LOCUS       sequence-373716-        8769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the Human TrkB receptor under the Moloney mouse sarcoma 
            virus LTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Urfer R, Tsoulfas P, O'Connell L, Hongo JA, Zhao W, Presta LG
  TITLE     High resolution mapping of the binding site of TrkA for nerve growth
            factor and TrkC for neurotrophin-3 on the second immunoglobulin-like
            domain of the Trk receptors.
  JOURNAL   J Biol Chem. 1998 Mar 6;273(10):5829-40. doi: 
            10.1074/jbc.273.10.5829.
  PUBMED    9488719
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.273.10"; journalName: "J Biol Chem"; date: "1998-03-6- 
            6"; volume: "273"; issue: "10"; pages: "5829-40"
COMMENT     Sequence Label: pMEXneo-TrkB
FEATURES             Location/Qualifiers
     source          1..8769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(245..349)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     417..435
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     808..830
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     822..839
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     823..839
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4051..4185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4075..4094)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4129..4148
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4610..4630)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4760..4825
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(5245..6039)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5356..5375)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5966..5985
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6396..6725)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6410..6545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6710..6730
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(6992..7008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6992..7008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7005..7027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7016..7032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7040..7046,7047..7064,7065..7070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7085..7106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7223..7240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7394..7982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7474..7493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8153..8769,1..175,176..244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
       61 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
      121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      361 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      421 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
      481 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
      541 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
      601 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
      661 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
      721 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
      781 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      841 ccaagctaat tctctgtact ctatatttaa gtttcctata aatctatagc ttttctaaaa
      901 ataaaatcta tttaaaaatt acttgaatga aaggtaaaca cgcctctttc acacagtctg
      961 atgcaaagtc tcatcaagaa ttgggcagtc ctgggaagtt ggaaccctgt aaatacggac
     1021 tccaccgtgc agacaatgcc atgtagagaa gcgtgttccc aagatcccag cttccccact
     1081 ctgggtcctt cccctcatag atataaacgt gaaagacccc agccgtaggt ttggcaagct
     1141 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     1201 cagatcaagg tcaggaacag agagacagct gaatatgggc caaacaggat atctgtggta
     1261 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaatt ggggcaaaca
     1321 ggatatctgc ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     1381 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     1441 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     1501 tcgcgcgctt ccgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     1561 agtcttccga tagactgcgt cgcccagctt gcatgcctgc aggtcgacat ggcgcggctc
     1621 tggggcttct gctggctggt tgtgggcttc tggagggccg ctttcgcctg tcccacgtcc
     1681 tgcaaatgca gtgcctctcg gatctggtgc agcgaccctt ctcctggcat cgtggcattt
     1741 ccgagattgg agcctaacag tgtagatcct gagaacatca ccgaaatttt catcgcaaac
     1801 cagaaaaggt tagaaatcat caacgaagat gatgttgaag cttatgtggg actgagaaat
     1861 ctgacaattg tggattctgg attaaaattt gtggctcata aagcatttct gaaaaacagc
     1921 aacctgcagc acatcaattt tacccgaaac aaactgacga gtttgtctag gaaacatttc
     1981 cgtcaccttg acttgtctga actgatcctg gtgggcaatc catttacatg ctcctgtgac
     2041 attatgtgga tcaagactct ccaagaggct aaatccagtc cagacactca ggatttgtac
     2101 tgcctgaatg aaagcagcaa gaatattccc ctggcaaacc tgcagatacc caattgtggt
     2161 ttgccatctg caaatctggc cgcacctaac ctcactgtgg aggaaggaaa gtctatcaca
     2221 ttatcctgta gtgtggcagg tgatccggtt cctaatatgt attgggatgt tggtaacctg
     2281 gtttccaaac atatgaatga aacaagccac acacagggct ccttaaggat aactaacatt
     2341 tcatccgatg acagtgggaa gcagatctct tgtgtggcgg aaaatcttgt aggagaagat
     2401 caagattctg tcaacctcac tgtgcatttt gcaccaacta tcacatttct cgaatctcca
     2461 acctcagacc accactggtg cattccattc actgtgaaag gcaacccaaa accagcgctt
     2521 cagtggttct ataacggggc aatattgaat gagtccaaat acatctgtac taaaatacat
     2581 gttaccaatc acacggagta ccacggctgc ctccagctgg ataatcccac tcacatgaac
