LOCUS       sequence-373952-        9141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intron-based gene-break transposon (GBT) for an effective and 
            revertible loss-of-function tool for zebrafish. Ubiquitous GFP 
            frameshift 2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Ichino N, Serres MR, Urban RM, Urban MD, Treichel AJ, Schaefbauer 
            KJ, Greif LE, Varshney GK, Skuster KJ, McNulty MS, Daby CL, Wang Y, 
            Liao HK, El-Rass S, Ding Y, Liu W, Anderson JL, Wishman MD, 
            Sabharwal A, Schimmenti LA, Sivasubbu S, Balciunas D, Hammerschmidt 
            M, Farber SA, Wen XY, Xu X, McGrail M, Essner JJ, Burgess SM, Clark 
            KJ, Ekker SC
  TITLE     Building the vertebrate codex using the gene breaking protein trap 
            library.
  JOURNAL   Elife. 2020 Aug 11;9. pii: 54572. doi: 10.7554/eLife.54572.
  PUBMED    32779569
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-08-11"; volume: "9. pii: 54572. doi"; pages: " 
            10.7554/eLife.54572"
COMMENT     Sequence Label: pGBT-RP2.2
FEATURES             Location/Qualifiers
     source          1..9141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..43
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(58..63,64..81,82..88)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     101..123
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     120..136
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     120..136
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        154..172
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     154..171
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(223..234,235..397)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(448..481)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             652..1326
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             652..1326
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(779..797)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1047..1066
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1241..1264
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     2576..2596
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2578..2596
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5187..5900
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5217..5245)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5840..5860
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(6112..6145)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(6224..6411,6412..6423)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6435..6454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6436..6454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6461..6478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6461..6477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6470..6492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6618..7073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6705..6724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6915..6936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7151..7255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7256..7324,7325..8116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7474..7493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8287..8875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8776..8795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9029..9046
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
       61 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      121 aggaaacagc tatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa
      181 gctatgcatc caacgcgttg ggagctctcc catatggtcg agcagaggtg taaaaagtac
      241 tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag
      301 taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg
      361 tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta
      421 agctcatggt agggataaca gggtaatata acttcgtata gcatacatta tacgaagtta
      481 tcgttaccac ccactagcgg tcagactgca gattgcagca cgaaacagga agctgactcc
      541 acatggtcac atgctcactg aagtgttgac ttccctgaca gctgtgcact ttctaaaccg
      601 gttttctcat tcatttacag tcagccggaa ttcacccggg taccggaagg tgcctcctcc
      661 gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
      721 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      781 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctcag
      841 ttccagtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      901 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      961 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     1021 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1081 gcctccaccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagatgagg
     1141 ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta catggccaag
     1201 aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat cacctcccac
     1261 aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc
     1321 gcctaagaaa tcgctagtaa ttaagtctca gccaccgtta actgaacatg tcaaaacctg
     1381 tggagactgt tgagatttga tgttctgaaa agataaagcc tataaataaa atgttgccca
     1441 aatttcctgc ctgatgtttt tctttgtctt tgctacatgg ctttgctgct cggatcggct
     1501 cactctgtgt atgccacgtt cactttgtac tctccttctc acggtaggtt tattattttt
     1561 agatgtgcag ttagtttctg tgaaataaca caccacacac tgatattgtc tgtgcattga
     1621 cttggtgagt gcacattgtt tttgatcttg acatatttat atttgattga tcaggtgaac
     1681 tgtgtgaatc taaagtgctc catacagatg ttctgcattg aaaatattct cattttatta
     1741 gtggaagtga gtgtatgcca catccaatca atttcagcaa acaccccagt atgatttaat
     1801 gcaaaaaaat gaaggtatca aacacgcatt actactttgc agttaaatat ttaacattta
     1861 ttccaacacg aaaaaaagca gtaaataaca ctttgacaaa cacgtcagga catcttattt
     1921 ttgtcaccct cacaggcaat ttagtataat atattatata tatatatata tcatataata
     1981 atattcagta taatatatat atatatatca tattataata ttcagtataa tataaaacac
     2041 aaacacatat atgtataata taatataaca tttttattta ttgagatgcc tctatggacc
     2101 gtgttataag aagtaaagat caggagaagt aaacatgaag tgtaattatg aatactgatg
     2161 ttaaattaag ctatgatgag ttttcactgt taatttacca tctcaattaa atgttgatgc
     2221 ctccatgacc aagttaagca gatgagactg aggacaactg tagaagacaa gatgttcact
     2281 ttgctgaata tagctggctt gacagttatc tatgactcta taaatatata tatatttttt
     2341 tttttataaa atgatttatt tataactata tatccatttc tcagacaggt gcttcatatc
