LOCUS       sequence-373779-       16276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant rabies virus expressing histone-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16276)
  AUTHORS   Yao S, Wang Q, Hirokawa KE, Ouellette B, Ahmed R, Bomben J, Brouner 
            K, Casal L, Caldejon S, Cho A, Dotson NI, Daigle TL, Egdorf T, 
            Enstrom R, Gary A, Gelfand E, Gorham M, Griffin F, Gu H, Hancock N, 
            Howard R, Kuan L, Lambert S, Lee EK, Luviano J, Mace K, Maxwell M, 
            Mortrud MT, Naeemi M, Nayan C, Ngo NK, Nguyen T, North K, Ransford 
            S, Ruiz A, Seid S, Swapp J, Taormina MJ, Wakeman W, Zhou T, Nicovich
            PR, Williford A, Potekhina L, McGraw M, Ng L, Groblewski PA, Tasic 
            B, Mihalas S, Harris JA, Cetin A, Zeng H
  TITLE     A whole-brain monosynaptic input connectome to neuron classes in 
            mouse visual cortex.
  JOURNAL   Nat Neurosci. 2023 Feb;26(2):350-364. doi: 
            10.1038/s41593-022-01219-x. Epub 2022 Dec 22.
  PUBMED    36550293
REFERENCE   2  (bases 1 to 16276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-022-01219-x"; journalName: "Nat Neurosci"; date: 
            "2023-02"; volume: "26"; issue: "2"; pages: "350-364"
COMMENT     Sequence Label: AiP5002 RabV-SADdeltaG-histone-EGFP
FEATURES             Location/Qualifiers
     source          1..16276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        115..162
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             181..555
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    685..806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(722..741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     776..795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(855..871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(855..871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(868..890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    879..895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(903..909,910..927,928..933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    948..969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1086..1103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1257..1845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1337..1356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2016..2807,2808..2876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2639..2658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2877..2981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3056..3075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3247..3626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3627..3830
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3781..3801
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3875..3894
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3875..3893
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7344..7721
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      7734..7743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7740..7742,7743..7745,7746..8459)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7740..7742,7743..7745,7746..8456)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7785..7806)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8046..8065)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8393..8414
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10187..10198
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(15296..15313)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15302..15526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      15572..16000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15659..15678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15869..15890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(16009..16029)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(16014..16276,1..67)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(16194..16276,1..53)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     16256..16275
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
       61 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
      121 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
      181 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
      241 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
      301 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
      361 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
      421 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
      481 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
      541 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
      601 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
      661 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
      721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      781 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
      841 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      901 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      961 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1021 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1981 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2041 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2101 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2161 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2221 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2281 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2341 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2401 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2461 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2521 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2581 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2641 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2701 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2761 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2821 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2881 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2941 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3001 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     3061 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     3121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     3181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     3241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     3301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
     3841 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
     3901 aagctggcta gattaagcgt ctgatgagtc cgtgaggacg aaacccggag tcccgggtca
     3961 cgcttaacaa ccagatcaaa gaaaaaacag acattgtcaa ttgcaaagca aaaatgtaac
     4021 acccctacaa tggatgccga caagattgta ttcaaagtca ataatcaggt ggtctctttg
     4081 aagcctgaga ttatcgtgga tcaatatgag tacaagtacc ctgccatcaa agatttgaaa
     4141 aagccctgta taaccctagg aaaggctccc gatttaaata aagcatacaa gtcagttttg
     4201 tcaggcatga gcgccgccaa acttaatcct gacgatgtat gttcctattt ggcagcggca
     4261 atgcagtttt ttgaggggac atgtccggaa gactggacca gctatggaat tgtgattgca
     4321 cgaaaaggag ataagatcac cccaggttct ctggtggaga taaaacgtac tgatgtagaa
     4381 gggaattggg ctctgacagg aggcatggaa ctgacaagag accccactgt ccctgagcat
     4441 gcgtccttag tcggtcttct cttgagtctg tataggttga gcaaaatatc cgggcaaaac
