctgtgacc cactcgctga tgctgtcaca cacgctcagc tcgccccgtc tggcggggtc
     4081 gctgtgtctc cgcactctca tgctcatgtt ggcggcgtcc aggtagttct tgtactcttc
     4141 cagcaggaac agcagggggg gttccagggg cacctggctg ctcagcatca ctctgctggt
     4201 gtacaggtcg gcgtccttgt ggttttcctc gttgggccgc actttctggt cctcgtccag
     4261 cagttcctcg atcacgtgct cgaaggtgtc ggccaggctg gttgtggtca ggcctctgct
     4321 gccagctctg gggccgttca cgctttccag ggtgccgtgg gtccgcacgc cagggtaggc
     4381 caggttgccc tggccgtgga cgttgacttc cttcatggga gcggccttca tgcagccgaa
     4441 gtagctgatg accatggtca gaaacaggat ggtcatggat ccctcgagca ccggtgggat
     4501 cccgtacgcc cgggcggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
     4561 cgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac
     4621 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     4681 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     4741 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     4801 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     4861 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     4921 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     4981 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     5041 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactccc
     5101 aagcttatcg ataaaatttt gaattttgta atttgttttt gtaattcttt agtttgtatg
     5161 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     5221 cttttaaaag ttaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     5281 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     5341 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     5401 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     5461 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     5521 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     5581 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     5641 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     5701 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     5761 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     5821 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     5881 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     5941 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     6001 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     6061 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     6121 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     6181 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     6241 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     6301 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     6361 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     6421 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     6481 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     6541 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     6601 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     6661 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     6721 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     6781 atctggtcta accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct
     6841 ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct
     6901 aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct
     6961 ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca
     7021 taagactaca ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc
     7081 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7141 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7201 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7921 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8221 caatctaaag tatatatgag taaacttggt ctgacagtta c
//
