LOCUS       sequence-373993-       14531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDC42BPA (human) tagged with C-terminal
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14531)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 14531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-CDC42BPA-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..14531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10510..10534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10536..10577
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10584..11354
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(11360..11380)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    11409..11997
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11462..11482)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11880..11891)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        12011..12510
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12046..12069)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     12443..12462
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             12531..13130
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12531..12550)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13027..13047
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             13268..13501
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13573..13694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13610..13629)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13664..13683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13734..13869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13889..13908)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13890..13908)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13918..13935)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13918..13934)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13927..13949)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14076..14504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14163..14182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14373..14394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatgtctgg agaagtgcgt ttgaggcagt
     5461 tggagcagtt tattttggac gggcccgctc agaccaatgg gcagtgcttc agtgtggaga
     5521 cattactgga tatactcatc tgcctttatg atgaatgcaa taattctcca ttgagaagag
     5581 agaagaacat tctcgaatac ctagaatggg ctaaaccatt tacttctaaa gtgaaacaaa
     5641 tgcgattaca tagagaagac tttgaaatat taaaggtgat tggtcgagga gcttttgggg
     5701 aggttgctgt agtaaaacta aaaaatgcag ataaagtgtt tgccatgaaa atattgaata
     5761 aatgggaaat gctgaaaaga gctgagacag catgttttcg tgaagaaagg gatgtattag
     5821 tgaatggaga caataaatgg attacaacct tgcactatgc tttccaggat gacaataact
     5881 tatacctggt tatggattat tatgttggtg gggatttgct tactctactc agcaaatttg
     5941 aagatagatt gcctgaagat atggctagat tttacttggc tgagatggtg atagcaattg
     6001 actcagttca tcagctacat tatgtacaca gagacattaa acctgacaat atactgatgg
     6061 atatgaatgg acatattcgg ttagcagatt ttggttcttg tctgaagctg atggaagatg
     6121 gaacggttca gtcctcagtg gctgtaggaa ctccagatta tatctctcct gaaatccttc
     6181 aagccatgga agatggaaaa gggagatatg gacctgaatg tgactggtgg tctttggggg
     6241 tctgtatgta tgaaatgctt tacggagaaa caccatttta tgcagaatcg ctggtggaga
     6301 catacggaaa aatcatgaac cacaaagaga ggtttcagtt tccagcccaa gtgactgatg
     6361 tgtctgaaaa tgctaaggat cttattcgaa ggctcatttg tagcagagaa catcgacttg
     6421 gtcaaaatgg aatagaagac tttaagaaac acccattttt cagtggaatt gattgggata
     6481 atattcggaa ctgtgaagca ccttatattc cagaagttag tagcccaaca gatacatcga
     6541 attttgatgt agatgatgat tgtttaaaaa attctgaaac gatgccccca ccaacacata
     6601 ctgcattttc tggccaccat ctgccatttg ttggttttac atatactagt agctgtgtac
     6661 tttctgatcg gagctgttta agagttacgg ctggtcccac ctcactggat cttgatgtta
     6721 atgttcagag gactctagac aacaacttag caactgaagc ttatgaaaga agaattaagc
     6781 gccttgagca agaaaaactt gaactcagta gaaaacttca agagtcaaca cagactgtcc
     6841 aagctctgca gtattcaact gttgatggtc cactaacagc aagcaaagat ttagaaataa
     6901 aaaacttaaa agaagaaatt gaaaaactaa gaaaacaagt aacagaatca agtcatttgg
     6961 aacagcaact tgaagaagct aatgctgtga ggcaagaact agatgatgct tttagacaaa
     7021 tcaaggctta tgaaaaacaa atcaaaacgt tacaacaaga aagagaagat ctaaataagg
     7081 aactagtcca ggctagtgag cgattaaaaa accaatccaa agagctgaaa gacgcacact
