LOCUS       sequence-373944-        9967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Barcoded piggybac transposon vector with Dox-inducible expression of
            ZFPM2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, 
            Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda 
            T, Chatterjee P, Church GM
  TITLE     Directed differentiation of human iPSCs to functional ovarian 
            granulosa-like cells via transcription factor overexpression.
  JOURNAL   Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
  PUBMED    36803359
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-21"; volume: "12:e83291. doi"; pages: " 
            10.7554/eLife.83291"
COMMENT     Sequence Label: pPB-cT3G-cERP2-ZFPM2
FEATURES             Location/Qualifiers
     source          1..9967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3849..3873)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3875..3901
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     gap             3964..3983
                     /estimated_length=20
     polyA_signal    4068..4202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4474..4508
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4519..4536)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4519..4535)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4528..4550)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4744..4763)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4926..4944)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5012..5116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5117..5185,5186..5977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5335..5354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6148..6736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6637..6656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6890..6907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7113..7134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7149..7154,7155..7172,7173..7179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7187..7203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7192..7214
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7211..7227
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7211..7227
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7253..7315
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    7491..7572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8289..8345)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8355..9098)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(9099..9122)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9122..9131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9151..9362)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        9363..9742
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagttgg caccatgtcc cggcgaaagc aaagcaaacc
      421 ccggcagatc aaacggccgc ttgaagatgc cattgaagat gaggaagaag aatgtccatc
      481 agaggaaaca gacatcatct ccaaaggaga ctttccattg gaggaaagct tttccacaga
      541 atttgggcct gaaaatctga gctgcgaaga agtggaatac ttttgtaaca aaggtgatga
      601 tgaaggaatc caggagacag cagaatcaga tggggacaca cagtcagaga aaccggggca
      661 acctggagtt gagacagacg actgggatgg accaggagag ctggaggtgt ttcagaaaga
      721 tggggaacga aaaattcaga gtcgacagca acttccagtg ggaacaacct gggggccgtt
      781 tcctgggaag atggacttga ataataattc tttgaagaca aaggctcagg tcccaatggt
      841 gctgactgct ggtcccaagt ggttgctgga tgtgacttgg caaggagtgg aagacaacaa
      901 aaacaactgc attgtgtaca gcaaaggggg tcagctttgg tgtacaacta cgaaggccat
      961 ctctgagggt gaagagctaa ttgcctttgt ggtggatttt gactcaaggc tacaagctgc
     1021 cagtcagatg actctcacag aagggatgta ccctgcacgc ctgctggact caattcagct
     1081 gcttcctcag caagctgcca tggcttctat tttgcccaca gctattgtca ataaggatat
     1141 attcccttgc aagtcctgtg gcatctggta tcggagtgag cggaatctgc aggcccattt
     1201 gatgtactac tgcagtggga ggcaaagaga agctgctccg gtgtcagagg aaaatgaaga
     1261 cagtgcccat cagatttcca gcctgtgccc cttcccacag tgcaccaaga gcttttcaaa
     1321 tgctcgagct ctagaaatgc acctgaattc acacagtgga gtgaaaatgg aagaattcct
     1381 gccccctggt gctagtctaa aatgcaccgt ctgtagctac actgctgatt ccgtgatcaa
     1441 ctttcaccaa cacctgttct cccatctcac tcaagctgcc ttccgatgta atcactgcca
     1501 tttcggcttc cagactcaga gggagttatt gcagcaccag gagctccatg tccctagcgg
     1561 caaacttccc agagaaagtg acatggaaca ctctccaagt gcaactgaag acagcttaca
     1621 gccagccaca gacttattga ccagaagcga acttccccag agccaaaagg ccatgcagac