     2401 cctcactccc gtagctgtcc atgctggatc tgtccccgtt gtttttaaaa agctaaataa
     2461 gttattaaca tgactgcatc cagcgagcca aacctgtctg gtgtacagct accagagaag
     2521 cttgagatcc taggaagttc ctatactttc tagagaatag gaatcgcgag tgctagcaat
     2581 taaccctcac taaagggaac aaaagctttt agaccttctt acttttgggg attatataag
     2641 tattttctca ataaatatct catatcttac tgtggtttaa ctgctgaatc taaaatttta
     2701 atacaaaagt agttatattt gttgtacatt gtaaactata acttaacttc agtttcagag
     2761 aaactcatgt gctcaaaatg taaaaaaagt ttcctgttaa atattttgta aatgtattga
     2821 agacaaaata agaaaaaaaa aaatataagc cactaaatca cactgtcctt ggtatcagca
     2881 agagattctg acataatcag ctgtttttgt ttattactgc cattgaaggc catgtgcatt
     2941 agtcccaagt tacacattaa aaagtcacat gtagcttacc aacatcagtg ctgttcaagc
     3001 acagcctcat ctactattca aactgtggca ccatctaaaa tatgccagaa tttttttatt
     3061 taatgaattt gaccctgaaa tatgtattaa tatcactcct gtgatttttt tgtaatcagc
     3121 ttacaattac aggaatgcaa gcctgattca ttacaagttt cactacactt tctctgacaa
     3181 catcacctac tgaactcaga ccagctagtt gctccttaag tatacaatca tgtcactaat
     3241 cctcatttca atgaaaaata cccctattgt acttggtact tggtagataa ccacagagca
     3301 gtattatgcc attattgtga atacaataag aggtaaatga cctacagagc tgctgctgct
     3361 gttgtgttag attgtaaaca cagcacagga tcaaggaggt gtccatcact atgaccaata
     3421 ctagcacttt gcacaggctc tttgaaaggc tgaaaagagc cttattggcg ttatcacaac
     3481 aaaatacgca aatacggaaa acaacgtatt gaacttcgca aacaaaaaac agcgattttg
     3541 atgaaaatcg cttaggcctt gctcttcaaa caatccagct tctccttctt tcactctcaa
     3601 gttgcaagaa gcaagtgtag caatgtgcac gcgacagccg ggtgtgtgac gctggaccaa
     3661 tcagagcgca gagctccgaa agtttacctt ttatggctag agccgggcat ctgccgtcat
     3721 ataaaagagc gcgcccagcg tctcagcctc actttgagct cctccacacg cagctagtgc
     3781 ggaatatcat ctgcctgtaa cccattctct aaagtcgaca aaccccccca aacctaaggt
     3841 gagttgatct ttaagctttt tacattttca gctcgcatat atcaattcga acgtttaatt
     3901 agaatgttta aataaagcta gattaaatga ttaggctcag ttaccggtct tttttttctc
     3961 atttacgtgc gaactctgct taaactctag ttattcttta ttaatatgtg gttattttta
     4021 tatatgtatg tgttatcata actgtactgg ctatgtcagg tggtaatgac tgtaacgtta
     4081 cgttactcgt tgtaggcacg acattgaatg ggccggtgtt gaaataagtc ttcaacccct
     4141 tttaacctca aaatgtgctc tggttaacaa ggattttaac agctatcagt atgactgtgc
     4201 ggttttaaag ccgttagtga ggcacgttgc acacttgatg gatggccgga atgggaagtt
     4261 ctttatgcag gcagtgctgc agcagggtgt gacctacttt agctaacgtt agccggctaa
     4321 ccagcattca tctgccggta acttgagtct aatattctct catgtgatat cgaagtgatc
     4381 aaagacacgt ctgttagctc actttaacca actgtagtga aaaatagcgc agtgtgcagc
     4441 ccttcagtct ttcatttagg ctgattattc aatcatttta ttaactatta acgcgttact
     4501 aaacgtaagg taacgtagtc agtttttaat aactggtgaa aagtactggt tgggtttaaa
     4561 tggtgactta taattgtgtt ggagggggaa acctttttga taaaggctat ataatctcaa
     4621 atgaatgggc tgaggatggt gttcacaggt gctttagtga agtccgctcg tgaagagtcg
     4681 ctgaagtgac tgcagatctg tgacgcagtc gttttgggca gacggccgtt gaaattcggt
     4741 tgagtaattg ataccaggtg aggctagagg atgtagaaat tcatttgtgt agaatttagg
     4801 gagtggccct ggcgtgatga atgtcgaaat ccgttccttt ttactgaacc ctatgtctct
     4861 ggctgagtgc cacaccgccg gcagccgcaa agcgtctcaa accattgcct tttatggtaa
     4921 taatgagaat gcagagggac ttcctttgtc tggcacatct gaggcgcgca ttgtcacact
     4981 agcacccact agcggtcaga ctgcagattg cagcacgaaa caggaagctg actccacatg
     5041 gtcacatgct cactgaagtg ttgacttccc tgacagctgt gcactttcta aaccggtttt
     5101 ctcattcatt tacagttcag ccggaattca cctgcaggtg gatccatcta gaactactag
     5161 aggatccccg ggtaccggta gaaaaaatga gtaaaggaga agaacttttc actggagttg
     5221 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     5281 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     5341 aactacctgt tccatggcca acacttgtca ctactttcgc gtatggtctt caatgctttg
     5401 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     5461 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     5521 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     5581 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     5641 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     5701 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     5761 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     5821 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     5881 gcatggatga actatacaaa taaatgtcca gacctgcagg aattcgatat caagcttatc
     5941 gatgaacaaa cccccccaaa cctaaggtga gttgatcttt aagcttttta cattttcagc
     6001 tcgcatatat caattcgaac gtttaattag aatgtttaaa taaagctaga ttaaatgatt
     6061 aggctcagtt accggtcttt tttttctcat ttacactagc taagcatgct gataacttcg
     6121 tatagcatac attatacgaa gttattaccc tgttatccct acaaattaaa ctgggcatca
     6181 gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat ctccaaaaaa
     6241 taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt ttttctaaaa
     6301 aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt aaagtaaaaa
     6361 tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta ctttacacct
     6421 ctgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     6481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6601 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6961 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7021 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
     7081 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg
     7141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7261 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7321 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7381 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7441 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7501 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7561 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7621 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7681 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7741 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7801 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7861 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7921 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7981 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8041 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8101 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9061 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9121 caggtttccc gactggaaag c
//
