LOCUS       sequence-373727-       11920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible lentivirus vector to express 
            SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, 
            in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11920)
  AUTHORS   Meiring JCM, Grigoriev I, Nijenhuis W, Kapitein LC, Akhmanova A
  TITLE     Opto-katanin, an optogenetic tool for localized, microtubule 
            disassembly.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4660-4674.e6. doi: 
            10.1016/j.cub.2022.09.010. Epub 2022 Sep 28.
  PUBMED    36174574
REFERENCE   2  (bases 1 to 11920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.010"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4660-4674.e6"
COMMENT     Sequence Label: pSIN-TRE-SspB-mCh-p60
FEATURES             Location/Qualifiers
     source          1..11920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(1954..1972,1973..1995,1996..2014,2015..2037,2038..2056,
                     2057..2079,2080..2098,2099..2121,2122..2140,2141..2163,
                     2164..2182,2183..2205,2206..2224)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2257..2295
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2292..2316
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2470..2676,2677..2679,2680..2799)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             2908..3612
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3050..3068)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        5247..5757
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5676..5694
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(5795..6412,6413..6418,6419..6454,6455..6457,6458..6493,
                     6494..6496,6497..6532,6533..6541)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(6550..7124,7125..7127,7128..7136)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6717..6734)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6944..6963
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7156..7755
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7156..7175)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7652..7672
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7787..8375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7840..7860)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8258..8269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8462..8695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8767..8888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8804..8823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8858..8877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8907..9042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9062..9081)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9063..9081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9091..9108)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9091..9107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9100..9122)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9249..9704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9336..9355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9546..9567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9730..9834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9835..9903,9904..10695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10053..10072)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10866..11454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11355..11374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11608..11625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11742..11763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11778..11783,11784..11801,11802..11808)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11816..11832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11821..11843
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11840..11856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11840..11856
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11875..11895
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11877..11895
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcac gagactagcc tcgagtttac cactccctat cagtgataga gaaaagtgaa
     1981 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2041 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2101 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2161 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2221 gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc
     2281 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     2341 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga attcgagctc
     2401 ggtacccggg gatcctctag tcagctgacg cgtaccatgg atctgagcgg cctgaccctg
     2461 caggaattca gctccccgaa acgccctaag ctgctgcgtg aatattacga ttggctggtt
     2521 gataacagct ttaccccata tctggtggtg gatgccacat acctgggcgt gaacgtgccc
     2581 gtggagtatg tgaaagacgg tcagatcgtg ctgaatctgt ctgcaagtgc gaccggcaac
     2641 ctgcaactga caaatgattt tatccagttc aacgcccagt ttaagggcgt gtctcgtgaa
     2701 ctgtatatcc cgatgggtgc cgctctggcc atttacgctc gcgagaacgg cgatggtgtg
     2761 atgttcgaac cagaagaaat ctatgacgag ctgaatattg gtcaattgtc tagaggtggc
     2821 gggggcagtg ggggaggggg aagtggaggc ggcggttctg gcgggggagg ctccggkggs
     2881 ggggggrgsg ggggkgggag taagcttgtg agcaagggcg aggaggataa catggccatc
     2941 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3001 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3061 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3121 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     3181 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3241 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3301 aacttcccct ccgacggtcc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3361 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     3421 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     3481 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     3541 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     3601 gagctgtaca aggctagcgg aggcggtggc tcaggcggag gagggtcagg agggggaggc
     3661 agtggcgggg gcggctctgg aggagggggt tctggtggtg gtagctacgt aatgagtctt
     3721 caaatgattg ttgagaatgt aaaattggct cgtgaatatg cactgctggg aaactatgac
     3781 tctgcaatgg tctactatca gggagttctt gaccaaatga acaagtacct gtactcagtc
     3841 aaagatacac acctccgtca gaaatggcaa caggtttggc aggaaataaa tgtggaagct
     3901 aagcaagtta aggatatcat gaaaacactc gagagcttca aactagacat cacttctttg
     3961 caagctgcac aacatgaatt tccagctgct gaaggagaag tctggtcctt gcctgtacct
     4021 gttgagcgaa gacccttacc aggacctagg aaacgccagt cttctcagca cagtgaccct
     4081 aagccacaca gtaaccggcc aagcacagtc gtcagagctc atcggccgtc tccacagaat
     4141 cttcacaatg acagagggaa agctgttcgt agtcgtgaaa agaaagaaca gagtaaggga
     4201 agagaggaaa agaacaaatt acctgctgca gttacagaac cagaggcaaa taagtttgat
     4261 ggcactgggt atgacaagga cttagtagaa gctttggaaa gagatatcat ttctcagaat
     4321 cccaacgttc ggtggtatga tattgctgat ttagtagaag ccaaaaagtt gcttcaggaa
     4381 gcagtggtgt taccaatgtg gatgccagaa ttctttaagg gcattaggag accatggaaa
