LOCUS       sequence-373781-       11691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 
            3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be 
            integrated with pBac..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Kandul NP, Liu J, Buchman A, Shriner IC, Corder RM, Warsinger-Pepe 
            N, Yang T, Yadav AK, Scott MJ, Marshall JM, Akbari OS
  TITLE     Precision Guided Sterile Males Suppress Populations of an Invasive 
            Crop Pest
  JOURNAL   GEN Biotechnology (2022) 1:4, 372-385
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GEN 
            Biotechnology (2022) 1:4, 372-385"
COMMENT     Sequence Label: 1161F
FEATURES             Location/Qualifiers
     source          1..11691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_RNA        complement(1630..1705)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1726..2145)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(2421..2496)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(2517..2936)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(3575..3650)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3671..4090)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     CDS             complement(join(4766..5479,5480..5482))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(4769..5479,5480..5482))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4811..4832)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5160..5179
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5419..5440
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(5499..6090)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     protein_bind    6586..6971
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     polyA_signal    7230..7351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7267..7286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7321..7340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7356..7775
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        7796..7871
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8146..8565
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        8586..8661
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8923..8992
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   9462..9496
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9503..9521)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9542..9558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9542..9558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9555..9577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9566..9582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9590..9596,9597..9614,9615..9620))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9635..9656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9773..9790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9944..10532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10024..10043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10703..11494,11495..11563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11326..11345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11564..11668)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cgcgtatgca tacgcattaa gcgaacatta aaaagatgtg aaaacataac
     1441 tattatgtct aaataaacac acgtcagatg tatgtacgtc aacggaaaac cattgtctat
     1501 atattacaat tactaaatac ataccaaatt gaatacatat tgatgaaaaa taataaatac
     1561 tggcgaaagc aaaaaaacga aacattttta ttttattgaa caactctcag gctccaggta
     1621 ggcaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
     1681 aacttgctat ttctagctct aaaacccgat ctggttaccg cattgcgacg ttaaattgaa
     1741 aataggtcta tatatacgaa ctgagtctgg aaaaagaagt tgagaattat aaaaagtagt
     1801 gagcactggc gccctctctg cttggcgagc taaccttttc gcctcttggc tgagtaggtg
     1861 gcgtttcatt ctactctgta aaattaatgt agaattgaaa cactatggtc aaaaaatact
     1921 taggggcata agttataaaa cgtatgaaat gaatttttat caacctgggc tattcaaaaa
     1981 ttttcgaatt attttatgta ttttttttaa tcgtttttca ttataggtta aaatacactt
     2041 taaaaggaat tctttcctgt aaaataaata taaataaata tgctttattg acagaaaatt
     2101 tgatgttttt gtatttgagt aggagcaatc acaggtgagc aaaaagtgta tcactgacag
     2161 ctcgactctc tacgatatct aagctgatgc atacgcatta agcgaacatt aaaaagatgt
     2221 gaaaacataa ctattatgtc taaataaaca cacgtcagat gtatgtacgt caacggaaaa
     2281 ccattgtcta tatattacaa ttactaaata cataccaaat tgaatacata ttgatgaaaa
     2341 ataataaata ctggcgaaag caaaaaaacg aaacattttt attttattga acaactctca
     2401 ggctccaggt aggcaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
     2461 agccttattt taacttgcta tttctagctc taaaacttcc ggagccggta ccgccacgac
     2521 gttaaattga aaataggtct atatatacga actgagtctg gaaaaagaag ttgagaatta
     2581 taaaaagtag tgagcactgg cgccctctct gcttggcgag ctaacctttt cgcctcttgg
     2641 ctgagtaggt ggcgtttcat tctactctgt aaaattaatg tagaattgaa acactatggt
     2701 caaaaaatac ttaggggcat aagttataaa acgtatgaaa tgaattttta tcaacctggg
     2761 ctattcaaaa attttcgaat tattttatgt atttttttta atcgtttttc attataggtt
     2821 aaaatacact ttaaaaggaa ttctttcctg taaaataaat ataaataaat atgctttatt
     2881 gacagaaaat ttgatgtttt tgtatttgag taggagcaat cacaggtgag caaaaaggcg
