LOCUS       sequence-373951-        9140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intron-based gene-break transposon (GBT) for an effective and 
            revertible loss-of-function tool for zebrafish. Ubiquitous GFP 
            frameshift 3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Ichino N, Serres MR, Urban RM, Urban MD, Treichel AJ, Schaefbauer 
            KJ, Greif LE, Varshney GK, Skuster KJ, McNulty MS, Daby CL, Wang Y, 
            Liao HK, El-Rass S, Ding Y, Liu W, Anderson JL, Wishman MD, 
            Sabharwal A, Schimmenti LA, Sivasubbu S, Balciunas D, Hammerschmidt 
            M, Farber SA, Wen XY, Xu X, McGrail M, Essner JJ, Burgess SM, Clark 
            KJ, Ekker SC
  TITLE     Building the vertebrate codex using the gene breaking protein trap 
            library.
  JOURNAL   Elife. 2020 Aug 11;9. pii: 54572. doi: 10.7554/eLife.54572.
  PUBMED    32779569
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-08-11"; volume: "9. pii: 54572. doi"; pages: " 
            10.7554/eLife.54572"
COMMENT     Sequence Label: pGBT-RP2.3
FEATURES             Location/Qualifiers
     source          1..9140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..43
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(58..63,64..81,82..88)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     101..123
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     120..136
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     120..136
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        154..172
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     154..171
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(223..234,235..397)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(448..481)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             651..1325
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             651..1325
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(778..796)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1046..1065
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1240..1263
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     2575..2595
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2577..2595
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5186..5899
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5216..5244)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5839..5859
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(6111..6144)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(6223..6410,6411..6422)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6434..6453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6435..6453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6460..6477)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6460..6476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6469..6491)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6617..7072
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6704..6723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6914..6935
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7150..7254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7255..7323,7324..8115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7473..7492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8286..8874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8775..8794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9028..9045
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
       61 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      121 aggaaacagc tatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa
      181 gctatgcatc caacgcgttg ggagctctcc catatggtcg agcagaggtg taaaaagtac
      241 tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag
      301 taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg
      361 tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta
      421 agctcatggt agggataaca gggtaatata acttcgtata gcatacatta tacgaagtta
      481 tcgttaccac ccactagcgg tcagactgca gattgcagca cgaaacagga agctgactcc
      541 acatggtcac atgctcactg aagtgttgac ttccctgaca gctgtgcact ttctaaaccg
      601 gttttctcat tcatttacag cagccggaat tcacccgggt accggaaggt gcctcctccg
      661 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
      721 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      781 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      841 tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      901 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      961 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     1021 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     1081 cctccaccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc
     1141 tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga
     1201 agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca
     1261 acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg
     1321 cctaagaaat cgctagtaat taagtctcag ccaccgttaa ctgaacatgt caaaacctgt
     1381 ggagactgtt gagatttgat gttctgaaaa gataaagcct ataaataaaa tgttgcccaa
     1441 atttcctgcc tgatgttttt ctttgtcttt gctacatggc tttgctgctc ggatcggctc
     1501 actctgtgta tgccacgttc actttgtact ctccttctca cggtaggttt attattttta
     1561 gatgtgcagt tagtttctgt gaaataacac accacacact gatattgtct gtgcattgac
     1621 ttggtgagtg cacattgttt ttgatcttga catatttata tttgattgat caggtgaact
     1681 gtgtgaatct aaagtgctcc atacagatgt tctgcattga aaatattctc attttattag
     1741 tggaagtgag tgtatgccac atccaatcaa tttcagcaaa caccccagta tgatttaatg
     1801 caaaaaaatg aaggtatcaa acacgcatta ctactttgca gttaaatatt taacatttat
     1861 tccaacacga aaaaaagcag taaataacac tttgacaaac acgtcaggac atcttatttt
     1921 tgtcaccctc acaggcaatt tagtataata tattatatat atatatatat catataataa
     1981 tattcagtat aatatatata tatatatcat attataatat tcagtataat ataaaacaca
     2041 aacacatata tgtataatat aatataacat ttttatttat tgagatgcct ctatggaccg
     2101 tgttataaga agtaaagatc aggagaagta aacatgaagt gtaattatga atactgatgt
     2161 taaattaagc tatgatgagt tttcactgtt aatttaccat ctcaattaaa tgttgatgcc
     2221 tccatgacca agttaagcag atgagactga ggacaactgt agaagacaag atgttcactt
     2281 tgctgaatat agctggcttg acagttatct atgactctat aaatatatat atattttttt
     2341 ttttataaaa tgatttattt ataactatat atccatttct cagacaggtg cttcatatcc
     2401 ctcactcccg tagctgtcca tgctggatct gtccccgttg tttttaaaaa gctaaataag
     2461 ttattaacat gactgcatcc agcgagccaa acctgtctgg tgtacagcta ccagagaagc
     2521 ttgagatcct aggaagttcc tatactttct agagaatagg aatcgcgagt gctagcaatt
     2581 aaccctcact aaagggaaca aaagctttta gaccttctta cttttgggga ttatataagt
     2641 attttctcaa taaatatctc atatcttact gtggtttaac tgctgaatct aaaattttaa
