LOCUS       sequence-373794-        7908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Liu B, Stone OJ, Pablo M, Herron JC, Nogueira AT, Dagliyan O, Grimm 
            JB, Lavis LD, Elston TC, Hahn KM
  TITLE     Biosensors based on peptide exposure show single molecule 
            conformations in live cells.
  JOURNAL   Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 
            10.1016/j.cell.2021.09.026. Epub 2021 Oct 11.
  PUBMED    34637702
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09.026"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5670-5685.e23"
COMMENT     Sequence Label: binder/tag-WT-FAK-insertionA
FEATURES             Location/Qualifiers
     source          1..7908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      704..713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             710..1417
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(855..873)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1123..1142
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4607..4633
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4637..4663
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4793..4914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4830..4849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4884..4903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4921..5376)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5058..5079)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5270..5289
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5403..5507
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5509..5866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5509..5700
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5532..5552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5717..5852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5901..6695
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5901..6695
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5955..5974)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6565..6584
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6883..6902)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6927..6974
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7303..7891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7792..7811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
       61 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
      721 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
      781 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      841 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      901 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
      961 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     1021 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     1081 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     1141 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     1201 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     1261 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     1321 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     1381 ccgccactcc accggcggca tggacgagct gtacaagtcc ggcatggcag ctgcttatct
     1441 tgacccaaac ttgaatcaca caccaagttc gagtactaag actcacctgg gtactggcat
     1501 ggaacggtcc cctggtgcaa tggaacgagc tgcaaatgat gaaaactacg tattaaaggt
     1561 ctttcatcat tttgaaagca gtagtgagcc aaccacctgg gccagtatta tcaggcatgg
     1621 agatgctact gatgtcaggg gcatcattca gaagatagtg gacagtcaca aagtaaagca
     1681 cgtggcctgc tatggatttc gcctcagtca cctgcgatcg gaggaggtgc actggctgca
     1741 cgtggacatg ggtgtctcca gtgtgaggga gaaatatgaa ctcgcccacc caccagagga
     1801 gtggaaatat gagttgagaa ttcgttactt gccaaaagga tttctaaacc agtttactga
     1861 agataagcca acattgaatt tcttctatca acaggtgaag agtgactaca tgcaagaaat
     1921 agctgatcaa gtagaccaag aaatagcttt gaagttgggt tgtttggaaa ttaggcgatc
     1981 ctattgggag atgaggggta atgccctaga gaagaagtcc aactatgaag tattagaaaa
     2041 agatgttggt ttaaagcgat tttttcctaa gagtttactg gattctgtca aggccaaaac
     2101 actaagaaag ctgatccagc aaacattcag gcagtttgcc aaccttaata gagaagaaag
     2161 catcctgaaa ttctttgaga ttctttctcc tgtgtacaga tttgacaaag agtgcttcaa
     2221 gtgtgccctt gggtcaagtt ggatcatttc tgtggaattg gcaatcggcc cagaagaagg
     2281 gatcagttac ctgacagaca aaggctgcaa tcccacacac ctagcagact ttaaccaagt
     2341 gcagaccatc cagtactcaa acagtgaaga caaagacagg aaaggaatgc tacaactcaa
     2401 aatcgctggt gctcctgagc ctctgaccgt gacggcacca tccctaacca ttgccgagaa
     2461 catggccgac ctgattgatg gatactgccg gctggtgaat ggagccacac aatctttcat
     2521 catcagaccc cagaaagaag gtgaacgggc gttgccatca ataccaaagt tggccaacag
     2581 tgaaaagcaa ggcatgcgga cacatgcagt ctctgtgtca gagacagatg actatgcaga
     2641 gatcatcgat gaggaagaca catacaccat gccctcgacc agggattatg agattcagag
     2701 agaaagaata gaacttggac gctgtattgg agaaggtcag tttggagatg tacatcaagg
