LOCUS       sequence-373717-       11420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRa backbone plasmid harboring BbsI sites for easy cloning of 
            sgRNA targeting regions via Golden gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Ameruoso A, Villegas Kcam MC, Cohen KP, Chappell J
  TITLE     Activating natural product synthesis using CRISPR interference and 
            activation systems in Streptomyces.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7751-7760. doi: 
            10.1093/nar/gkac556.
  PUBMED    35801861
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7751-7760"
COMMENT     Sequence Label: pJEC819
FEATURES             Location/Qualifiers
     source          1..11420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(300..1742)
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
     oriT            2909..3018
                     /note="incP origin of transfer"
     misc_RNA        complement(3352..3401)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             join(3424..3450,3451..3453,3454..5940,5941..5943,
                     5944..7527)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8567..8863)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     8713..8735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8727..8744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8728..8744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8754..8773
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8754..8772
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8820..8840)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8820..8838)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8859..8875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8859..8875)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8872..8894)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8883..8899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8907..8913,8914..8931,8932..8937))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8952..8973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        9016..9091
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      9113..9147
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(9189..9777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9269..9288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9981..10781
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
ORIGIN
        1 cctcgccgcg cactcggcac cggtccgtcg accaccacca ggacgtcgtt gtcgacgtcg
       61 gccccgcggc tcctgcccgc cgaacgcgtc gtcgtcgacg gcctggtgct catcgacgag
      121 cacccggagc caggtgaaaa gcgccggcgg acgctcggac tgggcgcggg attccagcag
      181 taacccaggt ccgccggcca cctcacggca ggcagaccct cggctttcgc gccgggaccg
      241 ccatgagacc gccacccgga tgtccggggt ggcggtctca tggcggtccc tcagcggccc
      301 tacgcgaccg ctgtgtcgac gcggaggcag tctccggggt gctggcccca gggctggagt
      361 accggggcga gcttgccctt gcagcggcgg cacacgggcg acgcggcgcc gggcggggga
      421 gtcttgacgt cgacggtgac cggcttcggc ttcaggcgct tccgaaggct gatcgtcggg
      481 cggatcgcct ccttcttcgg ctgacgcact cggtcgagcg aagcggccag ctcctccgtg
      541 cgggcctcgt tggccttccg gcgcgcctcg cggaaagcag cttcctcctc ggacatgacc
