LOCUS       sequence-373787-         345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes SaCas9 and an sgRNA targeting mouse Kcna2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Yee JX, Rastani A, Soden ME
  TITLE     The potassium channel auxiliary subunit Kvbeta2 (Kcnab2) regulates 
            Kv1 channels and dopamine neuron firing.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):62-72. doi: 10.1152/jn.00194.2022.
            Epub 2022 Jun 15.
  PUBMED    35788155
REFERENCE   2  (bases 1 to 345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00194.2022"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "62-72"
COMMENT     Sequence Label: pAAV-FLEX-SaCas9-U6-sgKcna2
FEATURES             Location/Qualifiers
     source          1..345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        271..345
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg aagcaatgga gatgtttcgg gttttagtac tctggaaaca gaatctacta
      301 aaacaaggca aaatgccgtg tttatctcgt cacttgttgg cgaga
//