     4441 ggtgtgctga tggttggccc acctggcact ggaaagaccc ttctagctaa agcagttgcc
     4501 acggaatgca agacaacgtt cttcaatgtc tcttcatcaa ctctgacgtc caaatacaga
     4561 ggagagtctg agaagcttgt ccgtctcctg tttgaaatgg ctcggtttta ctccccagct
     4621 accatattta ttgatgagat agattccatt tgcagccgcc gaggaacttc tgaagagcat
     4681 gaagccagcc gaaggatgaa agctgagctg ctggttcaaa tggacggtgt tggaggtgcc
     4741 tcagagaacg atgacccttc caaaatggtt atggttctgg cggctactaa tttcccatgg
     4801 gacatagatg aggctttaag gcgtcgtctg gagaaaagaa tctatattcc attaccatca
     4861 gcaaaaggca gggaagaact gttgagaata agtctgcgag agctggaatt ggctgatgat
     4921 gtcaaccttg cgagcatagc agaaaacatg gaaggctact cgggtgcaga cattaccaat
     4981 gtatgcaggg acgcatcgtt gatggccatg agacggcgta ttgagggtct aaccccagaa
     5041 gaaatccgga acctttcaag agaagcaatg cacatgccta ccaccatgga ggattttgaa
     5101 atggctttaa agaagatttc taaatcagta tcagctgcag atattgaaag atacgagaaa
     5161 tggatagttg agtttggatc atgctagacc ggtacgcgtg ctagcgcggc cgcatcgata
     5221 agcttgtcga cgatatcgaa ttccacgggg ttggggttgc gccttttcca aggcagccct
     5281 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     5341 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     5401 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     5461 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     5521 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     5581 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     5641 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     5701 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     5761 gatccgttta tctgcagaat tcgcccttga cgtcatgtct agactggaca agagcaaagt
     5821 cataaacggc gctctggaat tactcaatgg agtcggtatc gaaggcctga cgacaaggaa
     5881 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     5941 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc
     6001 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc
     6061 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     6121 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     6181 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca
     6241 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     6301 tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg
     6361 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc
     6421 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     6481 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta
     6541 aggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     6601 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     6661 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     6721 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     6781 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     6841 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     6901 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6961 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     7021 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     7081 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca
     7141 aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7201 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7261 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7321 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7381 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     7441 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7501 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7561 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7621 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7681 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7741 aagcccggtg cctagctgca gcacgtgtct agaaacgctg gtcgacaatc aacctctgga
     7801 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7861 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7921 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7981 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8041 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8101 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8161 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     8221 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8281 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8341 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta
     8401 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     8461 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     8521 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8581 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8641 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8701 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8761 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8881 gtatcttatc atgtctggct cyrscyatcc cgcccctaac tccgcccagt tccgcccatt
     8941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     9061 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     9121 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9181 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9241 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9301 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9361 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9421 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9481 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9541 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9601 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9661 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     9721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9901 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9961 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10021 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    10081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10141 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10201 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10261 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10381 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10681 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10741 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10801 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10981 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    11041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    11101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    11161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    11221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    11281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    11341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    11401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11521 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11581 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11641 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    11701 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    11761 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    11821 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
    11881 aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