      601 tcgaacctca tctggtcagc gtcaaggtcg cccggcgcgg ccggcgcttc cggggcgggc
      661 gggtccagga cgtccttgcc ccacaccagg ccccaggact cgacgagccg ccggacgccc
      721 ggtaggccgt acgtctcggc gaccttgatg aggtcgaggc gacgtccggc gacgcgggcg
      781 atgtatcggt accagatgta ggccgggatg accgcgatgg cgaccaggcc ctcggtgtcg
      841 tcggtgatct cctcctcggt gcggacgtcc tgctggatgc cgagttcctt gatcagccgg
      901 ttcaggttct gcgaccggta gtgcttgcgg acctggaaca cgccgaactc gcgctcgcgg
      961 tacttctcga cgaacgggcc gggccgacga agccgctgca gctcggcggc cgccgcgtcg
     1021 cccaggtcga gcggtcccat gcggtcgtcg ccgcgaccgg ccttgaagtt ctgtccggcc
     1081 agctccaggc cgatcttggc gacgccgccc ttggtcttgt cgccgtcctt gtagaggtag
     1141 cgggcctgct tgcccgcatc gccgtcagcg gcgtccgcgc cgttgagtgg gcgcacgtcg
     1201 gtgccgtggc ccttgccctc acaggagcaa ccgggccggt cgcacgtctc gctgacggtg
     1261 tagccgcccg cggattcgac cccggcggcc caggctccgg cgagtgcgtc gcggaacgcg
     1321 gcctgggcgt ccgggccgag cacctcgcgg gtgacccaga gcgtgtgcca gtgcaggtgc
     1381 cagccggagc cccagccgaa ggtgtcctcg aaggcccgct cgtagccgat gatcccgaag
     1441 tcgtcgcgca tcgtgcgcca gcggcggccg gacgagccgt acgcgccctt ccagccgtcg
     1501 tgcaagaccg cgaccaggcc gtgccgcatt cccttgcgga cggtgccgaa cgccatgcgc
     1561 tcgaagtggc gcaacgtgtt cgtgccaagg tgcagcccgt acccggcgtc cgcgagaccg
     1621 tcggcggcga gctgcacgtt cgagccccgt acggccagga tgcggctcat gcaccacggg
     1681 caggtgtgga cgttgttgca gcggcacgtg ttgccccacg tcgcctcgcc cggcttccac
     1741 atcagctcgg ccgtcccggc agtgagccgg gtcccgcagc ccttgaacgc ctcgttcagc
     1801 gacaccgtct ggtgccggtc ccgccgggcg aaccgctcgt cgcgcgggtc ctcccgcccg
     1861 gctgtcgcgg caccctcgtt tggggtagaa cccgttccag ttacagcgct ctgacctgca
     1921 gtggacggag attttccctt actactaaag cccgcgtccg gattacccgc tgtagtcgtg
     1981 cttgctacgc tgcgtgactg gtccgcaatg agacgctttg cgcgctttcg gcaggcgtcc
     2041 gagcagtaga ttttggggcg cttcccgggg atgtggacga tcggggtgcc gcagtggcac
     2101 ttcggtccgg cggggcgcgg tggtgtcgac gcgctgttct ctcgtacgct cgtcacagag
     2161 caaacgtcct cactcggcat gctgcgccgg ttcgggggcg gcgagcccgg gaggccaatc
     2221 ccgggctcgt gccatttctg ggtcctgttg atcctggcat tggtgtggcc gttcattgcc
     2281 cctgctcgct cctgacgcgc cgatagacgt ccgatacgcc cggtgctggt gggatttgat
     2341 aggtcggaag aagccccgcc cgggcctggg cggggcttcc tgtgcgtcag gacctcctcg
     2401 tcgtgagcct cttcggccta tggacggagt gacctcgtga tccgttacag ccgcgcgcgc
     2461 tcgcgtagag cggtctcatc agttccacga acggtcctct tcgcagatca gggcgttggg
     2521 gcggagtctc accaaggact acgtctgctg gcgatttccg ttacaccccg ggcggtggcc
     2581 ggcgcacacg cgcgcccgcg ttgggcagtg cagaaagtgc agaaacctag gcgctgatgg
     2641 tccaggtcca cggttcgtcg tcggcggcgg cgcgggcggc ggcgtcggcc agggcgcggg
     2701 cgagaccggc tacggcgggc ttgatgcgcc ggttgcgggc gaccttgagc agctagtatg
     2761 caggtcgacg gatcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     2821 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     2881 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
     2941 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
     3001 ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg
     3061 aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac
     3121 gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg
