LOCUS       sequence-373746-        2224 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild-type myocilin with a C-terminal enhanced Gaussia 
            luciferase and FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Nakahara E, Hulleman JD
  TITLE     A Simple Secretion Assay for Assessing New and Existing Myocilin 
            Variants.
  JOURNAL   Curr Eye Res. 2022 Jun;47(6):918-922. doi: 
            10.1080/02713683.2022.2047205. Epub 2022 Mar 25.
  PUBMED    35196929
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/02713683.2022.2047205"; journalName: "Curr Eye Res"; date: 
            "2022-06"; volume: "47"; issue: "6"; pages: "918-922"
COMMENT     Sequence Label: pcDNA3 WT MYOC eGLuc2 FLAG
FEATURES             Location/Qualifiers
     source          1..2224
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2198..2221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagcttggta ccgagctcgg atccagagct ttccagagga agcctcacca
      121 agcctctgca atgaggttct tctgtgcacg ttgctgcagc tttgggcctg agatgccagc
      181 tgtccagctg ctgcttctgg cctgcctggt gtgggatgtg ggggccagga cagctcagct
      241 caggaaggcc aatgaccaga gtggccgatg ccagtatacc ttcagtgtgg ccagtcccaa
      301 tgaatccagc tgcccagagc agagccaggc catgtcagtc atccataact tacagagaga
      361 cagcagcacc caacgcttag acctggaggc caccaaagct cgactcagct ccctggagag
      421 cctcctccac caattgacct tggaccaggc tgccaggccc caggagaccc aggaggggct
      481 gcagagggag ctgggcaccc tgaggcggga gcgggaccag ctggaaaccc aaaccagaga
      541 gttggagact gcctacagca acctcctccg agacaagtca gttctggagg aagagaagaa
      601 gcgactaagg caagaaaatg agaatctggc caggaggttg gaaagcagca gccaggaggt
      661 agcaaggctg agaaggggcc agtgtcccca gacccgagac actgctcggg ctgtgccacc
      721 aggctccaga gaagtttcta cgtggaattt ggacactttg gccttccagg aactgaagtc
      781 cgagctaact gaagttcctg cttcccgaat tttgaaggag agcccatctg gctatctcag
      841 gagtggagag ggagacaccg gatgtggaga actagtttgg gtaggagagc ctctcacgct
      901 gagaacagca gaaacaatta ctggcaagta tggtgtgtgg atgcgagacc ccaagcccac
      961 ctacccctac acccaggaga ccacgtggag aatcgacaca gttggcacgg atgtccgcca
     1021 ggtttttgag tatgacctca tcagccagtt tatgcagggc tacccttcta aggttcacat
     1081 actgcctagg ccactggaaa gcacgggtgc tgtggtgtac tcggggagcc tctatttcca
     1141 gggcgctgag tccagaactg tcataagata tgagctgaat accgagacag tgaaggctga
     1201 gaaggaaatc cctggagctg gctaccacgg acagttcccg tattcttggg gtggctacac
     1261 ggacattgac ttggctgtgg atgaagcagg cctctgggtc atttacagca ccgatgaggc
     1321 caaaggtgcc attgtcctct ccaaactgaa cccagagaat ctggaactcg aacaaacctg
     1381 ggagacaaac atccgtaagc agtcagtcgc caatgccttc atcatctgtg gcaccttgta
     1441 caccgtcagc agctacacct cagcagatgc taccgtcaac tttgcttatg acacaggcac
     1501 aggtatcagc aagaccctga ccatcccatt caagaaccgc tataagtaca gcagcatgat
     1561 tgactacaac cccctggaga agaagctctt tgcctgggac aacttgaaca tggtcactta
     1621 tgacatcaag ctctccaaga tgatgttcga aggctccgct ggctccgctg ctggttctgg
     1681 cgaatttgag gccaagccca ccgagaacaa cgaagacttc aacatcgtgg ccgtggccag
     1741 caacttcgcg accacggatc tcgatgctga ccgcgggaag ttgcccggca agaagctgcc
     1801 gctggaggtg ctcaaagaga tagaagccaa tgcccggaaa gctggctgca ccaggggctg
     1861 tctgatctgc ctgtcccaca tcaagtgcac gcccaagatg aagaagttca tcccaggacg
     1921 ctgccacacc tacgaaggcg acaaagagtc cgcacagggc ggcataggcg aggcgatcgt
     1981 cgacattcct gagattcctg ggttcaagga cttggagccc atagagcagt tcatcgcaca
     2041 ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa gggcttgcca acgtgcagtg
     2101 ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg acctttgcca gcaagatcca
     2161 gggccaggtg gacaagatca agggggccgg tggtgacgat tacaaggacg acgatgacaa
     2221 gtga
//