     1681 taaagatgcg agctctgaca cagagctgga caagtgtgag aaaaagactc agctctttct
     1741 cacgaaccag agaccagaga tacagcctac aacaaataaa caaagctttt cttacacaaa
     1801 aataaagtct gagccctcta gcccaagact tgcctcatct ccagttcagc ctaatattgg
     1861 gccttctttc cctgtgggcc ctttcctatc tcagttttct ttcccccaag atatcaccat
     1921 ggtccctcaa gcttcagaga tcttagctaa gatgtctgaa ctggtgcatc ggcgactgag
     1981 gcatggcagt agtagctacc ctcccgtcat ttacagccct ttgatgccca agggggctac
     2041 ttgttttgag tgtaacataa cattcaataa tttggataat tatctagtgc acaaaaagca
     2101 ttattgcagc agccgatggc agcagatggc taagtcccca gagttcccta gtgtgtcaga
     2161 aaagatgcct gaagctttga gtcccaacac tggccaaacc tccataaacc ttctcaaccc
     2221 agctgctcat tctgctgatc ctgagaatcc acttcttcaa acatcttgca tcaattcttc
     2281 cactgtctta gatttaattg ggccaaatgg gaagggccat gacaaggact tttccactca
     2341 aactaagaag ctctccacct ccagtaacaa tgatgacaaa attaatggaa aacctgttga
     2401 tgtgaaaaat cccagtgtcc ccttagtgga tggggaaagt gacccaaata agactacctg
     2461 tgaagcttgc aacattacct tcagccggca cgaaacatac atggtccaca aacagtatta
     2521 ctgtgctaca cgccacgacc ctccactgaa gaggtctgct tccaacaaag tgcctgccat
     2581 gcagagaacc atgcgcacac gcaagcgcag aaagatgtat gagatgtgcc tacctgagca
     2641 ggaacaaagg cctccactgg ttcagcagag atttcttgac gtagccaacc tcaataatcc
     2701 ttgtacctcc actcaagaac ccacagaagg gctaggagag tgctaccacc caagatgtga
     2761 tatctttcca ggaattgtct ctaaacactt ggaaacttct ctgacgatca acaagtgtgt
     2821 tccagtttcc aaatgtgata ctactcattc cagtgtttcc tgcctagaga tggacgtgcc
     2881 catagatctc agcaaaaagt gtttatctca gtctgagcgg acgaccacgt ctcccaaaag
     2941 gctgctggac tatcacgagt gcactgtgtg caagatcagt ttcaataagg tagaaaacta
     3001 tctggcccac aagcagaatt tctgcccggt tactgcacat cagcgtaatg acctgggtca
     3061 actggacggc aaagtgtttc cgaatccaga aagcgaacga aacagccctg atgtcagcta
     3121 cgaaagaagc ataataaaat gtgagaaaaa tgggaatttg aagcagcctt cccccaatgg
     3181 aaacttattt tcatcccacc tagcaaccct gcaaggcttg aaggtcttta gtgaagctgc
     3241 tcagctcatt gctacaaaag aagaaaacag acatttgttt cttccacaat gcctttaccc
     3301 tggagcaata aagaaagcaa aaggagccga ccagctttct ccatattatg gaatcaagcc
     3361 aagtgattat atttctggtt ctcttgtcat ccataacact gacatcgagc aaagcagaaa
     3421 tgcagaaaat gaatctccta aaggccaggc ttcctcaaat gggtgtgctg cgctgaagaa
     3481 agattctctg ccattgttgc ccaaaaatcg aggaatggta atagtgaatg gtggactgaa
     3541 acaagatgag agacctgctg ccaacccaca gcaagagaac atttcccaga atcctcagca
     3601 cgaagacgac cacaaatctc cctcgtggat ctctgagaac ccattagctg ccaatgagaa
     3661 tgtctcacca ggaattccct cagcagagga acagttgtct agtatagcaa aaggtgtgaa
     3721 tggttccagc caggctccaa ccagtgggaa atattgccgg ctatgtgata tccagttcaa
     3781 caacctttca aactttataa ctcacaagaa gttttattgc tcatcacatg cagcagaaca
     3841 tgtcaaattg ccagctttct tgtacaaagt ggtataccca tacgatgttc cagattacgc
     3901 ttagtaatga gaaatactca gaagatgtca ctcaccatag tccgcagtga aagataaatg
     3961 atcnnnnnnn nnnnnnnnnn nnngttttag agctagaaat agcaagtagg ccactgagca
     4021 agaactatga atgagatctt tagacatatt gcggccgctt tctccgcgat ccagacatga
     4081 taagatacat tgatgagttt ggacaaacca aaactagaat gcagtgaaaa aaatgcctta
     4141 tttgtgaaat ttgtgatgct attgccttat ttgtaaccat tataagctgc aataaacaag
     4201 ttatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
     4261 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
     4321 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
     4381 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
     4441 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
     4501 ttctagggtt aagaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4561 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4621 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     4681 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     4741 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4801 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccat
     4861 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaaca cgcgagttcg