     3181 ccgtcggcca gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg
     3241 acagcgtgca ggactggggg agttatgttc acattcgaac cgtctctgct ttgacatcgt
     3301 gtggcgcttg ggtgtaaagt cgtggccatt aaacaaaatt atttgtagag gctgtttcgt
     3361 cctcacggac tcatcagacc ggaaagcaca tccggtgaca gctaactacg aaggggagtc
     3421 agtatggaca agaagtacag catcggcctg gccatcggca ccaacagcgt gggctgggcg
     3481 gtcatcaccg acgagtacaa ggtcccctcc aagaagttca aggtcctggg caacaccgac
     3541 cggcactcga tcaagaagaa cctgatcggc gccctgctct tcgacagcgg cgaaaccgcc
     3601 gaggcgaccc gcctgaagcg gaccgcccgt cgccgctaca cccggcgcaa gaaccgcatc
     3661 tgctacctgc aggagatctt ctccaacgag atggccaagg tcgacgactc gttcttccac
     3721 cggctcgagg agagcttcct ggtggaggag gacaagaagc acgagcgcca cccgatcttc
     3781 ggcaacatcg tcgacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctccgc
     3841 aagaagctgg tcgactcgac cgacaaggcg gacctgcggc tcatctacct ggccctcgcg
     3901 cacatgatca agttccgcgg ccacttcctc atcgagggcg acctgaaccc ggacaactcc
     3961 gacgtcgaca agctcttcat ccagctggtg cagacctaca accagctgtt cgaggagaac
     4021 cccatcaacg ccagcggcgt cgacgccaag gcgatcctct ccgcgcgcct gagcaagtcc
     4081 cggcgcctgg agaacctcat cgcccagctg ccgggcgaga agaagaacgg cctcttcggc
     4141 aacctgatcg cgctgtcgct cggcctgacc cccaacttca agagcaactt cgacctggcc
     4201 gaggacgcga agctccagct gtccaaggac acctacgacg acgacctgga caacctgctc
     4261 gcccagatcg gcgaccagta cgcggacctc ttcctggccg cgaagaacct ctcggacgcc
     4321 atcctgctca gcgacatcct gcgggtcaac accgagatca ccaaggcccc gctgtcggcg
     4381 agcatgatca agcggtacga cgagcaccac caggacctga ccctgctcaa ggccctcgtg
     4441 cgccagcagc tgcccgagaa gtacaaggag atcttcttcg accagtccaa gaacggctac
     4501 gccggctaca tcgacggcgg cgcgtcgcag gaggagttct acaagttcat caagccgatc
     4561 ctggagaaga tggacggcac cgaggagctg ctcgtcaagc tgaaccgcga ggacctgctc
     4621 cgcaagcagc ggaccttcga caacggctcc atcccgcacc agatccacct gggcgagctc
     4681 cacgccatcc tccggcgcca ggaggacttc taccccttcc tgaaggacaa ccgcgagaag
     4741 atcgagaaga tcctgacctt ccggatcccg tactacgtcg gccccctggc ccgcggcaac
     4801 tcccggttcg cgtggatgac ccggaagtcg gaggaaacca tcaccccgtg gaacttcgag
     4861 gaggtcgtgg acaagggcgc ctccgcgcag tcgttcatcg agcgcatgac caacttcgac
     4921 aagaacctcc cgaacgagaa ggtcctgccc aagcacagcc tgctctacga gtacttcacc
     4981 gtgtacaacg agctgaccaa ggtcaagtac gtgaccgagg gcatgcggaa gccggccttc
     5041 ctgtccggcg agcagaagaa ggcgatcgtc gacctgctct tcaagaccaa ccgcaaggtc
     5101 accgtgaagc agctgaagga ggactacttc aagaagatcg agtgcttcga ctccgtcgag
     5161 atctcgggcg tggaggaccg cttcaacgcc tccctgggca cctaccacga cctgctcaag
     5221 atcatcaagg acaaggactt cctcgacaac gaggagaacg aggacatcct ggaggacatc
     5281 gtcctcaccc tgaccctctt cgaggaccgc gagatgatcg aggagcggct caagacctac
     5341 gcccacctgt tcgacgacaa ggtgatgaag cagctgaagc ggcgccggta caccggctgg
     5401 ggccgcctct cccggaagct gatcaacggc atccgggaca agcagagcgg caagaccatc
     5461 ctggacttcc tcaagtccga cggcttcgcc aaccgcaact tcatgcagct catccacgac
     5521 gactcgctga ccttcaagga ggacatccag aaggcccagg tgtccggcca gggcgacagc
     5581 ctccacgagc acatcgccaa cctggcgggc tccccggcga tcaagaaggg catcctccag
     5641 accgtcaagg tcgtggacga gctggtcaag gtgatgggcc gccacaagcc cgagaacatc
