LOCUS       sequence-373910-        2507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses antiEGFPsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha
            cell. For experiment use Dh5alphaz1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Mukhopadhyay S, Bagh S
  TITLE     A microgravity responsive synthetic genetic device in Escherichia 
            coli.
  JOURNAL   Biosens Bioelectron. 2020 Nov 1;167:112462. doi: 
            10.1016/j.bios.2020.112462. Epub 2020 Aug 1.
  PUBMED    32781386
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosens 
            Bioelectron."; date: "2020-11-1"; volume: "167:112462. doi"; pages: 
            " 10.1016/j.bios.2020.112462. Epub 2020 Aug 1"
COMMENT     Sequence Label: T_antiEGFPsynsrRNA_UA
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(4..22,23..28,29..45,46..51,52..77)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    29..47
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      222..308
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      633..1221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1122..1141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1315..1409)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1439..2230,2231..2299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2062..2081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2300..2404)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2472..2490
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcacatc
       61 agcaggacgc actgaccgaa ttcgcaccac cccggtgaac agctcctcgc ccttgctcac
      121 cattttctgt tgggccattg cattgccact gattttccaa catataaaaa gacaagcccg
      181 aacagtcgtc cgggcttttt ttctcgagct tctagaggca tcaaataaaa cgaaaggctc
      241 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      301 ggacaaatcc gccgccctag acctaggggc tggcactctg tcgatacccc accgagaccc
      361 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
      421 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
      481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
      541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctactagtgc ttggattctc
     1321 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1381 ggtcattact ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt
     1441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2401 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     2461 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctc
//