     2761 cgtgtacctg agcccagaga atccagcttt ggctgttgca atcaaaacat gtaaaaactg
     2821 tacttcggac agcgtgagag agaagttcct tcaagaagcc ttaacaatgc gtcagtttga
     2881 ccatcctcac atcgtgaagc tgattggagt cattacagag aaccctgtct ggataatcat
     2941 ggagttgtgc acacttggag agctgaggtc atttttgcaa gtaagaaaat acagcttgga
     3001 cctggcatct ttgatattat atgcctatca gcttagtaca gcactcgcgt atctggagag
     3061 caaaagattt gttcacaggg acattgctgc tcggaatgtt ctggtgtctt caaatgattg
     3121 tgtaaaattg ggagactttg gattatctcg atatatggaa gacagtactt actataaagc
     3181 ttccaaagga aaattaccta ttaaatggat ggctccagag tcaatcaatt ttcgacgttt
     3241 tacctcagct agtgacgtgt ggatgtttgg tgtgtgtatg tgggagatac tgatgcatgg
     3301 cgtgaagcct tttcaaggag tgaagaacaa tgatgtgatc ggtcgaattg aaaatgggga
     3361 aagattacca atgcctccaa attgtcctcc caccctctac agccttatga cgaaatgttg
     3421 ggcctatgac cccagcaggc ggcccaggtt tactgaacta aaagctcagc tcagcacaat
     3481 cctggaggag gagaaggtgc agcaagaaga acggatgagg atggaatcca gaagacaggc
     3541 taccgtgtcc tgggactctg gagggtctga tgaagcacca cccaagccca gcagacctgg
     3601 ttatcctagc ccgagatcca gtgaaggatt ttatcccagc ccacagcaca tggtccagac
     3661 caatcactac caggtctctg gctacccggg ttcccatgga atcccagcca tggctggcag
     3721 catttaccaa ggtcaggcat ctcttctgga ccagacagaa ttgtggaatc acagacctca
     3781 ggagatgtcc atgtggcagc ccagtgtgga ggattcggca gctttggacc ttcgagggat
     3841 gggacaggtg cttccccctc acctgatgga agagcggcta atccgacagc agcaggaaat
     3901 ggaagaagac cagcgctggc tggagaagga agagcgattc ctgaaacctg atgtgaggct
     3961 ctctcgaggc agcatcgaca gggaagacgg gagttttcag ggtccgactg gaaaccaaca
     4021 catctatcag cctgtgggga agccagatcc tgcagctcca ccaaagaaac ctcctcgccc
     4081 tggagcacct ggccacctaa gcaacctgtc cagcatcagc agccctgcag acagctacaa
     4141 tgagggtgtc aagcttcagc cccaggaaat cagcccccct cccactgcca accttgaccg
     4201 gtccaatgac aaggtatatg aaaatgtgac aggcctagtg aaggctgtca tcgagatgtc
     4261 cagcaaaatc cagccagctc ctccagaaga gtacgtccct atggtgaagg aagttggcct
     4321 ggccctgcgg accttactag ccacggtgga tgagaccatt cctgctcttc cagccagcac
     4381 tcatcgagag atcgagatgg cacagaagct gctgaactcc gacttaggcg agctcatcag
     4441 caagatgaag ctggcgcagc agtacgtcat gaccagcctg cagcaggagt ataagaagca
     4501 gatgctgaca gctgctcacg ccctggctgt ggatgccaag aacctacttg atgttattga
     4561 tcaagcaaga ctgaaaatgc tagggcagac acgcggccgc atcttttacc catacgatgt
     4621 tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcca ccggatctag
     4681 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     4741 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     4801 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4861 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     4921 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     4981 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5041 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5101 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5161 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5221 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5281 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5341 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     5401 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5461 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     5521 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5821 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     5881 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6061 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     6121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6421 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6661 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     6721 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     6781 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6841 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     6901 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     6961 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     7021 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     7081 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     7141 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7201 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7261 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7381 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7441 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7501 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7561 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7621 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7681 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7741 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7801 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7861 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggc
//