     5701 gtgatcgaga tggcccggga gaaccagacc acccagaagg gccagaagaa ctcccgcgag
     5761 cggatgaagc gcatcgagga gggcatcaag gagctcggct cgcagatcct gaaggagcac
     5821 ccggtcgaga acacccagct ccagaacgag aagctgtacc tctactacct gcagaacggc
     5881 cgcgacatgt acgtggacca ggagctcgac atcaaccggc tgagcgacta cgacgtcgac
     5941 gccatcgtgc cgcagtcctt cctgaaggac gactcgatcg acaacaaggt cctgacccgc
     6001 tccgacaaga accggggcaa gtccgacaac gtgccctcgg aggaggtcgt gaagaagatg
     6061 aagaactact ggcgccagct gctcaacgcc aagctcatca cccagcgcaa gttcgacaac
     6121 ctgaccaagg ccgagcgggg cggcctgtcg gagctcgaca aggcgggctt catcaagcgc
     6181 cagctcgtcg aaacccggca gatcaccaag cacgtggccc agatcctgga cagccggatg
     6241 aacaccaagt acgacgagaa cgacaagctg atccgcgagg tcaaggtgat caccctcaag
     6301 agcaagctgg tgtccgactt ccgcaaggac ttccagttct acaaggtccg ggagatcaac
     6361 aactaccacc acgcccacga cgcgtacctg aacgccgtcg tgggcaccgc gctgatcaag
     6421 aagtacccga agctggagtc cgagttcgtc tacggcgact acaaggtcta cgacgtgcgc
     6481 aagatgatcg ccaagtcgga gcaggagatc ggcaaggcca ccgcgaagta cttcttctac
     6541 agcaacatca tgaacttctt caagaccgag atcaccctgg ccaacggcga gatccgcaag
     6601 cggcccctga tcgaaaccaa cggcgaaacc ggcgagatcg tctgggacaa gggccgcgac
     6661 ttcgccaccg tccggaaggt gctgtccatg ccgcaggtca acatcgtcaa gaaaaccgag
     6721 gtgcagaccg gcggcttcag caaggagtcc atcctcccca agcgcaactc ggacaagctg
     6781 atcgcccgga agaaggactg ggacccgaag aagtacggcg gcttcgacag ccccaccgtc
     6841 gcctactccg tgctggtcgt ggcgaaggtc gagaagggca agagcaagaa gctgaagtcc
     6901 gtgaaggagc tgctcggcat caccatcatg gagcgctcct cgttcgagaa gaacccgatc
     6961 gacttcctgg aggccaaggg ctacaaggag gtcaagaagg acctcatcat caagctgccc
     7021 aagtactcgc tgttcgagct cgagaacggc cgcaagcgga tgctcgccag cgcgggcgag
     7081 ctgcagaagg gcaacgagct ggccctcccg tccaagtacg tcaacttcct gtacctcgcg
     7141 tcccactacg agaagctgaa gggctcgccc gaggacaacg agcagaagca gctcttcgtg
     7201 gagcagcaca agcactacct ggacgagatc atcgagcaga tctcggagtt cagcaagcgg
     7261 gtcatcctgg ccgacgcgaa cctcgacaag gtgctgtccg cctacaacaa gcaccgcgac
     7321 aagccgatcc gggagcaggc ggagaacatc atccacctgt tcaccctcac caacctgggc
     7381 gcccccgccg cgttcaagta cttcgacacc accatcgacc gcaagcggta caccagcacc
     7441 aaggaggtcc tcgacgcgac cctgatccac cagtccatca ccggcctgta cgaaacccgc
     7501 atcgacctct cccagctcgg cggcgacagt ggctcagaga cgccgggtac ttctgagtcc
     7561 gctacgcctg agagcatgct tatcgctcag cgtccttcgc tgaccgaaga ggtcgtcgac
     7621 gagttccgct cccggttcgt gatcgagccg ctggagccgg gcttcggcta caccctcggc
     7681 aactccctcc gccgtaccct cctctcctcg atcccgggtg ccgctgtcac cagcatccgc
     7741 atcgacggtg tcctgcacga gttcaccacc gtgccgggcg tcaaggagga cgtcaccgac
     7801 ctcatcctca acatcaagca gctggtcgtc tcctcggagc acgacgagcc ggtcgtgatg
     7861 tacctgcgca agcagggccc gggtctggtc accgccgccg acatcgcgcc cccggccggt
     7921 gtcgaggtgc acaaccccga cctcgtcctc gccacgctca acggcaaggg caagctggag
     7981 atggagctga ccgtcgagcg cggtcgcggc tacgtctccg ccgtgcagaa caagcaggtc
     8041 ggtcaggaga tcgggcgcat cccggtcgac tcgatctact cgccggttct caaggtcacc
     8101 tacaaggtcg aggcgacccg agtcgagcag cgcaccgact tcgacaagct gatcgtcgac
     8161 gtcgagacca agcaggccat gcgcccgcgt gacgccatgg cgtccgccgg caagaccctg
