LOCUS       sequence-373908-        2507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses antiFtszsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha
            cell. For experiment use Dh5alphaz1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Mukhopadhyay S, Bagh S
  TITLE     A microgravity responsive synthetic genetic device in Escherichia 
            coli.
  JOURNAL   Biosens Bioelectron. 2020 Nov 1;167:112462. doi: 
            10.1016/j.bios.2020.112462. Epub 2020 Aug 1.
  PUBMED    32781386
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosens 
            Bioelectron."; date: "2020-11-1"; volume: "167:112462. doi"; pages: 
            " 10.1016/j.bios.2020.112462. Epub 2020 Aug 1"
COMMENT     Sequence Label: T_antiFtszsynsrRNA_UA
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      218..304
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      629..1217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1118..1137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1311..1405)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1435..2226,2227..2295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2058..2077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2296..2400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2468..2486
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(2507,1..18,19..24,25..41,42..47,48..73)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    join(2507,1..18)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cacatcagca
       61 ggacgcactg accgaattct aatcaccgcg tcattggtaa gttccattgg ttcaaacatt
      121 ttctgttggg ccattgcatt gccactgatt ttccaacata taaaaagaca agcccgaaca
      181 gtcgtccggg ctttttttct cgagcttcta gaggcatcaa ataaaacgaa aggctcagtc
      241 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
      301 aaatccgccg ccctagacct aggggctggc actctgtcga taccccaccg agaccccatt
      361 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
      421 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
      481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
      541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1261 ggccttttgc tcacatgttc tttcctgcgt tatcccctac tagtgcttgg attctcacca
     1321 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1381 attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagttacca
     1441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1561 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     2281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2401 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2461 ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagt
//