     2641 aatggggact acactctaat agccaagaat gagtatggga aggatgagaa acagatttct
     2701 gctcacttca tgggctggcc tggaattgac gatggtgcaa acccaaatta tcctgatgta
     2761 atttatgaag attatggaac tgcagcgaat gacatcgggg acaccacgaa cagaagtaat
     2821 gaaatccctt ccacagacgt cactgataaa accggtcggg aacatctctc ggtctatgct
     2881 gtggtggtga ttgcgtctgt ggtgggattt tgccttttgg taatgctgtt tctgcttaag
     2941 ttggcaagac actccaagtt tggcatgaaa ggcccagcct ccgttatcag caatgatgat
     3001 gactctgcca gcccactcca tcacatctcc aatgggagta acactccatc ttcttcggaa
     3061 ggtggcccag atgctgtcat tattggaatg accaagatcc ctgtcattga aaatccccag
     3121 tactttggca tcaccaacag tcagctcaag ccagacacat ttgttcagca catcaagcga
     3181 cataacattg ttctgaaaag ggagctaggc gaaggagcct ttggaaaagt gttcctagct
     3241 gaatgctata acctctgtcc tgagcaggac aagatcttgg tggcagtgaa gaccctgaag
     3301 gatgccagtg acaatgcacg caaggacttc caccgtgagg ccgagctcct gaccaacctc
     3361 cagcatgagc acatcgtcaa gttctatggc gtctgcgtgg agggcgaccc cctcatcatg
     3421 gtctttgagt acatgaagca tggggacctc aacaagttcc tcagggcaca cggccctgat
     3481 gccgtgctga tggctgaggg caacccgccc acggaactga cgcagtcgca gatgctgcat
     3541 atagcccagc agatcgccgc gggcatggtc tacctggcgt cccagcactt cgtgcaccgc
     3601 gatttggcca ccaggaactg cctggtcggg gagaacttgc tggtgaaaat cggggacttt
     3661 gggatgtccc gggacgtgta cagcactgac tactacaggg tcggtggcca cacaatgctg
     3721 cccattcgct ggatgcctcc agagagcatc atgtacagga aattcacgac ggaaagcgac
     3781 gtctggagcc tgggggtcgt gttgtgggag attttcacct atggcaaaca gccctggtac
     3841 cagctgtcaa acaatgaggt gatagagtgt atcactcagg gccgagtcct gcagcgaccc
     3901 cgcacgtgcc cccaggaggt gtatgagctg atgctggggt gctggcagcg agagccccac
     3961 atgaggaaga acatcaaggg catccatacc ctccttcaga acttggccaa ggcatctccg
     4021 gtctacctgg acattctagg ctagaattcc gatccagaca tgataagata cattgatgag
     4081 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4141 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4201 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     4261 ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt
     4321 ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat
     4381 taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac
     4441 tgttatgcct acttataaag gttacagaat atttttccat aattttcttg tatagcagtg
     4501 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
     4561 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
     4621 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
     4681 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
     4741 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
     4801 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
     4861 attatgtcac accacagaag taaggttcct tcacaaagat ccggaccaaa gcggccatcg
     4921 tgcctcccca ctcctgcagt tcgggggcat ggatgcgcgg atagccgctg ctggtttcct
     4981 ggatgccgac ggatttgcac tgccggtaga actccgcgag gtcgtccagc ctcaggcagc
     5041 agctgaacca actcgcgagg ggatcgagcc cggggtgggc gaagaactcc agcatgagat
     5101 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     5161 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     5221 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     5281 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     5341 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     5401 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     5461 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     5521 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     5581 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     5641 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     5701 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     5761 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     5821 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     5881 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     5941 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     6001 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     6061 ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     6121 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc
     6181 gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac
     6241 ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     6301 ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc
     6361 cttgcgccct gagtgcttgc ggcagcgtga agctttttgc aaaagcctag gcctccaaaa
     6421 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
     6481 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     6541 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     6601 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     6661 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     6721 cacacattcc acagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6781 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6841 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     6901 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6961 accatacgga tcggaattaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     7021 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7081 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7141 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7201 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7801 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8041 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8101 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8461 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8761 cggcgtcaa
//