     8221 gtcgagctgt tcggtctggc gcgcgagctc aacatcgacg cctaagttta gaattcagat
     8281 ctacgcgttc cccgcaaaag cggcctttga ctccctgcaa gcctcagcga ccgaatatat
     8341 cggttatgcg tgggcgatgg ttgttgtcat tgtcggcgca actatcggta tcaagctgtt
     8401 taagaaattc acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag
     8461 cctttttttg acagcgtgca ggactggggg agttatgttc acattcgaac cgtctctgct
     8521 ttgacacgga caagcgctat ggtgtaaagt cgtggccaaa gtcttctcag ccgctacagg
     8581 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8641 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8701 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     8761 actcactata gggcgaattg ggtaccgggc cccccctcga ggtcctccag cttttgttcc
     8821 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     8881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8941 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9001 caccggtgaa gacatgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     9061 tcaacttgaa aaagtggcac cgagtcggtg ctttttactc catctggatt tgttcagaac
     9121 gctcggttgc cgccgggcgt tttttatcta gaggccagga accgtaaaaa ggccgcgttg
     9181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9421 gttcgctcca agctgggctg tgtgcacgac ccccccgttc agcccgaccg ctgcgcctta
     9481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9601 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttggt tcatgtgcag
     9901 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt
     9961 gatcgtgcta tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat
    10021 ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc
    10081 tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga
    10141 tcgaggccct gcgtgctgcg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag
    10201 gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg
    10261 tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg
    10321 cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg ccacctcact
    10381 cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg
    10441 tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg
    10501 gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg
    10561 agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc
    10621 ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg
    10681 tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg
    10741 cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa
    10801 cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgt ggagcggatc
    10861 ggggattgtc tttcttcagc tcgctgatga tatgctgacg ctcaatgccg tttggcctcc
    10921 gactaacgaa aatcccgcat ttggacggct gatccgattg gcacggcgga cggcgaatgg
    10981 cggagcagac gctcgtccgg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt
    11041 atcgatgtcg aggttcctca ggggagccac cccagagaag ccctcggagc tgagcggagc
    11101 tatttccaaa gccatgccag ctagagacag tgcacaccgc caagcatctg caaaaccctc
    11161 gcctggaggg aaagtgcaat gtacgtacgc tggtttccct ccagaaaggg attctgcggc
    11221 ttctcaactt tggaagaaga ggcggaacga actgctgttt cagcctactc atgtgaggag
    11281 gctggtcctt taccctgatg cccgagggca tcagggtaaa ggaccagcct cgccaactag
    11341 gagcgctacc aaggcgaaca caacccagga cttggtagaa cccttcgccg gagaagttca
    11401 gactcatcgg catgagcacc
//
