LOCUS       sequence-374157-       11882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of SCYL1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11882)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-SCYL1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..11882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7861..7885)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7887..7928
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7935..8705
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8711..8731)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8760..9348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8813..8833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9231..9242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9362..9861
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9397..9420)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9794..9813
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9882..10481
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9882..9901)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10378..10398
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10619..10852
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10924..11045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10961..10980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11015..11034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11085..11220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11240..11259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11241..11259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11269..11286)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11269..11285)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11278..11300)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11427..11855
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11514..11533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11724..11745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatgtggtt ctttgccaga gaccccgtga
     5461 gggatttccc ttttgagctg atcccagagc cccctgaggg cggcctgcca ggaccttggg
     5521 cactgcacag gggacggaag aaggccaccg gctctcctgt gagcatcttc gtgtacgacg
     5581 tgaagccagg agcagaggag cagacccagg tggcaaaggc cgccttcaag cggttcaaga
     5641 ccctgcggca ccctaacatc ctggcctaca tcgatggcct ggagacagag aagtgcctgc
     5701 acgtggtgac cgaggccgtg acaccactgg gcatctatct gaaggcaagg gtggaggcag
     5761 gaggcctgaa ggagctggag atcagctggg gcctgcacca gatcgtgaag gccctgtcct
     5821 tcctggtgaa cgactgctct ctgatccaca acaacgtgtg catggcagcc gtgtttgtgg
     5881 acagggcagg agagtggaag ctgggaggcc tggattacat gtattctgcc cagggaaatg
     5941 gaggaggacc accaaggaag ggaatcccag agctggagca gtacgaccct ccagagctgg
     6001 ccgatagctc cggcagagtg gtgagggaga agtggagcgc cgatatgtgg aggctgggat
     6061 gcctgatctg ggaggtgttc aacggaccac tgccaagagc agccgccctg aggaatccag
     6121 gcaagatccc caagaccctg gtgccccact attgcgagct ggtgggagca aaccctaagg
     6181 tgcgccctaa tccagcccgg ttcctgcaga actgtagagc cccaggcggc ttcatgagca
     6241 acaggtttgt ggagacaaat ctgtttctgg aggagatcca gatcaaggag cccgccgaga
     6301 agcagaagtt ctttcaggag ctgagcaagt ccctggacgc cttcccagag gatttttgta
     6361 gacacaaggt gctgccccag ctgctgaccg ccttcgagtt tggcaatgcc ggcgccgtgg
     6421 tgctgacacc actgttcaag gtcggcaagt ttctgtccgc cgaggagtac cagcagaaga
     6481 tcatccccgt ggtggtgaag atgttctcta gcaccgacag agccatgaga atcaggctgc
     6541 tgcagcagat ggagcagttt atccagtatc tggacgagcc caccgtgaac acacagatct
     6601 tccctcacgt ggtgcacggc tttctggata ccaatcctgc catcagggag cagacagtga
     6661 agagcatgct gctgctggcc ccaaagctga acgaggccaa cctgaatgtg gagctgatga
     6721 agcacttcgc aaggctgcag gcaaaggacg agcagggccc tatccggtgc aataccaccg
     6781 tgtgcctggg caagatcggc tcctacctgt ctgccagcac caggcaccgg gtgctgacat
     6841 ccgccttctc tagggcaacc agggacccct ttgccccttc cagggtggca ggcgtgctgg
     6901 gatttgcagc cacacacaac ctgtattcta tgaatgattg cgcccagaag atcctgcccg
     6961 tgctgtgcgg actgaccgtg gacccagaga agagcgtgcg cgatcaggcc ttcaaggcca
     7021 tccggagctt tctgtccaag ctggagtccg tgtctgagga ccccacacag ctggaggagg
     7081 tggagaagga tgtgcacgcc gcctcctctc ctggcatggg aggagcagca gcatcctggg
     7141 caggatgggc cgtgaccggc gtgagcagcc tgacatctaa gctgatcaga agccacccta
     7201 ccacagcccc aaccgagaca aacatccctc agaggccaac ccctgaggga gtgccagcac
     7261 cagcccctac cccagtgccc gccacaccaa ccacatccgg ccactgggag acacaggagg
     7321 aggacaagga taccgccgag gactctagca cagccgatag atgggacgat gaggactggg
     7381 gaagcctgga gcaggaggca gagtccgtgc tggcccagca ggacgattgg tctaccggag
     7441 gacaggtgtc tagggcaagc caggtgagca attccgacca caagtcctct aagtcccccg
     7501 agtctgattg gagcagctgg gaggcagagg gaagctggga gcagggctgg caggagcctt
     7561 ctagccagga gccacctcca gatggaacca ggctggcctc cgagtataac tggggaggac
     7621 cagagtcctc tgacaagggc gatcccttcg ccaccctgag cgccagacct tccacacagc
     7681 ctaggccaga ctcttggggc gaggataatt gggagggact ggagaccgac agcaggcagg
     7741 tgaaggcaga gctggcaaga aagaagaggg aggagcggag aagggagatg gaggcaaaga
     7801 gggcagagag gaaggtggca aagggaccaa tgaagctggg cgccagaaag ctggattcaa
     7861 acccagcttt cttgtacaaa gtgggcggca agcccatccc caaccccctg ctgggcctgg
     7921 acagcaccgc tagcatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg
     7981 ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg
     8041 gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca
     8101 gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc
     8161 tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag
     8221 cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc
     8281 aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac
     8341 agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca
     8401 atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg
     8461 ctatgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc
     8521 catatctgtc acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca
     8581 ttggggacaa agagattttc gggattagcc gggggattga taaacaggga gccctgctgc
     8641 tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg
     8701 aaaagtaagc caactttatt atacatagtt gatatccagc acagtggcgg ccgctcgaca
     8761 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8821 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8881 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8941 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     9001 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     9061 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     9121 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     9181 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     9241 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     9301 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac
     9361 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     9421 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     9481 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     9541 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     9601 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     9661 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     9721 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     9781 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     9841 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     9901 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     9961 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
    10021 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    10081 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    10141 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    10201 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    10261 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    10321 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    10381 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    10441 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
    10501 agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga
    10561 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
    10621 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
    10681 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10741 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10801 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
    10861 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
    10921 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10981 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    11041 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
    11101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    11161 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    11221 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
    11281 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    11341 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    11401 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
    11461 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    11521 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    11581 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    11641 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    11701 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    11761 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    11821 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    11881 tt
//