     4501 actggtaact ataagacaaa cattgcagac aggatagagc agatttttga gacagcccct
     4561 tttgttaaaa tcgtggaaca ccatactcta atgacaactc acaaaatgtg tgctaattgg
     4621 agtactatac caaacttcag atttttggcc ggaacctatg acatgttttt ctcccggatt
     4681 gagcatctat attcagcaat cagagtgggc acagttgtca ctgcttatga agactgttca
     4741 ggactggtat catttactgg gttcataaaa caaatcaatc tcaccgctag agaggcaata
     4801 ctatatttct tccacaagaa ctttgaggaa gagataagaa gaatgtttga gccagggcag
     4861 gagacagctg ttcctcactc ttatttcatc cacttccgtt cactaggctt gagtgggaaa
     4921 tctccttatt catcaaatgc tgttggtcac gtgttcaatc tcattcactt tgtaggatgc
     4981 tatatgggtc aagtcagatc cctaaatgca acggttattg ctgcatgtgc tcctcatgaa
     5041 atgtctgttc tagggggcta tctgggagag gaattcttcg ggaaagggac atttgaaaga
     5101 agattcttca gagatgagaa agaacttcaa gaatacgagg cggctgaact gacaaagact
     5161 gacgtagcac tggcagatga tggaactgtc aactctgacg acgaggacta cttttcaggt
     5221 gaaaccagaa gtccggaggc tgtttatact cgaatcatga tgaatggagg tcgactaaag
     5281 agatctcaca tacggagata tgtctcagtc agttccaatc atcaagcccg tccaaactca
     5341 ttcgccgagt ttctaaacaa gacatattcg agtgactcat aagaagttga ataacaaaat
     5401 gccggaaatc tacggattgt gtatatccat catgaaaaaa actaacaccc ctcctttcga
     5461 accatcccaa acatgagcaa gatctttgtc aatcctagtg ctattagagc cggtctggcc
     5521 gatcttgaga tggctgaaga aactgttgat ctgatcaata gaaatatcga agacaatcag
     5581 gctcatctcc aaggggaacc catagaggtg gacaatctcc ctgaggatat ggggcgactt
     5641 cacctggatg atggaaaatc gcccaaccat ggtgagatag ccaaggtggg agaaggcaag
     5701 tatcgagagg actttcagat ggatgaagga gaggatccta gcttcctgtt ccagtcatac
     5761 ctggaaaatg ttggagtcca aatagtcaga caaatgaggt caggagagag atttctcaag
     5821 atatggtcac agaccgtaga agagattata tcctatgtcg cggtcaactt tcccaaccct
     5881 ccaggaaagt cttcagagga taaatcaacc cagactactg gccgagagct caagaaggag
     5941 acaacaccca ctccttctca gagagaaagc caatcatcga aagccaggat ggcggctcaa
     6001 attgcttctg gccctccagc ccttgaatgg tcggctacca atgaagagga tgatctatca
     6061 gtggaggctg agatcgctca ccagattgca gaaagtttct ccaaaaaata taagtttccc
     6121 tctcgatcct cagggatact cttgtataat tttgagcaat tgaaaatgaa ccttgatgat
     6181 atagttaaag aggcaaaaaa tgtaccaggt gtgacccgtt tagcccatga cgggtccaaa
     6241 ctccccctaa gatgtgtact gggatgggtc gctttggcca actctaagaa attccagttg
     6301 ttagtcgaat ccgacaagct gagtaaaatc atgcaagatg acttgaatcg ctatacatct
     6361 tgctaaccga acctctcccc tcagtccctc tagacaataa aatccgagat gtcccaaagt
     6421 caacatgaaa aaaacaggca acaccactga taaatcgatg aacctcctac gtaagatagt
     6481 gaaaaaccgc agggacgagg acactcaaaa atcctctccc gcgtcagccc ctctggatga
     6541 cgatgacttg tggcttccac cccctgaata cgtcccgctg aaagaactta caggcaagaa
     6601 gaacatgagg aacttttgta tcaacggaag ggttaaagtg tgtagcccga atggttactc
     6661 gttcaggatc ctgcggcaca ttctgaaatc attcgacgag atatattctg ggaatcatag
     6721 gatgatcggg ttagtcaaag tggttattgg actggctttg tcaggatctc cagtccctga
     6781 gggcctgaac tgggtataca aattgaggag aacctttatc ttccagtggg ctgattccag
     6841 gggccctctt gaaggggagg agttggaata ctctcaggag atcacttggg atgatgatac
     6901 tgagttcgtc ggattgcaaa taagagtgat tgcaaaacag tgtcatatcc agggcagagt
     6961 ctggtgtatc aacatgaacc cgagagcatg tcaactatgg tctgacatgt ctcttcagac
     7021 acaaaggtcc gaagaggaca aagattcctc tctgcttcta gaataacgta cgtcagatta
     7081 tatcccgcaa atttatcact tgtttacctc tggaggagag aacatatggg ctcaactcca
     7141 acccttggga gcaatataac aaaaaacatg ttatggtgcc attaaaccgc tgcatttcat
     7201 caaagtcaag ttgattacct ttacattttg atcctcttgg atgtgaaaaa aactattaac
     7261 atccctcaaa ggacctgcag gtacgcggcc gctacgcccg ggctacgcta gcatgaaaaa
     7321 aactaacacc cctccttaat taaatgccag agccagcgaa gtctgctccc gccccgaaaa
     7381 agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag cgcaagcgca
     7441 gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc caccctgaca
     7501 ccggcatttc gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac attttcgagc
     7561 gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc atcacctcca
     7621 gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag cacgccgtgt
     7681 ccgagggtac taaggccatc accaagtaca ccagcgctaa ggatccaccg gtcgccacca
     7741 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     7801 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     7861 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     7921 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     7981 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     8041 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     8101 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     8161 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     8221 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     8281 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     8341 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     8401 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac
     8461 cgcggtagct tttcagtcga gaaaaaaaca ttagatcaga agaacaactg gcaacacttc
     8521 tcaacctgag acttacttca agatgctcga tcctggagag gtctatgatg accctattga
     8581 cccaatcgag ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa
     8641 ctctgactac aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg
     8701 gttaaaaaca gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt
     8761 cagagttttg aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat
     8821 ggctgcacag tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag
     8881 ccggagatgt ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct
     8941 gttgaatctc acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg
     9001 ccttgagagg gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc
     9061 ttacttgttc ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa
     9121 gaccatccta gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa
     9181 gttcaaagac caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag
     9241 ttccaattgt ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg
     9301 cttcaactcc ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat
     9361 atctcaacta tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc
     9421 cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc
     9481 agaaaagttt aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa
     9541 ggtaagtcaa cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga
     9601 tcaattcgac aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca
     9661 cccatatata gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa
     9721 aatgatagat aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag
     9781 atggggtttt gataagtact ccaagtggta tctggattca agattcctag cccgagacca
     9841 ccccttgact ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt
     9901 gggggataca tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga
     9961 tccgtcagaa atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg
    10021 gctgtcagaa aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc
    10081 taagccgcct gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga
    10141 tgaagacttg ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt
    10201 ctttgctcta atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc
    10261 caactacatc ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt
    10321 taaaaagctg atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata
