LOCUS       sequence-373997-       12245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of SCYL2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12245)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-SCYL2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..12245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5427..5436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8224..8248)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8250..8291
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8298..9068
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(9074..9094)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    9123..9711
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9176..9196)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9594..9605)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9725..10224
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9760..9783)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10157..10176
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10245..10844
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10245..10264)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10741..10761
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10982..11215
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11287..11408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11324..11343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11378..11397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11448..11583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11603..11622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11604..11622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11632..11649)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11632..11648)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11641..11663)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11790..12218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11877..11896)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12087..12108
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatggagtc catgctgaat aagctgaagt
     5461 ctaccgtgac aaaggtgacc gccgacgtga catccgccgt gatgggaaac ccagtgacca
     5521 gggagttcga tgtgggcaga cacatcgcaa gcggaggaaa tggactggca tggaagatct
     5581 ttaacggcac caagaagtcc acaaagcagg aggtggccgt gttcgtgttt gacaagaagc
     5641 tgatcgataa gtaccagaag ttcgagaagg accagatcat cgatagcctg aagaggggag
     5701 tgcagcagct gacaaggctg agacacccta gactgctgac cgtgcagcac ccactggagg
     5761 agtccaggga ctgcctggcc ttctgtacag agccagtgtt tgcctctctg gccaacgtgc
     5821 tgggcaattg ggagaacctg ccttccccaa tctctcccga catcaaggat tacaagctgt
     5881 atgatgtgga gaccaagtat ggcctgctgc aggtgtccga gggcctgtct ttcctgcaca
     5941 gctccgtgaa gatggtgcac ggcaatatca cacctgagaa tatcatcctg aacaagtccg
     6001 gcgcctggaa gatcatgggc ttcgactttt gcgtgtctag caccaaccca tctgagcagg
     6061 agccaaagtt tccctgcaag gagtgggatc ctaatctgcc aagcctgtgc ctgcccaacc
     6121 ctgagtacct ggcccctgag tatatcctgt ccgtgtcttg tgagacagcc agcgacatgt
     6181 actccctggg caccgtgatg tatgccgtgt tcaataaggg caagccaatc tttgaggtga
     6241 acaagcagga catctacaag agcttctcca ggcagctgga tcagctgagc cgcctgggct
     6301 cctctagcct gacaaatatc cccgaggagg tgcgcgagca cgtgaagctg ctgctgaacg
     6361 tgaccccaac agtgcggcct gacgcagatc agatgaccaa gatccctttc tttgacgatg
     6421 tgggcgccgt gacactgcag tatttcgaca ccctgtttca gcgcgataac ctgcagaagt
     6481 cccagttctt taagggcctg cctaaggtgc tgcccaagct gcctaagaga gtgatcgtgc
     6541 agcgcatcct gccatgcctg acaagcgagt tcgtgaatcc agatatggtg ccctttgtgc
     6601 tgcctaacgt gctgctgatc gccgaggagt gtaccaagga ggagtacgtg aagctgatcc
     6661 tgccagagct gggccccgtg ttcaagcagc aggagcctat ccagatcctg ctgatctttc
     6721 tgcagaagat ggacctgctg ctgaccaaga caccccctga tgagatcaag aattctgtgc
     6781 tgccaatggt gtaccgggcc ctggaggcac caagcatcca gatccaggag ctgtgcctga
     6841 atatcatccc cacattcgcc aacctgatcg actatcccag catgaagaat gccctgatcc
     6901 ctagaatcaa gaacgcctgc ctgcagacct cctctctggc cgtgagggtg aactccctgg
     6961 tgtgcctggg caagatcctg gagtacctgg ataagtggtt cgtgctggac gatatcctgc
     7021 cctttctgca gcagatccct agcaaggagc cagccgtgct gatgggcatc ctgggcatct
     7081 ataagtgtac ctttacacac aagaagctgg gcatcaccaa ggagcagctg gcaggcaagg
     7141 tgctgccaca cctgatccct ctgtctatcg agaacaatct gaacctgaat cagttcaata
     7201 gctttatctc cgtgatcaag gagatgctga acagactgga gagcgagcac aagaccaagc
     7261 tggagcagct gcacatcatg caggagcagc agaagagcct ggacatcggc aatcagatga
     7321 acgtgtccga ggagatgaag gtgacaaata tcggcaacca gcagatcgac aaggtgttca
     7381 acaatatcgg cgccgatctg ctgaccggct ctgagagcga gaataaggag gatggcctgc
     7441 agaacaagca caagagggcc tccctgaccc tggaggagaa gcagaagctg gccaaggagc
     7501 aggagcaggc ccagaagctg aagtctcagc agccactgaa gccccaggtg cacaccccag
     7561 tggccacagt gaagcagacc aaggacctga ccgatacact gatggacaat atgagcagcc
     7621 tgacatccct gtctgtgagc acccccaagt ctagcgcctc ctctaccttc acatctgtgc
     7681 ctagcatggg catcggcatg atgttttcca ccccaacaga taatacaaag cgcaatctga
     7741 ccaacggcct gaacgccaat atgggcttcc agacatccgg ctttaacatg cccgtgaaca
     7801 ccaatcagaa cttctacagc tccccttcta ccgtgggcgt gaccaagatg acactgggca
     7861 ccccacccac actgcccaac ttcaacgcac tgagcgtgcc tccagcagga gcaaagcaga
     7921 cacagcagag gcctaccgac atgtccgccc tgaacaatct gttcggccct cagaagccaa
     7981 aggtgtctat gaaccagctg agccagcaga agccaaatca gtggctgaac cagtttgtgc
     8041 cccctcaggg ctctcccaca atgggctcta gcgtgatggg cacccagatg aatgtgatcg
     8101 gccagagcgc cttcggcatg cagggcaacc cattctttaa tccccagaac tttgcccagc
     8161 cacccaccac aatgaccaat tcctctagcg ccagcaacga cctgaaggat ctgttcggct
     8221 caaacccagc tttcttgtac aaagtgggcg gcaagcccat ccccaacccc ctgctgggcc
     8281 tggacagcac cgctagcatc ccgctgctga acgctaaaca gattctggga cagctggacg
     8341 gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg ctggatcgaa
     8401 tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca gggagaggaa
     8461 gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt atgttttggc
     8521 gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt gtcatggcag
     8581 aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat gacctgtatc
     8641 tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca ggcgatgctg
     8701 cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag gaaagcgtgg
     8761 tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg aatactctgg
     8821 ccgctatgct gatccgagag ctgcgggcag ccctggaact gttcgagcag gaaggcctgg
     8881 ctccatatct gtcacggtgg gagaagctgg ataacttcat caatagaccc gtgaagctga
     8941 tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag ggagccctgc
     9001 tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg
     9061 ccgaaaagta agccaacttt attatacata gttgatatcc agcacagtgg cggccgctcg
     9121 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9181 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9241 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9301 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9361 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9421 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9481 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     9541 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9601 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     9661 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     9721 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     9781 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     9841 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     9901 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     9961 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
    10021 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
    10081 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
    10141 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
    10201 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
    10261 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
    10321 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
    10381 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
    10441 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
    10501 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
    10561 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
    10621 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
    10681 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
    10741 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
    10801 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
    10861 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
    10921 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    10981 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    11041 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    11101 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    11161 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
    11221 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
    11281 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    11341 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    11401 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
    11461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    11521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    11581 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
    11641 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    11701 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    11761 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
    11821 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    11881 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    11941 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    12001 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    12061 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    12121 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    12181 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
    12241 aattt
//