     2941 cgcctcctga ccgtccatcg caataaaatg agccattatg agatcgaaag ggtctacgaa
     3001 aatccgttcg gaaaaaatca gaaaatcatc aaagccgaat ataattaaaa tgtattacta
     3061 gctaaagaaa tcatcactaa tatagaatgt agaatgaacc catgtatatt agatactaat
     3121 tgtatcgtaa gactttcaaa agtctacaag acattaaatg acaagttgac tttaaatttc
     3181 aaataaataa tttatttttt ctataagcaa taacattttt gctaaattaa gacttggtaa
     3241 ttaggtaata ctattgttgt tctatggaat attcgatcga aacattctta tcagtctcaa
     3301 aaacttaaaa caaacttata atataaccca tatgcctagg atgcatacgc attaagcgaa
     3361 cattaaaaag atgtgaaaac ataactatta tgtctaaata aacacacgtc agatgtatgt
     3421 acgtcaacgg aaaaccattg tctatatatt acaattacta aatacatacc aaattgaata
     3481 catattgatg aaaaataata aatactggcg aaagcaaaaa aacgaaacat ttttatttta
     3541 ttgaacaact ctcaggctcc aggtaggcaa aaaagcaccg actcggtgcc actttttcaa
     3601 gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac acatctttag
     3661 accccgaggc cgacgttaaa ttgaaaatag gtctatatat acgaactgag tctggaaaaa
     3721 gaagttgaga attataaaaa gtagtgagca ctggcgccct ctctgcttgg cgagctaacc
     3781 ttttcgcctc ttggctgagt aggtggcgtt tcattctact ctgtaaaatt aatgtagaat
     3841 tgaaacacta tggtcaaaaa atacttaggg gcataagtta taaaacgtat gaaatgaatt
     3901 tttatcaacc tgggctattc aaaaattttc gaattatttt atgtattttt tttaatcgtt
     3961 tttcattata ggttaaaata cactttaaaa ggaattcttt cctgtaaaat aaatataaat
     4021 aaatatgctt tattgacaga aaatttgatg tttttgtatt tgagtaggag caatcacagg
     4081 tgagcaaaaa cctaggtcga atcgctatcc aagccagctc cattgtcggc atcgtgctca
     4141 ttctattgac gctggcagat ttggttttgg cgctatggga cccgttcggt tacaacaaca
     4201 tgtttccgcg cgagtttccc gacgacatgt cgcgcacgtt cctgactgcg tactttgaga
     4261 gtttcgacaa caccacgtcc agagaaatca tagagtttat gcccgagttc ttttcggaaa
     4321 tggtcgaaac ggacgatgac gccacgtttg aatctctatt tcatttatta gattatgtgg
     4381 catctttaga agttaattcc gacggccaaa tgttaaactt ggaggagggt gatgaaattg
     4441 aggattttga cgaatctact ttggtggggc aagcgttagc cactagctcg ctatacactc
     4501 gcatggagtt tatgcagtac acgtttaggc aaaacacact attgtctatg aacaaagaaa
     4561 acaacaattt taatcaaata atcatgggtt tatttgcaac aaacacaatt gtggcgttta
     4621 cagcatttgt tatacacaca gaactcatat tttttatatt tttcgtaatc ttcctaatga
     4681 tcacatttta ttacataatc aaagaatcgt acgaatatta taaaacaatt gatttgttat
     4741 tttaaaaacg attcatggat ccctattact tgtacagctc gtccatgccg agagtgatcc
     4801 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     4861 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     4921 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     4981 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     5041 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     5101 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     5161 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     5221 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     5281 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     5341 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     5401 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     5461 acagctcctc gcccttgctc attttgttaa ttaagtcact tggttgttca cgatcttgtc
     5521 gccgccagtg tcaacttgca actgaaacaa tatccaacat gaacgtcaat ttatactgcc
     5581 ctaatggcga acacgataac aatatttctt ttattatgcc ctctaaaacc aacgcggtta
     5641 tcgtttattt attcaaatta gatatagaac atccgccgac atacaatgtt aatgcaaaaa
     5701 cgcgtttggt gagcggatac gaaaacagtc ggccgataaa cattaatctg aggtcgataa
     5761 caccgtcctt gaacggaaca cgaggagcgt acgtgatcag ctgcattcgc gcgccgcgcc
     5821 tttatcgaga tttatttgca tacaacaagt acactgcgcc gttgggattt gtggtaacgc
     5881 gcacacatgc agagctgcaa gtgtggcaca ttttgtctgt gcgcaaaacc tttgaagcca
     5941 aaagtacgag gtccgttacg ggcatgctag cgcacacgga caatggaccc gacaaattct
     6001 acgccaagga tttaatgata atgtcgggca acgtatccgt tcattttatc aataacctac
     6061 aaaaatgtcg cgcgcatcac aaagacatcg acgcgcgtag aattctaccc gtaaagcgag
     6121 tttagttatg agccatgtgc aaaacatgac atcagctttt atttttataa caaatgacat
     6181 catttcttga ttgtgtttta cacgtagaat tctactcgta aagcgagttc agttttgaaa
     6241 aacaaatgac atcatctttt tgattgtgct ttacaagtag aattctaccc gtaaatcaag
     6301 ttcggttttg aaaaacaaat gagtcatatt gtatgatatc atattgcaaa acaaatgact
     6361 catcaatcga tcgtgcgtta cacgtagaat tctactcgta aagcgagttt atgagccgtg
     6421 tgcaaaacat gacatcakmt yatrwykcaw amywratkrc kcatcaatcg atcgtgcgtt
     6481 acacgtagaa ttctactcgt aaagcgagtt tatgagccgt gtgcaaaaca tgacatcagc
     6541 ttatgactca tacttgattg tgttttacgc gactagtagg tacctcacgt aataagtgtg
     6601 cgttgaattt attcgcaaaa acattgcata ttttcggcaa agtaaaattt tgttgcatac
     6661 cttatcaaaa aataagtgct gcatactttt tagagaaacc aaataatttt ttattgcata
     6721 cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg catactttga
     6781 cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac ccgtttttaa
     6841 taaaatacat tgcataccct cttttaataa aaaatattgc atacgttgac gaaacaaatt
     6901 ttcgttgcat acccaataaa agattattat attgcatacc ttttcttgcc ataccattta
     6961 gccgatcaat tccgcggcat actcggtggc ctccccacca ccaacttttt tgcactgcaa
     7021 aaaaacacgc ttttgcacgc gggcccatac atagtacaaa ctctacgttt cgtagactat
