LOCUS       sequence-373969-        5075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NDUFS6 Complex I sub-unit with C-terminus mVenus tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Balderas E, Eberhardt DR, Lee S, Pleinis JM, Sommakia S, Balynas AM,
            Yin X, Parker MC, Maguire CT, Cho S, Szulik MW, Bakhtina A, Bia RD, 
            Friederich MW, Locke TM, Van Hove JLK, Drakos SG, Sancak Y, 
            Tristani-Firouzi M, Franklin S, Rodan AR, Chaudhuri D
  TITLE     Mitochondrial calcium uniporter stabilization preserves energetic 
            homeostasis during Complex I impairment.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2769. doi: 10.1038/s41467-022-30236-4.
  PUBMED    35589699
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2769. doi"; pages: " 
            10.1038/s41467-022-30236-4"
COMMENT     Sequence Label: pEGFP-NDUFS6-mVenus
FEATURES             Location/Qualifiers
     source          1..5075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(69..863)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(69..863)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(180..199)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     790..809
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(898..1255)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      912..1047
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1064..1255
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     1212..1232
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(1257..1361)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1388..1843
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1475..1494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1685..1706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1850..1971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1861..1880)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1915..1934
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2101..2811)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2143..2164)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2492..2511
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2751..2772
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3218..3227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3222..3231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3266..3469)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3295..3315)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3470..3773
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3948..4536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4028..4047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4865..4912
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4937..4956
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
       61 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
      121 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
      181 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
      241 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
      301 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
      361 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
      421 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
      481 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
      541 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
      601 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
      661 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
      721 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
      781 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
      841 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
      901 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
      961 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     1021 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     1081 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     1141 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     1201 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     1261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1381 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1441 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1501 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1561 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1621 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1681 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1741 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1801 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttacgcgtt aagatacatt
     1861 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1921 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1981 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     2041 taaaacctct acaaatgtgg tatggctgat tatgatcagt tatctagatc cggtggatcc
     2101 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     2161 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtagc tcaggtagtg
     2221 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     2281 gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt
     2341 cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     2401 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     2461 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     2521 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     2581 catgtggtcg gggtagcggg cgaagcactg caggccgtag ccgagggtgg tcacgagggt
     2641 gggccagggc acgggcagct tgccggtggt gcagatgagc ttcagggtca gcttgccgta
     2701 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     2761 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tggtaccgtc
     2821 gactgcagaa tttgatatcc caccaatgtt taagtggtgg tgctgtctga actggagccc
     2881 acagtaaccg catgtgccgg tttttgtttc tttgtccaag tttatataca cttttgggtg
     2941 gccaagagct cccccgccgc catcgcacgc tatcacccga gtctccacct cgctcacggg
     3001 ctgctctgct atcaaatcaa tggcaaagtt ttcattcacc tctttctgac gacctacaaa
     3061 ccgaattctc ctgtagtctt tatcatcata aacctggcca gtgtgcgtga ccttctcccc
     3121 ggtcggcgag acccgcaccc cgaaacacct ggcgcccagg ggcaggctcc gcgccgcctc
     3181 gccacaccgg ttcagcagcc ggcagaaggt catcgccgcc gccatggtgg ctctagagtc
     3241 ggcggatctg acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
     3301 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     3361 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     3421 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     3481 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     3541 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     3601 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     3661 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     3721 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     3781 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaatgcatg
     3841 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3901 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3961 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4021 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4081 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4141 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4201 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4261 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4321 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4381 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4441 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4501 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4561 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4621 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4681 atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct
     4741 gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg
     4801 ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt
     4861 ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg
     4921 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg
     4981 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg
     5041 attccgaagc ccaacctttc atagaaggcg gcggt
//