    10381 tgcatttcac ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga
    10441 tgtattttct gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga
    10501 gttttttcaa aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga
    10561 ggatcaaata tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg
    10621 cgggctagaa ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag
    10681 agaatctcaa atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt
    10741 atgtccgaca tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga
    10801 gagaatatca aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct
    10861 agggctgatc atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa
    10921 aacccctttg tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc
    10981 ttgcgtctct aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa
    11041 tgcgctaaca gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct
    11101 catgtcagta caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt
    11161 ttacaagatt ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta
    11221 tctagatcct tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca
    11281 gttctcagac cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca
    11341 agagtcctgg attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac
    11401 actcgagagc ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc
    11461 cagtcctacc attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa
    11521 ggtggaaaat tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt
    11581 tatactcttc ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag
    11641 ttcgtctttt ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat
    11701 aagaaggcag tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga
    11761 gatccacggg attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg
    11821 ctcttcagag agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac
    11881 gacagttcct cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac
    11941 ttgtggagca acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga
    12001 tcagtcattt ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc
    12061 gacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct
    12121 atcgttaaaa gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct
    12181 aattaggaac attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt
    12241 caaaaggacg gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta
    12301 ttcttctgtc tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga
    12361 tttgacccaa gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca
    12421 gacatggaca tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg
    12481 gcacctccga tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca
    12541 gatcttcgag tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc
    12601 tcacttccag aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg
    12661 tagagaaaag tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc
    12721 aattcacgac tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt
    12781 ttcccctaga gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg
    12841 cttcttgaca agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt
    12901 aatctcatat attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga
    12961 accgtctctt agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac
    13021 cactatgaaa gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga
    13081 gcgagagata atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag
    13141 aagtgccaaa atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag
    13201 ggttgagaga aacctatcta agagtatgag agataacctg cgacaattga gttctttgat
    13261 gaggcaggtg ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg
    13321 actgctaaaa gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc
    13381 tagaaccatg actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc
    13441 agaatgggtc tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt
    13501 ctcggagctt gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt
    13561 gagagtggtt cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct
    13621 caatgttttc ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc
    13681 agtcctcaac atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga
    13741 cctgatggct tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga
    13801 tatcgtctcc agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa
    13861 cttggcaacc tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct
    13921 cattatttgc gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat
    13981 gtccgatttt gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac
    14041 tatgctagta aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt
    14101 cacagggttt atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc
    14161 caaacgaggg aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat
    14221 gagccttgtg ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt
    14281 gaactatcag gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga
    14341 gatgatcata actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga
    14401 tgatcttgag ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat
    14461 agttttctcc aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc
    14521 accgtctgat cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct
    14581 atctactgct ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc
    14641 tataacttat tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg
    14701 gtccaacgac acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc
    14761 tcactggatc aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa
    14821 ggatctatcc agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga
    14881 ggatatcaga tctagatcat ccctactaga ctacagttgc ctgtgaaccg gatactcctg
    14941 gaagcctgcc catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa gatcctaaat
    15001 ctgaaccttt ggttgtttga ttgtttttct catttttgtt gtttatttgt taagcgtggg
    15061 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgcacgt
    15121 ccactcggat ggctaaggga gaagggcgaa ttccagcaca ctggcggccg ttactagtgg
    15181 atccgagctc ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat
    15241 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
    15301 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
    15361 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
    15421 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
    15481 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
    15541 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
    15601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    15661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    15721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    15781 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    15841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    15901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    15961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
    16021 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    16081 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    16141 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    16201 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    16261 atgcagaggc cgaggc
//