     4921 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4981 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5041 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5101 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5161 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5221 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5281 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5341 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5401 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5461 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5521 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5581 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5641 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5701 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5761 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5821 cggtgagcgt ggttcacgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5881 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5941 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6001 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6061 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6781 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6841 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6901 gagcgaggaa gcggatgagc gcccaatacg caaaccgcct ctcaccgcgc gttggccgat
     6961 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7021 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7081 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7141 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7201 caatttcaca caggaaacag ctatgaccat gattacgcca aggtcgactt aaccctagaa
     7261 agataatcat attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt
     7321 tatcggtctg tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat
     7381 ttacacttac atactaataa taaattcaac aaacaattta tttatgttta tttatttatt
     7441 aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac aaaactttta gcagtgaaaa
     7501 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7561 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     7621 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     7681 ctggagattc atgcgccagg ttttctggtc atgcaccacg ttcgtgggcc ctcaggaacc
     7741 tccacgtcag cggtaactgt gaagcccagt cgctcgtaaa aaggaagatt ccggggagca
     7801 ctggtttcga ggaacgccgg cacgccagcc cgttccgcgg cctcaacacc aggaagaacg
     7861 acagcgcttc ccaaaccttt accttggtga tctgggctga caccgacagt ggcaagaaac
     7921 cacgcaggct cctttggtct atgaggagcc agcaagccct ccatttgctg ttgtgccgcc
     7981 aacctactcc ctgacagctc ggccatccgc ggacctattt ccgcgaacac tgcgccggct
     8041 tcgacgcttt ccggagtagt ccagaccgca actgcggcac catcgtccgc aacccaaact
     8101 ttaccaatgt ccagacccac gcgcgtaaga aacagctctt gcaactcagt taccctctct
     8161 atgtgtctgt ccggatctac ggtatggcgg gtcgctggat aatctgcaaa tgctgcggca
     8221 agtgttcgga cagcccgtgg tacatcgtca cgggtagcga gcctaacagt gggcttatat
     8281 tccgtcatgg ggccggggtt ctcctccacg tcgccggcct gcttcagcag gctgaagttg
     8341 gtggcgccgc tgcccccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
     8401 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
     8461 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
     8521 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
     8581 gcttgtttca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
     8641 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
     8701 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
     8761 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcacga
     8821 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
     8881 tcgccttcca gggggcagga gtgggtatga tgcctgtcca gcatctcgat tggcagggca
     8941 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
     9001 ttttgagcga gtttccttgt cgtcaggcct tcgataccga ctccattgag taattccaga
     9061 gcagagttta tgactttgct cttgtccagt ctagacatct tatcgtcatc gtctttgtaa
     9121 tccatggtgg cggatcccgc gtcacgacac ctgtgttctg gcggcaaacc cgttgcgaaa
     9181 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
     9241 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
     9301 ggttcaattg ccgacccctc cccccaactt ctcgggcact gtgggccatg tgcgctctgc
     9361 cccatggtaa tagccatgac taatacgtag atgtactgcc aagtaggaaa gtgccataag
     9421 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     9481 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     9541 acccattgac gtcaatggaa agtgcctatt ggcgttacta tgggaacata cgtcattatt
     9601 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     9661 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     9721 taactagtca ataatcaatg tcataagctc taatcgcata atcgagctaa gagcgattaa
     9781 tatgcttagc tagcttagct aagctagatt tctctattct cccacaatat atagcagcta
     9841 ttagcttagc agtccgaata cgatcgtcca cgatccataa gcgtaacaca tttgagtagc
     9901 gactgacggg caccggagcc actcgagtgg aattactgac gggcaccgga gccactcgag
     9961 tggaatt
//
