LOCUS       sequence-373763-        3342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for full-site selection assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Wang JY, Hoel CM, Al-Shayeb B, Banfield JF, Brohawn SG, Doudna JA
  TITLE     Structural coordination between active sites of a CRISPR reverse 
            transcriptase-integrase complex.
  JOURNAL   Nat Commun. 2021 May 6;12(1):2571. doi: 10.1038/s41467-021-22900-y.
  PUBMED    33958590
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-6"; volume: "12(1):2571. doi"; pages: " 
            10.1038/s41467-021-22900-y"
COMMENT     Sequence Label: pCRISPR_full-site
FEATURES             Location/Qualifiers
     source          1..3342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             24..680
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="EKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLK
                     TVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSL
                     WSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMD
                     NFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(87..106)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      701..795
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     892..914
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(952..970)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1038..1142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1143..1211,1212..2003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1361..1380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2174..2762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2663..2682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2916..2933
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3075..3165
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aaagccgaaa ggctgttaag acagagaaaa aaatcactgg atataccacc gttgatatat
       61 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      121 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      181 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
      241 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      301 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      361 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      421 tccctaaagg gtttattgag aatatgtttt tcgtgtcagc caatccctgg gtgagtttca
      481 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      541 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      601 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
      661 agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt gatagatcca
      721 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
      781 tttattggtg agaatcctta agccagcccc gacacccgcc aacacccgct gacgcgccct
      841 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      901 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      961 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1381 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1681 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2281 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2941 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3001 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatc ctctagagga
     3061 tccaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
     3121 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaaatc aacattaaaa
     3181 tatggtgcgg gaactatgaa aaacaaacaa aaacaaccat ccatacctca ccaaaatacc
     3241 cccggtaacc catcaaacac accccaaatc attctggtgc ggaaaatgca ttgcaactca
     3301 ttgaataagc agatagaatt tcaaccggag ttctagaaca cc
//