     2701 tacaaaagta gttatatttg ttgtacattg taaactataa cttaacttca gtttcagaga
     2761 aactcatgtg ctcaaaatgt aaaaaaagtt tcctgttaaa tattttgtaa atgtattgaa
     2821 gacaaaataa gaaaaaaaaa aatataagcc actaaatcac actgtccttg gtatcagcaa
     2881 gagattctga cataatcagc tgtttttgtt tattactgcc attgaaggcc atgtgcatta
     2941 gtcccaagtt acacattaaa aagtcacatg tagcttacca acatcagtgc tgttcaagca
     3001 cagcctcatc tactattcaa actgtggcac catctaaaat atgccagaat ttttttattt
     3061 aatgaatttg accctgaaat atgtattaat atcactcctg tgattttttt gtaatcagct
     3121 tacaattaca ggaatgcaag cctgattcat tacaagtttc actacacttt ctctgacaac
     3181 atcacctact gaactcagac cagctagttg ctccttaagt atacaatcat gtcactaatc
     3241 ctcatttcaa tgaaaaatac ccctattgta cttggtactt ggtagataac cacagagcag
     3301 tattatgcca ttattgtgaa tacaataaga ggtaaatgac ctacagagct gctgctgctg
     3361 ttgtgttaga ttgtaaacac agcacaggat caaggaggtg tccatcacta tgaccaatac
     3421 tagcactttg cacaggctct ttgaaaggct gaaaagagcc ttattggcgt tatcacaaca
     3481 aaatacgcaa atacggaaaa caacgtattg aacttcgcaa acaaaaaaca gcgattttga
     3541 tgaaaatcgc ttaggccttg ctcttcaaac aatccagctt ctccttcttt cactctcaag
     3601 ttgcaagaag caagtgtagc aatgtgcacg cgacagccgg gtgtgtgacg ctggaccaat
     3661 cagagcgcag agctccgaaa gtttaccttt tatggctaga gccgggcatc tgccgtcata
     3721 taaaagagcg cgcccagcgt ctcagcctca ctttgagctc ctccacacgc agctagtgcg
     3781 gaatatcatc tgcctgtaac ccattctcta aagtcgacaa acccccccaa acctaaggtg
     3841 agttgatctt taagcttttt acattttcag ctcgcatata tcaattcgaa cgtttaatta
     3901 gaatgtttaa ataaagctag attaaatgat taggctcagt taccggtctt ttttttctca
     3961 tttacgtgcg aactctgctt aaactctagt tattctttat taatatgtgg ttatttttat
     4021 atatgtatgt gttatcataa ctgtactggc tatgtcaggt ggtaatgact gtaacgttac
     4081 gttactcgtt gtaggcacga cattgaatgg gccggtgttg aaataagtct tcaacccctt
     4141 ttaacctcaa aatgtgctct ggttaacaag gattttaaca gctatcagta tgactgtgcg
     4201 gttttaaagc cgttagtgag gcacgttgca cacttgatgg atggccggaa tgggaagttc
     4261 tttatgcagg cagtgctgca gcagggtgtg acctacttta gctaacgtta gccggctaac
     4321 cagcattcat ctgccggtaa cttgagtcta atattctctc atgtgatatc gaagtgatca
     4381 aagacacgtc tgttagctca ctttaaccaa ctgtagtgaa aaatagcgca gtgtgcagcc
     4441 cttcagtctt tcatttaggc tgattattca atcattttat taactattaa cgcgttacta
     4501 aacgtaaggt aacgtagtca gtttttaata actggtgaaa agtactggtt gggtttaaat
     4561 ggtgacttat aattgtgttg gagggggaaa cctttttgat aaaggctata taatctcaaa
     4621 tgaatgggct gaggatggtg ttcacaggtg ctttagtgaa gtccgctcgt gaagagtcgc
     4681 tgaagtgact gcagatctgt gacgcagtcg ttttgggcag acggccgttg aaattcggtt
     4741 gagtaattga taccaggtga ggctagagga tgtagaaatt catttgtgta gaatttaggg
     4801 agtggccctg gcgtgatgaa tgtcgaaatc cgttcctttt tactgaaccc tatgtctctg
     4861 gctgagtgcc acaccgccgg cagccgcaaa gcgtctcaaa ccattgcctt ttatggtaat
     4921 aatgagaatg cagagggact tcctttgtct ggcacatctg aggcgcgcat tgtcacacta
     4981 gcacccacta gcggtcagac tgcagattgc agcacgaaac aggaagctga ctccacatgg
     5041 tcacatgctc actgaagtgt tgacttccct gacagctgtg cactttctaa accggttttc
     5101 tcattcattt acagttcagc cggaattcac ctgcaggtgg atccatctag aactactaga
     5161 ggatccccgg gtaccggtag aaaaaatgag taaaggagaa gaacttttca ctggagttgt
     5221 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     5281 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     5341 actacctgtt ccatggccaa cacttgtcac tactttcgcg tatggtcttc aatgctttgc
     5401 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
     5461 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
     5521 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     5581 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
     5641 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
     5701 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     5761 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
     5821 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     5881 catggatgaa ctatacaaat aaatgtccag acctgcagga attcgatatc aagcttatcg
     5941 atgaacaaac ccccccaaac ctaaggtgag ttgatcttta agctttttac attttcagct
     6001 cgcatatatc aattcgaacg tttaattaga atgtttaaat aaagctagat taaatgatta
     6061 ggctcagtta ccggtctttt ttttctcatt tacactagct aagcatgctg ataacttcgt
     6121 atagcataca ttatacgaag ttattaccct gttatcccta caaattaaac tgggcatcag
     6181 cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat
     6241 aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa
     6301 aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat
     6361 ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc
     6421 tgggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     6481 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6541 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6601 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6661 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6721 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6781 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6841 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6901 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6961 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7021 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     7081 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg
     7141 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7201 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7261 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7321 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7381 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7441 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7501 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7561 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7621 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7681 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7741 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7801 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7861 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7921 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7981 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8041 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8101 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8161 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8221 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8401 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8641 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8881 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8941 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9001 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9061 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9121 aggtttcccg actggaaagc
//
