LOCUS       sequence-373724-        1288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of 
            the His8 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Selig EE, Bhura R, White MR, Akula S, Hoffman RD, Tovar CN, Xu X, 
            Booth RE, Libich DS
  TITLE     Biochemical and biophysical characterization of the nucleic acid 
            binding properties of the RNA/DNA binding protein EWS.
  JOURNAL   Biopolymers. 2023 May;114(5):e23536. doi: 10.1002/bip.23536. Epub 
            2023 Mar 17.
  PUBMED    36929870
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biopolymers."; date: "2023-05"; volume: "114(5):e23536. doi"; 
            pages: " 10.1002/bip.23536. Epub 2023 Mar 17"
COMMENT     Sequence Label: pAG8Ha-EWS_RRM-RGG(359-513)
FEATURES             Location/Qualifiers
     source          1..1288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(354..458)
                     /gene="bla"
                     /label=AmpR promoter
     gap             571..609
                     /estimated_length=39
     gap             615..779
                     /estimated_length=165
ORIGIN
        1 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
       61 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
      121 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      181 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      241 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      301 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      361 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      421 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      481 cctgacgyss mcggwwssgg mgmwykawsy scsgatccyc trgkgkykac kckcagtrya
      541 atckgctctg atgccgcata gttaagccag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnt gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      781 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      841 tagatccttt taaattaaaa atgaagtttt aaatcaatct aargtatata tgagtaaact
      901 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      961 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1021 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1081 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1141 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1201 agtttgcgca acgttgttgc cattgctrca ggcatcgtgg tgtcacgctc gtcgtttggt
     1261 atggcttcat tcagctccgg ttcccaac
//
