LOCUS       sequence-373909-        2507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses antitdTomatosynsrRNA from PlTetO-1 promoter in E.coli 
            Dh5alpha cell. For experiment use Dh5alphaz1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Mukhopadhyay S, Bagh S
  TITLE     A microgravity responsive synthetic genetic device in Escherichia 
            coli.
  JOURNAL   Biosens Bioelectron. 2020 Nov 1;167:112462. doi: 
            10.1016/j.bios.2020.112462. Epub 2020 Aug 1.
  PUBMED    32781386
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosens 
            Bioelectron."; date: "2020-11-1"; volume: "167:112462. doi"; pages: 
            " 10.1016/j.bios.2020.112462. Epub 2020 Aug 1"
COMMENT     Sequence Label: T_antitdTomatosynsrRNA_UA
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(11..29,30..35,36..52,53..58,59..84)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      229..315
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      640..1228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1129..1148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1322..1416)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1446..2237,2238..2306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2069..2088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2307..2411)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2479..2497
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcacctcgag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
       61 gcacatcagc aggacgcact gaccgaattc gcatgaactc tttgatgacc tcctcgccct
      121 tgctcaccat tttctgttgg gccattgcat tgccactgat tttccaacat ataaaaagac
      181 aagcccgaac agtcgtccgg gctttttttc tcgagcttct agaggcatca aataaaacga
      241 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      301 ctgagtagga caaatccgcc gccctagacc taggggctgg cactctgtcg ataccccacc
      361 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
      421 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
      481 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      541 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      781 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1261 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatccccta ctagtgcttg
     1321 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1381 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta aacttggtct
     1441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1741 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2461 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtct
//