     7141 gtcagaggaa actggccatg caggaattca tggagatcaa tgagcggcta acagaattgc
     7201 acacccaaaa acagaaactt gctcgccatg tccgagataa ggaagaagag gtggacctgg
     7261 tgatgcaaaa agttgaaagc ttaaggcaag aactgcgcag aacagaaaga gccaaaaaag
     7321 agctggaagt tcatacagaa gctctagctg ctgaagcatc taaagacagg aagctacgtg
     7381 aacagagtga gcactattct aagcaactgg aaaatgaatt ggagggactg aagcaaaaac
     7441 aaattagtta ctcaccagga gtatgcagca tagaacatca gcaagagata accaaactaa
     7501 agactgattt ggaaaagaaa agtatctttt atgaagaaga attatctaaa agagaaggaa
     7561 tacatgcaaa tgaaataaaa aatcttaaga aagaactgca tgattcagaa ggtcagcaac
     7621 ttgctctcaa caaagaaatt atgattttaa aagacaaatt ggaaaaaacc agaagagaaa
     7681 gtcaaagtga aagggaggaa tttgaaagtg agttcaaaca acaatatgaa cgagaaaaag
     7741 tgttgttaac tgaagaaaat aaaaagctga cgagtgaact tgataagctt actactttgt
     7801 atgagaactt aagtatacac aaccagcagt tagaagaaga ggttaaagat ctagcagaca
     7861 agaaagaatc agttgcacat tgggaagccc aaatcacaga aataattcag tgggtcagcg
     7921 atgaaaagga tgcacgaggg tatcttcagg ccttagcttc taaaatgact gaagaattgg
     7981 aggcattaag aaattccagc ttgggtacac gagcaacaga tatgccctgg aaaatgcgtc
     8041 gttttgcgaa actggatatg tcagctagac tggagttgca gtcggctctg gatgcagaaa
     8101 taagagccaa acaggccatc caagaagagt tgaataaagt taaagcatct aatatcataa
     8161 cagaatgtaa actaaaagat tcagagaaga agaacttgga actactctca gaaatcgaac
     8221 agctgataaa ggacactgaa gagcttagat ctgaaaaggg tatagagcac caagactcac
     8281 agcattcttt cttggcattt ttgaatacgc ctaccgatgc tctggatcaa tttgaacgca
     8341 agactcacca gttttttgta aaatctttta ctactcctac caagtgtcat cagtgtacct
     8401 ccttgatggt gggtttaata agacagggct gttcatgtga agtgtgtgga ttctcatgcc
     8461 atataacttg tgtaaacaaa gctccaacca cttgtccagt tcctcctgaa cagacaaaag
     8521 gtcccctggg tatagatcct cagaaaggaa taggaacagc atatgaaggt catgtcagga
     8581 ttcctaagcc agctggagtg aagaaagggt ggcagagagc actggctata gtgtgtgact
     8641 tcaaactctt tctgtacgat attgctgaag gaaaagcatc tcagcccagt gttgtcatta
     8701 gtcaagtgat tgacatgagg gatgaagaat tttctgtgag ttcagtcttg gcttctgatg
     8761 ttatccatgc aagtcggaaa gatataccct gtatatttag ggtcacagct tcccagctct
     8821 cagcatctaa taacaaatgt tcaatcctga tgctagcaga cactgagaat gagaagaata
     8881 agtgggtggg agtgctgagt gaattgcaca agattttgaa gaaaaacaaa ttcagagacc
     8941 gctcagtcta tgttcccaaa gaggcttatg acagcactct acccctcatt aaaacaaccc
     9001 aggcagccgc aatcatagat catgaaagaa ttgctttggg aaacgaagaa gggttatttg
     9061 ttgtacatgt caccaaagat gaaattatta gagttggtga caataagaag attcatcaga
     9121 ttgaactcat tccaaatgat cagcttgttg ctgtgatctc aggacgaaat cgtcatgtac
     9181 gactttttcc tatgtcagca ttggatgggc gagagaccga tttttacaag ctgtcagaaa
     9241 ctaaagggtg tcaaaccgta acttctggaa aggtgcgcca tggagctctc acatgcctgt
     9301 gtgtggctat gaaaaggcag gtcctctgtt atgaactatt tcagagcaag acccgtcaca
     9361 gaaaatttaa agaaattcaa gtcccatata atgtccagtg gatggcaatc ttcagtgaac
     9421 aactctgtgt gggattccag tcaggatttc taagataccc cttgaatgga gaaggaaatc
     9481 catacagtat gctccattca aatgaccata cactatcatt tattgcacat caaccaatgg
     9541 atgctatctg cgcagttgag atctccagta aagaatatct gctgtgtttt aacagcattg
     9601 ggatatacac tgactgccag ggccgaagat ctagacaaca ggaattgatg tggccagcaa
     9661 atccttcctc ttgttgttac aatgcaccat atctctcggt gtacagtgaa aatgcagttg
     9721 atatctttga tgtgaactcc atggaatgga ttcagactct tcctctcaaa aaggttcgac
     9781 ccttaaacaa tgaaggatca ttaaatcttt tagggttgga gaccattaga ttaatatatt
     9841 tcaaaaataa gatggcagaa ggggacgaac tggtagtacc tgaaacatca gataatagtc
     9901 ggaaacaaat ggttagaaac attaacaata agcggcgtta ttccttcaga gtcccagaag
     9961 aggaaaggat gcagcagagg agggaaatgc tacgagatcc agaaatgaga aataaattaa
    10021 tttctaatcc aactaatttt aatcacatag cacacatggg tcctggagat ggaatacaga
    10081 tcctgaaaga tctgcccatg aaccctcggc ctcaggaaag tcggacagta ttcagtggct
    10141 cagtcagtat tccatctatc accaaatccc gccctgagcc aggccgctcc atgagtgcta
    10201 gcagtggctt gtcagcaagg tcatccgcac agaatggcag cgcattaaag agggaattct
    10261 ctggaggaag ctacagtgcc aagcggcagc ccatgccctc cccgtcagag ggctctttgt
    10321 cctccggagg catggaccaa ggaagtgatg ccccagcgag ggactttgac ggagaggact
    10381 ctgactctcc gaggcattcc acagcttcca acagttccaa cctaagcagc cccccaagcc
    10441 cagtttcacc ccgaaaaacc aagagcctct ccctggagag cactgaccgc gggagctggg
    10501 acccgtcaaa cccagctttc ttgtacaaag tgggcggcaa gcccatcccc aaccccctgc
