LOCUS       sequence-37391-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, 
            Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, 
            Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Genetic engineering of human pluripotent cells using TALE nucleases.
  JOURNAL   Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
  PUBMED    21738127
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927."
COMMENT     Sequence Label: PITX3-2A-eGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(675..691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(675..691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(688..710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    699..715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(723..729,730..747,748..753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    769..790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tctgcccttc tacattggcc cgggattctc ttcgacatcc cctgcttgtt tcccagggag
       61 aagttagtgg cgctagctac gggcctttcc acggcgtact ggcacggact aaggttggct
      121 gccgggggcg gcccgtgcac agcggggtag ctgaaggagg cgtgctgttt ggctttgagc
      181 cgcaggctgg ccaggctcga gttacacggg tcccgataga cgtaggggga agaggcggca
      241 gccgcggcgg cggcggcggc cgaggcataa gggcaggaca cggccccgga ggacacggcg
      301 gccggagcca gcccgggggg gcccccgccc aggccctgca gggccccagg ccctggcacg
      361 gtgcccgggg cagccgcggc ggagggcacc atggaggcgg cgatggagct gggtggcgag
      421 aagacgggct gcgaagccag aggccccacg ttgaccgagt tgaaggcgaa tggaaaggtc
      481 ttggcggcga gcggcggggc aagagccttg ggcggccagt tgccgtacga gtagccgggg
      541 tacacctcct cgtagggcgg caccagcccc ccgagcggcg ccgcgaagct gcctttgcat
      601 agctcggcct gctggctgcg ctcgcgcttc cgccatttgg atccgagctc ggtaccaagc
      661 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      721 cacaacatac gagccggaag cataaagtgt aaaagcctgg ggtgcctaat gagtgagcta
      781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaaac ctgtcgtgcc
      841 agctgcatta atgaatcggc ccaacgcgcg gggaaaagcg gtttgcgtat tggtcgctc
//