     7081 tttacataaa tagtctacac cgttgtatac gctccaaata cactaccaca cattgaacct
     7141 ttttgcagtg caaaaaagta cgtgtcggca gtcacgtagg ccggccttat cgggtcgcgt
     7201 cctgtcacgt acgaatcaca ttatcggtta acttgtttat tgcagcttat aatggttaca
     7261 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7321 gtggtttgtc caaactcatc aatgtatctt aaagcttttt gctcacctgt gattgctcct
     7381 actcaaatac aaaaacatca aattttctgt caataaagca tatttattta tatttatttt
     7441 acaggaaaga attcctttta aagtgtattt taacctataa tgaaaaacga ttaaaaaaaa
     7501 tacataaaat aattcgaaaa tttttgaata gcccaggttg ataaaaattc atttcatacg
     7561 ttttataact tatgccccta agtatttttt gaccatagtg tttcaattct acattaattt
     7621 tacagagtag aatgaaacgc cacctactca gccaagaggc gaaaaggtta gctcgccaag
     7681 cagagagggc gccagtgctc actacttttt ataattctca acttcttttt ccagactcag
     7741 ttcgtatata tagacctatt ttcaatttaa cgtcgggcgg cagcggcggg aatgggtttt
     7801 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     7861 cgagtcggtg cttttttgcc tacctggagc ctgagagttg ttcaataaaa taaaaatgtt
     7921 tcgttttttt gctttcgcca gtatttatta tttttcatca atatgtattc aatttggtat
     7981 gtatttagta attgtaatat atagacaatg gttttccgtt gacgtacata catctgacgt
     8041 gtgtttattt agacataata gttatgtttt cacatctttt taatgttcgc ttaatgcgta
     8101 tgcatctccg ttgtatgttg tagcgtctgt gaagcgtcct actccttttt gctcacctgt
     8161 gattgctcct actcaaatac aaaaacatca aattttctgt caataaagca tatttattta
     8221 tatttatttt acaggaaaga attcctttta aagtgtattt taacctataa tgaaaaacga
     8281 ttaaaaaaaa tacataaaat aattcgaaaa tttttgaata gcccaggttg ataaaaattc
     8341 atttcatacg ttttataact tatgccccta agtatttttt gaccatagtg tttcaattct
     8401 acattaattt tacagagtag aatgaaacgc cacctactca gccaagaggc gaaaaggtta
     8461 gctcgccaag cagagagggc gccagtgctc actacttttt ataattctca acttcttttt
     8521 ccagactcag ttcgtatata tagacctatt ttcaatttaa cgtcggattg tcaactactt
     8581 gccccgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     8641 aaagtggcac cgagtcggtg cttttttgcc tacctggagc ctgagagttg ttcaataaaa
     8701 taaaaatgtt tcgttttttt gctttcgcca gtatttatta tttttcatca atatgtattc
     8761 aatttggtat gtatttagta attgtaatat atagacaatg gttttccgtt gacgtacata
     8821 catctgacgt gtgtttattt agacataata gttatgtttt cacatctttt taatgttcgc
     8881 ttaatgcgta tgcataagct tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg
     8941 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat
     9001 catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa
     9061 gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc
     9121 gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat
     9181 gtcgacagat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     9241 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     9301 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     9361 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     9421 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     9481 gactatcttt ctagggttaa tccctttagt gagggttaat ttcgagcttg gcgtaatcat
     9541 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9601 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9661 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9721 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9781 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9841 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9901 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9961 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10021 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10081 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10141 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10201 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10261 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10321 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10381 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10441 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10501 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10561 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10621 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10681 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10741 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10801 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10861 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10921 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10981 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11041 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11101 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11161 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11221 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11281 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    11341 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    11401 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11461 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11521 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11581 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11641 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