    10561 tgggcctgga cagcaccgct agcatcccgc tgctgaacgc taaacagatt ctgggacagc
    10621 tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
    10681 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
    10741 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
    10801 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
    10861 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
    10921 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
    10981 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
    11041 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
    11101 ctctggccgc tatgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
    11161 gcctggctcc atatctgtca cggtgggaga agctggataa cttcatcaat agacccgtga
    11221 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
    11281 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
    11341 ggtctgccga aaagtaagcc aactttatta tacatagttg atatccagca cagtggcggc
    11401 cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
    11461 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
    11521 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
    11581 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
    11641 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
    11701 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
    11761 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
    11821 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
    11881 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
    11941 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
    12001 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
    12061 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
    12121 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
    12181 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
    12241 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
    12301 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
    12361 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
    12421 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
    12481 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
    12541 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
    12601 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
    12661 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
    12721 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
    12781 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
    12841 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
    12901 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
    12961 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
    13021 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
    13081 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
    13141 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta
    13201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
    13261 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
    13321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
    13381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    13441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    13501 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
    13561 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    13621 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    13681 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
    13741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    13801 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    13861 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
    13921 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    13981 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    14041 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
    14101 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    14161 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    14221 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    14281 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    14341 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    14401 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    14461 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    14521 gcttacaatt t
//
