LOCUS       sequence-373983-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone that confers Kanamycin resistance, contains cloDF ori.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Wandera KG, Alkhnbashi OS, Bassett HVI, Mitrofanov A, Hauns S, Migur
            A, Backofen R, Beisel CL
  TITLE     Anti-CRISPR prediction using deep learning reveals an inhibitor of 
            Cas13b nucleases.
  JOURNAL   Mol Cell. 2022 May 24. pii: S1097-2765(22)00437-3. doi: 
            10.1016/j.molcel.2022.05.003.
  PUBMED    35649413
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 24. pii: S1097-2765(22)00437-3. doi: 
            10.1016/j.molcel.2022.05.003."
COMMENT     Sequence Label: pcloDF-kan
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     219..238
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      380..474
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1170..1908
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(1966..1984)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2163..2608,1..349)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2217..2236)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
       61 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
      121 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
      181 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
      241 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
      301 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
      361 gggttcgaga gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat
      421 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
      481 actagggaca gtaagacggg taagcctgtt gatgataccg ctgccttact gggtgcatta
      541 gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa aatcagaggg
      601 caggaactgc tgaacagcaa aaagtcagat agcaccacat agcagacccg ccataaaacg
      661 ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg catgaatcca
      721 taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga gcgcagcgaa
      781 ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca aaaggaatat
      841 tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt taaggtctgt
      901 tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga actgactaaa
      961 gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat tctttcttta
     1021 ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt ctagcggaat
     1081 ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag aatttacaga
     1141 tacccacaac tcaaaggaaa aggactagtg atcaaaggat cttcttgaga tccttttttt
     1201 ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa
     1261 ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac
     1321 caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca accgcgtgtt
     1381 tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag tggcgtttta
     1441 ccgtgctttt ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc
     1501 tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga
     1561 taccagtgtg tgagctatga gaaagcgcca cacttcccgt aagggagaaa ggcggaacag
     1621 gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac ccgccggaaa
     1681 cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc
     1741 tcacggctcc cacagatgca ccggaaaagc gtctgtttat gtgaactctg gcaggagggc
     1801 ggagcctatg gaaaaacgcc accggcgcgg ccctgctgtt ttgcctcaca tgttagtccc
     1861 ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc cgcagcgccc taggtctagg
     1921 gcggcggatt tgtcctactc aggaactcga ggtgaagacg aaagggcctc gtgatacgcc
     1981 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcggaa ttgccagctg
     2041 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     2101 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc
     2161 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     2221 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     2281 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     2341 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     2401 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     2461 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     2521 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     2581 gcatcgagcg agcacgtact cggatgga
//
